Detached leaves and whole plants of quackgrass [Agropyron repens (L.) Beauv. # AGRRE] were used to study uptake and translocation of butyl esters of 14C-fluazifop {(±)-2-[4-[[5-(trifluoromethyl)-2-pyridinyl] oxy] phenoxy] propanoic acid}, with or without additional adjuvants. In the absence of adjuvants 3.2% of applied radioactivity entered detached quackgrass leaves by 6 h, and at the end of 24 h, 6.0% had penetrated. The presence of additives increased uptake by leaves significantly. In the presence of the nonionic surfactant Agral (nonyl phenol ethoxylate) at 0.2% (v/v) or the oil-additive Actipron (self-emulsifying adjuvant oil) at 2.0% (v/v), 17.2 and 12.9% of applied radioactivity, respectively, entered the leaves by 24 h. Evidence of dependence of phloem translocation of the radioactivity on source-sink relationships of the plant was obtained in the studies with whole plants. Translocation measured up to 7 days after treatment showed that radioactivity was concentrated in areas such as young developed leaves, young stems, and rhizome apices. Rhizomes appear to be major sinks for the accumulation of radioactivity and at 7 days 0.5% of applied radioactivity was found there. In wholeplant experiments the two adjuvants either individually or in mixture increased the uptake of 14C-fluazifop significantly. However, a corresponding increase in basipetal translocation was found only in one experiment. Much of the increased activity that had entered the leaves in the presence of adjuvants was found to have moved to areas distal to the treated zone or remained within the treated zones. In all experiments, applied 14C was not fully recovered. Evidence of significant volatility losses from treated surfaces was obtained and it is thought that this may be the main reason for the inability to recover all of the applied activity.

Established stands of alfalfa (Medicago sativa L.) at two field locations were treated at one date in the fall with pronamide [3,5-dichloro (N-1,1-dimethyl-2-propynil) benzamide] at 0.8 and 1.6 kg ai/ha and at two dates in the spring with sethoxydim {2-[1-(ethoxyimino)butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one} at 0.3 and 0.6 kg ai/ha for the control of quackgrass [Agropyron repens (L.) Beauv. # AGRRE]. Herbicide efficacy was compared by measuring botanical and chemical composition and yield of forage. Both chemicals suppressed quackgrass in first-cutting herbage. While sethoxydim at the higher rate was equivalent to pronamide for increasing the proportion of alfalfa in forage, pronamide was superior for increasing seasonal total yield. Late postemergence applications of sethoxydim were more effective for quackgrass regrowth control than earlier spring applications. Controlling quackgrass in late spring resulted in lower crude protein (CP) content in first-cutting forage than did controlling it in fall. Alfalfa competing with untreated quackgrass had a lower CP content than where quackgrass was controlled with herbicides. All herbicide treatments reduced the neutral-detergent fiber (NDF) of the herbage. As measured by the acid-detergent fiber (ADF) concentration of alfalfa, sethoxydim slightly retarded spring growth of the legume.

Greenhouse and field experiments were conducted to examine foliar absorption and activity of nicosulfuron and primisulfuron by quackgrass with various adjuvants. Foliar absorption of 14C-nicosulfuron and 14C-primisulfuron plus petroleum oil adjuvant (POA) was completed by 4 h after application. Absorption of nicosulfuron plus POA and primisulfuron plus POA increased from 11 and 2% of applied, respectively, to 51 and 12% with the addition of urea-ammonium nitrate liquid fertilizer (UAN). At least 83% of the absorbed 14C from either herbicide penetrated the leaf epicuticular waxes. Absorption of 14C-labeled herbicides was greatest with the following adjuvants: POA + UAN > nonionic surfactant (NIS) + UAN = methylated seed oil. The addition of UAN to either NIS or POA significantly increased 14C-herbicide uptake. In greenhouse studies, nicosulfuron and primisulfuron applied with POA plus UAN provided greater quackgrass control than with POA alone. Despite the differences in foliar uptake in the greenhouse, few differences were observed between these adjuvants in 1989 or 1990 field efficacy trials. Quackgrass control was reduced by the addition of atrazine to nicosulfuron plus POA in 1989 and to primisulfuron plus POA in 1990. Combinations with atrazine did not reduce 14C-nicosulfuron or 14C-primisulfuron uptake by quackgrass.

