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Chapter 7 - Sperm Vitality: Hypo-Osmotic Swelling Test

Published online by Cambridge University Press:  05 April 2021

Ashok Agarwal
Affiliation:
The Cleveland Clinic Foundation, Cleveland, OH
Ralf Henkel
Affiliation:
University of the Western Cape, South Africa
Ahmad Majzoub
Affiliation:
Hamad Medical Corporation, Doha
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Summary

The hypo-osmotic swelling (HOS) test, introduced in 1987 to assess the functional integrity of the human sperm membrane, appears to have withstood the test of time. Many articles have been published on the subject since its inclusion in the third edition of the World Health Organization (WHO) manual in 1992 [1, 2, 3, 4, 5].

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Publisher: Cambridge University Press
Print publication year: 2021

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References

World Health Organization. (1992) WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction. Cambridge: Cambridge University Press.Google Scholar
Check, JH, Epstein, R, Nowroozi, K, Shanis, BS, Wu, CH, Bollendorf, A. The hypoosmotic swelling test as a useful adjunct to the semen analysis to predict fertility potential. Fertil Steril 1989; 52(1): 159–61CrossRefGoogle ScholarPubMed
Casper, RF, Meriano, JS, Jarvi, KA, Cowan, L, Lucato, ML. The hypo-osmotic swelling test for selection of viable sperm for intracytoplasmic sperm injection in men with complete asthenozoospermia. Fertil Steril 1996; 65(5): 972–6.CrossRefGoogle ScholarPubMed
Ahmadi, A, Ng, SC. The single sperm curling test, a modified hypo-osmotic swelling test, as a potential technique for the selection of viable sperm for intracytoplasmic sperm injection. Fertil Steril 1997; 68(2): 346–50.CrossRefGoogle ScholarPubMed
Pang, MG, You, YA, Park, YJ, Oh, SA, Kim, DS, Kim, YJNumerical chromosome abnormalities are associated with sperm tail swelling patternsFertil Steril 2010; 94: 1012–20.CrossRefGoogle ScholarPubMed
Jeyendran, RS, Van der Ven, HH, Perez-Pelaez, M,Crabo, BG, Zaneveld, LJD. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 1984; 70: 219–28.Google ScholarPubMed
Cheng, X, Pinsky, PM. The balance of fluid and osmotic pressures across active biological membranes with application to the corneal endothelium. PLOS ONE 2015; 10: e0145422.CrossRefGoogle ScholarPubMed
Schrader, SM, Platek, SF, Zaneveld, LJD, et al. Sperm viability: a comparison of analytical methods. Andrologia 1986; 18: 530–8.Google ScholarPubMed
Bollendorf, A, Check, JH, Kramer, D. The majority of males with subnormal hypoosmotic test scores have normal vitality. Clin Exp Obstet Gynecol 2012; 39(1): 25–6.Google ScholarPubMed
Ramu, S, Jeyendran RS. The hypo-osmotic swelling test for evaluation of sperm membrane integrity. Methods Mol Biol 2013; 927: 21–5.CrossRefGoogle ScholarPubMed
Van der Ven, HH, Jeyendran, RS, Al-Hasani, S, Perez-Pelaez, M, Diedrich, K, Zaneveld, LJD. Correlation between human sperm swelling in hypoosmotic medium (hypoosmotic swelling test) and in vitro fertilization. J Androl 1986; 7: 190–6.CrossRefGoogle ScholarPubMed
Schrader, SM, Turner, TW, Breitenstein, MJ, Simon, SD. Longitudinal study of semen quality of unexposed workers. I. Study overview. Reprod Toxicol 1988; 2(3–4): 183–90.Google ScholarPubMed
de Castro, M, Jeyendran, RS, Zaneveld, LJD. Hypoosmotic swelling test: analysis of prevasectomy ejaculates. Arch Androl 1990; 24: 1116.CrossRefGoogle Scholar
Van Kooij, RT, Balerna, M, Roatti, A, Campana, Z. Oocyte penetration and acrosome reactions of human sperms. II: Correlation with other seminal parameters. Andrologia 1986; 18: 503–8.Google ScholarPubMed
Check, JH, Nowroozi, K, Wu, CH, Bollendorf, A. Correlation of semen analysis and hypoosmotic swelling test with subsequent pregnancies. Arch Androl 1988; 20: 257–60.Google ScholarPubMed
Check, ML, Check, JH. Poor hypo-osmotic swelling test results from cryopreserved sperm despite preservation of sperm motility. Arch Androl 1991; 26(1): 3741.CrossRefGoogle ScholarPubMed
