Mature fruits from 21 mango, Mangifera indica L., cultivare grown in the Miami National Clonal Germplasm Repository were examined for resistance under laboratory conditions to identify those resistant (antibiotic or antixenotic) to the eggs and larvae of the Caribbean fruit fly, Anastrepha suspensa (Leow). The bioassay consisted of artificially infesting a slice of fruit with eggs obtained from a laboratory colony and counting the adults that emerged. Emergence ranged from 26.6% (on Tobago Small Red) to 119.0% (on Sabre) of controls (on artifical diet). Tobago Small Red, Becky, Saigon Seedling, Zilate, Sandersha, 13–1, Keitt, Turpentine, Rumani, Tommy Atkins, Irwin and Peach had the highest level of resistance observed with less than 80% emergence. The most resistant cultivars are promoted to breeders and growers for production in areas under quarantine for Caribbean fruit fly.