Translocation of 14C-haloxyfop {2-[4-[[3-chloro-5-(trifluoromethyl)-2-pyridinyl] oxy] phenoxy] propanoic acid} in quackgrass [Agropyron repens (L.) Beauv. # AGRRE] from the treated area (the middle 2.5 cm of the second of three leaves) to the lower leaves was reduced in plants in which 0.56 kg/ha of acifluorfen {5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acid} or bentazon [3-1-methylethyl)-(1H)-2,1,3-benzothiadiazin-4(3H)-one-2,2-dioxide] was added in tank mixture to a 0.07 kg ai/ha application of haloxyfop. Reduced translocation did not occur when haloxyfop rates were increased to 0.28 and 1.1 kg/ha. Bentazon reduced absorption of 14C-DPX-Y6202 {2-[4-[(6-chloro-2-quinoxalinyl)oxy] phenoxy] propionic acid} when applied in tank mixtures with 0.28 and 1.1 kg ai/ha DPX-Y6202. There was less 14C-DPX-Y6202 in the leaf tips of plants treated with 0.07 kg/ha DPX-Y6202 plus 0.56 kg/ha acifluorfen in tank mixture. Relatively small amounts of both 14C-haloxyfop and DPX-Y6202 accumulated in the rhizome and adjacent shoots and acifluorfen did not influence translocation to these plant parts. Considerably more 14C-haloxyfop than 14C-DPX-Y6202 was absorbed by quackgrass. Treatment of quackgrass with acifluorfen or bentazon 24 h prior to application of 14C-haloxyfop or 14C-DPX-Y6202 did not influence absorption or translocation.

Spray solutions containing a cationic or a nonionic surfactant were applied to six plant species at a broad range of concentrations. The species investigated were three weeds (black nightshade, chamomile, and quackgrass) and three crops (winter wheat, pea, and tomato). The microroughness of the leaf surface as revealed by scanning electron microscopy appeared to be a relevant retention-determining factor. Plant species with crystalline epicuticular waxes (winter wheat, pea, and quackgrass) retained much less spray solution than the other species, which are characterized by a smooth cuticular surface. The two surfactants enhanced retention on species with a reflective surface, whereas retention on black nightshade, chamomile, and tomato was hardly influenced by addition of surfactants. The two surfactants had a similar influence on the retention. Surfactant at 1% (wt/v) enhanced retention on pea, winter wheat, and quackgrass by factors of twenty, six, and four, respectively, compared with retention without surfactant. A linear relation between retention and logarithm of surfactant concentration was observed. Retention of spray drops was related not to equilibrium surface tension of the spray solution but rather to dynamic surface tension.

In experiments conducted under controlled conditions, glyphosate-induced inhibition of rhizome bud growth in quackgrass was reduced by increasing the nitrogen (N) concentration in the nutrient solution from 10.5 to 210 mg L−1 either 2 or 4 d before foliar application of the herbicide, and for 7 d after the herbicide treatment. The additional N reduced the glyphosate-induced inhibition of rhizome growth on the intact plant at the lowest glyphosate dosage (2.8 μg per plant) but had no significant effect at higher dosages. Both the 2- and 4-d high N treatments significantly increased the length, dry weight, and water content of the lateral buds by the time the herbicide was applied. They also increased bud growth on the glyphosate-treated plants during the 7 d following the herbicide application and promoted release of the buds from inhibition during a subsequent bioassay of their regenerative growth on excised, single-node rhizome segments. These effects of N supply interacted significantly with glyphosate dosage, bud position on the rhizome, and duration of incubation. It is postulated that bud growth response to increased N supply may reduce the inhibiting effect of the herbicide by diluting the glyphosate concentration in the bud, or that the additional N may counteract the toxic action of glyphosate on amino acid metabolism or protein synthesis.