Tartagni, M, Cicinelli, E, Schonauer, MM, Causio, F, Petruzzelli, F, Loverro, G. Males with subnormal hypo-osmotic swelling test scores have lower pregnancy rates than those with normal scores when ovulation induction and timed intercourse is used as a treatment for mild problems with sperm count, motility, or morphology. J Androl 2004; 25(5): 781–3.CrossRefGoogle ScholarPubMed
Check, JH, Aly, J. Sperm with an abnormal hypo-osmotic swelling testnormal fertilization, normal embryo development, but implantation failure. Clin Exp Obstet Gynecol 2016; 43(3): 319–27.Google ScholarPubMed
De Jonge, C. Semen analysis: looking for an upgrade in class. Fertil Steril 2012; 97: 260–6.CrossRefGoogle ScholarPubMed
Jeyendran, RS, Van der Ven, HH, Zaneveld, LJD. The hypoosmotic swelling test: an update. Arch Androl 1992; 29: 105–16.CrossRefGoogle ScholarPubMed
Wang, C, Chan, SYW, Ng, M, So, WWK, Tsoi, W, Lo, T, Leung, A. Diagnostic value of sperm function test and routine semen analysis in fertile and infertile men. J Androl 1988; 9: 384–9.CrossRefGoogle ScholarPubMed
Coetzee, K, Krunger, FT, Menkveld, R, Lombard, CJ, Swanson, RJ. Hypoosmotic swelling test in the prediction of male fertility. Arch Androl 1989; 23: 131–8.Google ScholarPubMed
Jeyendran, RS, Van der Ven, HH, Rachagan, SP, Perez-Plaeaz, M, Zaneveld, LJD. Semen quality and in-vitro fertilization. Aust NZ J Obsetet Gynecol 1989; 29: 168–72.Google ScholarPubMed
Check, JH, Stumpo, L, Lurie, D, Benfer, K, Callan, CA. Comparative prospective study using matched samples to determine the influence of subnormal hypo-osmotic test scores of spermatozoa on subsequent fertilization and pregnancy rates following in-vitro fertilization. Hum Reprod 1995; 10(5): 1197–200.CrossRefGoogle ScholarPubMed
Mladenović, I, Mićić, S, Genbaćev, O, Papić, N. Hypoosmotic swelling test for quality control of sperm prepared for assisted reproduction. Arch Androl 1995; 4(3): 163–9.Google Scholar
Tartagni, M, Schonauer, MM, Cicinelli, E, Selman, H, De Ziegler, D, Petruzzelli, F, D'Addario, V. Usefulness of the hypo-osmotic swelling test in predicting pregnancy rate and outcome in couples undergoing intrauterine insemination. J Androl 2002; 23(4): 498502.Google ScholarPubMed
Datta, S, Giri, A, Datta, AK. Role of hypo-osmotic sperm swelling test in assisted reproduction. J Indian Med Assoc 1996; 94(12): 440–2.Google ScholarPubMed
Kiefer, D, Check, JH, Katsoff, D. The value of motile density, strict morphology, and the hypoosmotic swelling test in in vitro fertilization-embryo transfer. Arch Androl 1996; 37(1): 5760.CrossRefGoogle ScholarPubMed
Jedrzejczak, P, Taszarek-Hauke, G, Hauke, J, Pawelczyk, L, Duleba, AJ. Prediction of spontaneous conception based on semen parameters. Int J Androl 2008; 31(5): 499507.CrossRefGoogle ScholarPubMed
Patankar, SS, Deshkar, AM, Sawane, MV, Mishra, NV, Kale, AH, Gosavi, GB. The role of hypo-osmotic swelling test in recurrent abortions. Indian J Physiol Pharmacol 2001; 45(3): 373–7.Google ScholarPubMed
Saxena, P, Misro, MM, Chaki, SP, Chopra, K, Roy, S, Nandan, D. Is abnormal sperm function an indicator among couples with recurrent pregnancy loss? Fertil Steril 2008; 90(5): 1854–8.CrossRefGoogle ScholarPubMed
Bhattacharya, SM. Hypo-osmotic swelling test and unexplained repeat early pregnancy loss. J Obstet Gynaecol Res 2010; 36(1): 119–22.CrossRefGoogle ScholarPubMed
Kohn, FM, Schuppe, HC, Schill, WB, Jeyendran, RS. Hydrogen hexachloroplatinate induces the acrosome reaction in human spermatozoa. Int J Androl 1995; 18(6): 321–5.CrossRefGoogle ScholarPubMed
Reiter, WJ, Tomek, S, Zielinski, CC, Marberger, M. Effect of carboplatin on the functional integrity of the human sperm membrane in vitro. J Androl 2002; 23(3): 338–40.Google ScholarPubMed
Ratcliffe, JM, Schrader, SM, Steenland, K, Clapp, DE, Turner, T, Hornung, RW. Semen quality in papaya workers with long term exposure to ethylene dibromide. Br J Ind Med 1987; 44(5): 317–26.Google ScholarPubMed
Ratnasooriya, WD, Jayawardena, KGI, Wadsworth, RM. Effects of enalapril on human sperm motility. Contracept 1990; 41(2): 213–19.CrossRefGoogle ScholarPubMed
Ratnasooriya, WD, Premakumara, GAS. Effects of the prostanoid receptor antagonist, di-4-phloretin phosphate, upon human sperm motility. Contracept 1992; 45(3): 239–48.CrossRefGoogle ScholarPubMed
