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10 - Generation of New Model Cell Lines using ssODN Knockin Donors and FACS-based Genome Editing

from Part II - Genome Editing in Model Organisms

Published online by Cambridge University Press:  30 July 2018

Krishnarao Appasani
Affiliation:
GeneExpression Systems, Inc.
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Summary

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Type
Chapter
Information
Genome Editing and Engineering
From TALENs, ZFNs and CRISPRs to Molecular Surgery
, pp. 150 - 162
Publisher: Cambridge University Press
Print publication year: 2018

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References

Chen, F, Pruett-Miller, SM, Huang, Y, et al. 2011. High frequency genome editing using ssDNA oligos and zinc finger nucleases. Nat Methods 8: 753755.CrossRefGoogle Scholar
Duda, K, Lonowski, LA, Kofoed-Nielsen, M, et al. 2014. High-efficiency genome editing via 2A-coupled co-expression of zinc finger nucleases or CRISPR/Cas nickase pairs. Nucleic Acids Res 42(10): e84.CrossRefGoogle ScholarPubMed
Frank, S, Skryabin, BV, Greber, B. 2013. A modified TALEN-based system for robust generation of knock-out human pluripotent stem cell lines and disease models. BMC Genomics 14: 773.CrossRefGoogle ScholarPubMed
Fu, XD, Ares, M Jr. 2014. Context-dependent control of alternative splicing by RNA-binding proteins. Nat Rev Genet 15(10): 689701.CrossRefGoogle ScholarPubMed
Hodgkins, A, Farne, A, Perera, S, et al. 2015. WGE: a CRISPR database for genome engineering. Bioinformatics 31(18): 30783080.CrossRefGoogle ScholarPubMed
Lonowski, LA, Narimatsu, Y, Riaz, A, et al. 2017. Genome editing using FACS enrichment of nuclease expressing cells and Indel Detection by Amplicon Analysis, Nat Protocols 12(3): 581603.CrossRefGoogle ScholarPubMed
Ran, FA, Hsu, PD, Wright, J, et al. 2013. Genome engineering using the CRISPR-Cas9 system. Nat Protocols 8(11): 22812308.CrossRefGoogle ScholarPubMed
Renaud, JB, Boix, C, Charpentier, M, et al. 2016. Improved genome editing efficiency and flexibility using modified oligonucleotides with TALEN and CRISPR-Cas9 nucleases. Cell Reports 14(9): 22632272.CrossRefGoogle ScholarPubMed
Richardson, CD, Ray, GJ, DeWitt, M, Curie, GL, Corn, JE. 2016. Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Nat Biotech 34: 339344.CrossRefGoogle ScholarPubMed
Szymczak, AL, Vignali, DA. 2005. Development of 2 A peptide-based strategies in the design of multicistronic vectors. Expert Opin Biol Ther 5(5): 627638.CrossRefGoogle Scholar
Yang, L, Guell, M, Byrne, S, et al. 2013. Optimization of scarless human stem cell genome editing. Nucleic Acids Res 41: 90499061.CrossRefGoogle ScholarPubMed
Yang, Z, Steentoft, C, Hauge, C, et al. 2015. Fast and sensitive detection of indels induced by precise gene targeting. Nucleic Acids Res 43(9): e59.CrossRefGoogle ScholarPubMed

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