Field trials indicated that addition of ammonium sulfate to imazethapyr plus nonionic surfactant increased quackgrass control, especially at low imazethapyr rates. In greenhouse experiments, approximately twice as much imazethapyr was absorbed by quackgrass leaves when the herbicide was applied in combination with nonionic surfactant plus ammonium sulfate than when the herbicide was applied with nonionic surfactant alone. Black Mexican Sweet maize (BMS) suspension-cultured cells were used to evaluate the effects of ammonium sulfate and nonionic surfactant on cellular absorption of imazethapyr in the absence of a cuticular barrier. Imazethapyr absorption by BMS cells was diffusion-mediated, energy-dependent, and exhibited a pH optimum of approximately 3. Over the concentration range of 0.1 to 10.0 μM, the equilibrium concentration of imazethapyr in BMS cells was a linear function of the external concentration. Addition of ammonium sulfate to the external medium of BMS cells enhanced both the rate of imazethapyr uptake and medium acidification. There was a linear correlation between the ability of ammonium sulfate (0.5 to 10 mM) to promote medium acidification and imazethapyr uptake by BMS cells. The ammonium sulfate-induced stimulation of imazethapyr absorption in BMS cells was sensitive to plasma membrane adenosine triphosphatase inhibitors (sodium vanadate, diethylstilbestrol), the uncoupler carbonyl cyanide m-chlorophenylhydrazone, and energy metabolism inhibitors (sodium azide, nitrogen gas), demonstrating that this effect was dependent on ATP production and the functioning of the plasma membrane ATPase. It is hypothesized that cytoplasmic acidification in BMS cells due to ammonium assimilation stimulates the plasma membrane ATPase to pump protons across the plasma membrane which in turn acidifies the cell wall promoting cellular accumulation of imazethapyr by ion-trapping. Cell wall acidification due to ammonium assimilation may contribute to the ability of ammonium sulfate to enhance the efficacy of imazethapyr and other foliar-applied herbicides.

Between 1982 and 1986, spring treatments of haloxyfop, fluazifop, and sethoxydim all applied at 400 g ai/ha alone and combined with dalapon at 3400 g ai/ha were evaluated for quackgrass control and subsequent effect on established birdsfoot trefoil seed production. Haloxyfop alone and combined with dalapon were superior to fluazifop or sethoxydim alone or mixed with dalapon for controlling quackgrass and other grass weeds. Birdsfoot trefoil seed yield averages over 3 yr were 310, 160, 150, and 180 kg/ha after treatment with haloxyfop, fluazifop, sethoxydim, and dalapon, respectively, compared to a control yield of 80 kg/ha. Yields were lower, 220 kg/ha, with combinations involving haloxyfop plus dalapon compared to haloxyfop alone.

In laboratory and field studies, quackgrass (Agropyron repens (L.) Beauv. # AGRRE) was subjected to postemergence applications of the butyl ester of fluazifop-P {(R)-2-[4-[[5-(trifluoromethyl)-2-pyridinyl] oxy] phenoxy] propanoic acid}, methyl ester of haloxyfop {2-[4-[[3-chloro-5-(trifluoromethyl)-2-pyridinyl] oxy] phenoxy] propanoic acid}, or ethyl ester of quizalofop {2-[4-[[6-(chloro-2-quinoxalinyl)oxy] phenoxy] propanoic acid}. Haloxyfop, fluazifop-P, and quizalofop each required a minimum of 1 week to reduce quackgrass transpiration or water uptake in the laboratory. At rates lower than 0.56 kg ae/ha, fluazifop-P reduced quackgrass transpiration and water usage less quickly and to a lesser extent than equivalent rates of haloxyfop and quizalofop. The time required for significant transpiration and water uptake reduction by herbicide-treated quackgrass was several days longer in the field than in the laboratory, but the reaction patterns among the herbicides were similar. Little if any benefit was obtained from sequential applications of the herbicides compared with single applications of the same total dosage. All three herbicides significantly reduced quackgrass dry weight.

Soybean-based [Glycine max (L.) Merr.] and petroleum-based crop oil concentrates were equally effective as spray adjuvants for control of quackgrass [Agropyron repens (L.) Beauv. #3 AGRRE] with the butyl ester of fluazifop {[±)-2-[4-[[5-(trifluoromethyl)-2-pyridinyl] oxy] phenoxy] propanoic acid} and sethoxydim {2-[1-(ethoxyimino)butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one}. With favorable growing conditions at application in 1983 and 1985, an adjuvant concentration of 1% (v/v) with these herbicides was adequate for quackgrass control. Unfavorable growing conditions at application in 1984 resulted in unacceptable quackgrass control, and spray volume did not affect control. However, increasing the adjuvant concentration to 4% (v/v) with either herbicide applied in a spray volume of 70 L/ha nearly doubled quackgrass control. In 1983 and 1985, equal or greater quackgrass control with each herbicide was achieved with spray volumes as low as 35 L/ha compared to 280 L/ha.