Hossain, AM, Selukar, R, Barik, S. Differential effect of common laboratory treatments on hypoosmotic swelling responses of human spermatozoa. J Assist Reprod Genet 1999; 16(1): 30–4.CrossRefGoogle ScholarPubMed
Jeyendran, RS, Van der Ven, HH, Perez-Pelaez, M, Zaneveld, LJD. Nonbeneficial effects of glycerol on the oocyte penetrating capacity of cryopreserved and incubated human spermatozoa. Cryobiology 1985; 22(5): 434–7.CrossRefGoogle ScholarPubMed
Bachtell, NE, Conaghan, J, Turek, PJ. The relative viability of human spermatozoa from the vas deferens, epididymis and testis before and after cryopreservation. Hum Reprod 1999; 14(12): 3048–51.CrossRefGoogle ScholarPubMed
Hossain, AM, Rizk, B, Barik, S, Huff, C, Thorneycroft, IH. Time course of hypo-osmotic swellings of human spermatozoa: evidence of ordered transition between swelling subtypes. Hum Reprod 1998; 13: 1578–83.CrossRefGoogle ScholarPubMed
Chan, SW, Wang, C, Ng, M, et al. Evaluation of computerized analysis of sperm movement characteristics and differential sperm tail swelling patterns in predicting human sperm in vitro fertilizing capacity. J Androl 1989; 10: 133–8.CrossRefGoogle ScholarPubMed
Bassiri, F, Tavalaee, M, Shiravi, AH, Mansouri, S, Nasr-Esfahani, MH. Is there an association between HOST grades and sperm quality? Hum Reprod 2012; 27(8): 2277–84.CrossRefGoogle ScholarPubMed
Stranger, JD, Vo, L, Yovich, JL, Almahbobi, G. Hypo-osmotic swelling test identifies individual spermatozoa with minimal DNA fragmentation. Reprod Biomed Online 2010; 21: 474–84.Google Scholar
Rouen, A, Carlier, LHeide, SEgloff, MMarzin, PAder, FSchwartz, MRogers, EJoyé, NBalet, RLédée, NPrat-Ellenberg, LCassuto, NGSiffroi, JP. Potential selection of genetically balanced spermatozoa based on the hypo-osmotic swelling test in chromosomal rearrangement carriers. Reprod Biomed Online 2017; 35(4): 372–8.CrossRefGoogle ScholarPubMed
Liu, J, Tsai, YL, Katz, E, Compton, G, Garcia, JE, Baramki, TA. High fertilization rate obtained after intracytoplasmic sperm injection with 100% nonmotile spermatozoa selected by using a simple modified hypo-osmotic swelling test. Fertil Steril 1997; 68(2): 373–5.CrossRefGoogle ScholarPubMed
Vandervorst, M, Tournaye, H, Camus, M, Nagy, ZP, Van Steirteghem, A, Devroey, P. Patients with absolutely immotile spermatozoa and intracytoplasmic sperm injection. Hum Reprod 1997; 12: 2429–33.CrossRefGoogle ScholarPubMed
Ved, S, Montag, M, Schmutzler, A, Prietl, G, Haidl, G, van der Ven, H. Pregnancy following intracytoplasmic sperm injection of immotile spermatozoa selected by the hypo-osmotic swelling-test: a case report. Andrologia 1997; 29(5): 241–2.Google ScholarPubMed
Sallam, H, Farrag, A, Agameya, A, Ezzeldin, F, Eid, A, Sallam, A. The use of a modified hypo-osmotic swelling test for the selection of viable ejaculated and testicular immotile spermatozoa in ICSI. Hum Reprod 2001; 16(2): 272–6.CrossRefGoogle ScholarPubMed
Westlander, G, Barry, M, Petrucco, O, Norman, R. Different fertilization rates between immotile testicular spermatozoa and immotile ejaculated spermatozoa for ICSI in men with Kartagener's syndrome: case reports. Hum Reprod 2003; 18(6): 1286–8.CrossRefGoogle ScholarPubMed
Peeraer, K, Nijs, M, Raick, D, Ombelet, W. Pregnancy after ICSI with ejaculated immotile spermatozoa from a patient with immotile cilia syndrome: a case report and review of the literature. Reprod Biomed Online 2004; 9(6): 659–63.CrossRefGoogle ScholarPubMed
Sallam, HN, Farrag, A, Agameya, AF, El-Garem, Y, Ezzeldin, F. The use of the modified hypo-osmotic swelling test for the selection of immotile testicular spermatozoa in patients treated with ICSI: a randomized controlled study. Hum Reprod 2005; 20(12): 3435–40.CrossRefGoogle ScholarPubMed
World Health Organization. (2010) WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction. Cambridge: Cambridge University Press.Google Scholar
Jeyendran, RS, Zaneveld, LJD. Controversies in the developments and validation of new sperm assays. Fertil Steril 1993; 59: 726–8.CrossRefGoogle Scholar

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