The influence of adjuvants, inorganic salts, and herbicide formulations on the antagonistic effect of Na-bentazon on sethoxydim absorption was studied in quackgrass. Adjuvant BCH 815 00, at 2.4 L ha−1 alone or at 1.2 L ha−1 combined with 1.1 kg ha−1 of ammonium sulfate, added to the sethoxydim and Na-bentazon spray mixture overcame the antagonism on 14C-sethoxydim absorption in quackgrass. Crop oil concentrate at rates up to 9.6 L ha−1 did not overcome the antagonism. Adding ammonium phosphate or ammonium nitrate to the spray solution was as effective as adding ammonium sulfate in overcoming the antagonism caused by reduced absorption of sethoxydim even from the combination of Na-bentazon plus Na-acifluorfen. Ammonium salts and NH4-bentazon overcame the antagonism of Na-bentazon by forming readily absorbed NH4-sethoxydim.

Aqueous extracts of quackgrass [Agropyron repens (L.) Beauv. # AGRRE] shoots and rhizomes inhibited seed germination and root growth of alfalfa (Medicago sativa L. ‘Vernal’), soybean (Glycine max (L.) Merr. ‘Corsoy 79’], navybean (Phaseolus vulgaris L. ‘Seafarer’), and curly cress (Lepidium sativum L.) at concentrations of less than 2.5 mg dried extract/ml. Extracts of quackgrass shoots were generally more inhibitory than extracts of rhizomes. Root and shoot dry weights of snapbeans (Phaseolus vulgaris L. ‘Bush Blue Lake’) grown under sterile conditions were reduced by aqueous extracts of shoots. Root systems were stunted and necrotic and lacked root hairs. The growth of Rhizobium species was not influenced by the presence of 40 or 80 mg/ml concentrations of extracts of shoots or rhizomes. Quackgrass may inhibit indirectly the legume-Rhizobium symbiosis by inhibiting root hair formation rather than directly inhibiting Rhizobium growth. The presence of soil microorganisms was not necessary for the development of quackgrass toxicity in soil or agar. Soil microorganisms reduced toxicity of quackgrass residues in soil.

Postemergence applications of BAS 9052 OH {2-[1-(ethoxyimino) butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexene-1-one} and Ro-13-8895 {acetone-O-[d-2-[p-[(α,α,α-trifluoro-p-tolyl-oxy] phenoxy] propionyl]oxime} at 0.3 kg/ha selectively controlled four- to five-leaf-stage quackgrass [Agropyron repens (L.) Beauv. ♯3 AGRRE] 75 and 80%, respectively, 105 days after treatment (DAT). KK-80 {4-[4-[4-(trifluoromethyl)phenoxy) phenoxy]-2-pentenoic} at the same rate and stage of growth controlled quackgrass only 45%. None of the herbicides had preemergence activity on quackgrass. All herbicides applied at 0.8 kg/ha controlled two- to three- or four- to five-leaf-stage quackgrass an average of 88%, whereas applications at the six- to seven-leaf stage controlled quackgrass only 27%, 105 DAT. Cultivation alone 1 DAT provided quackgrass control that gave soybean [Glycine max (L.) Merr.] yields equal to any herbicide or herbicide plus cultivation treatment In a greenhouse experiment, BAS 9052 OH killed 50-bud rhizome systems in 1 day, whereas KK-80 and Ro-13-8895 required 14 and 7 days of translocation, respectively, to provide similar control.

Experiments were conducted in growth chambers to evaluate the effect of soil temperature and soil moisture on the distribution of 14C-photoassimilates and 14C-glyphosate [N-(phosphonomethyl) glycine] in quackgrass [Agropyron repens (L.) Beauv. ♯3 AGRRE]. When 14C-glyphosate was applied to leaves, the radioactivity was less in the rhizome buds of plants exposed to 7-C soil temperature than in plants exposed to 12- and 18-C soil temperatures after 2 days. In plants with leaves exposed to 14CO2, the radioactivity from 14C-photoassimilates was greatest in rhizomes and rhizome buds of plants at the 12-C soil temperature. As soil moisture levels were decreased, uptake of C-glyphosate into leaves declined, and transport to the daughter shoots, rhizomes, and rhizome buds was reduced. The concentration of 14C-photoassimilates in the rhizome system of water-stressed quackgrass plants was similar to that in nonstressed plants. This study shows that the patterns of glyphosate distribution differ from those of photoassimilate distribution in quackgrass plants exposed to water stress.

The effect of site of application on uptake and translocation of the butyl ester of fluazifop {(±)-2-[4-[[5-(trifluoromethyl)-2-pyridinyl]oxy] phenoxy] propanoic acid} by quackgrass [Agropyron repens (L.) Beauv. # AGRRE] was investigated using 14C-labeled herbicide and intact plants. Uptake and distribution of the label were significantly greater from the abaxial than from the adaxial surface of leaves. The addition of a nonionic surfactant4 to the treatment solution increased the uptake significantly only through the adaxial surface. Uptake of 14C by the apical, middle, and basal regions of the treated leaf lamina did not differ significantly. However, movement of the 14C-label to stem areas and leaves both above and below treated leaves was greater from lamina base applications than from treatments to the lamina apex and middle. The older leaves absorbed more herbicide than did younger leaves, but the pattern of translocation did not differ. Considerably greater translocation occurred from treatments to the outside of the leaf sheaths in the lower regions of the stem than from applications to the upper leaf sheaths, with the 14C-label moved to young and old leaves, roots, and rhizomes. Uptake from applications to the outside of the upper leaf sheaths also resulted in improved translocation mainly within the stem areas and into upper leaves.

Studies were conducted to determine the effects of tillage level on weed control and yield of asparagus grown on a sandy loam soil under sprinkler irrigation. Without herbicides, horseweed and kochia populations increased in no-till asparagus production over a 3-yr period, compared to asparagus that was rototilled either once in the spring or once in the spring and again at layby. Without herbicides, hairy nightshade populations increased most when asparagus was rototilled both in the spring and at layby. Volunteer asparagus was controlled best when herbicides and tillage were combined in the spring and again at layby. Common groundsel was controlled without herbicides by rototilling both in the spring and at layby. Diuron at 1.4 kg ai/ha plus prodiamine at 1.4 kg/ha or metribuzin at 0.9 kg/ha plus norflurazon at 2 kg/ha controlled all weeds under all three tillage levels when split-applied in the spring and at layby. Tillage level had no effect on total asparagus spear number or weight if tillage was performed in mid March before asparagus emergence.

Quackgrass biotypes collected from 15 locations exhibited differential response to primisulfuron at 20 g ha−1 in field experiments at Hudson, NY during 1990 and 1991. Quackgrass control ranged from 23 to 100% 77 days after application (DAA) in 1990 and from 38 to 100% 88 DAA in 1991. The biotype from Montpelier, OH was the most tolerant both years. Differential response of quackgrass biotypes from five locations was confirmed in greenhouse experiments. The primisulfuron rate required to reduce shoot regrowth by 50% in the greenhouse was estimated at 15, 15, 17, 17, and 37 g ha−1 for biotypes from Amherst, MA; Mahnomen MN; Fargo, ND; State College, PA; and Montpelier, OH, respectively. Quackgrass shoot regrowth was reduced more when primisulfuron was applied with the linear alcohol ethoxylate additive 1412-70 compared with Scoil, Sylgard 309, Agridex, or X-77.

Field trials were carried out in 1989 and 1990 at St-Augustin, near Québec City, Canada, to determine the critical periods of quackgrass control in potato submitted to three levels of infestation. Potato yield losses due to quackgrass interference increased with quackgrass infestation and length of duration of interference. Quackgrass interference influenced marketable tuber yields more than total tuber yields. Duration of the critical period varied depending on the level of quackgrass infestation and year. Based on an arbitrary 5% level of marketable yield loss, the critical period started at ca. 15 days after emergence (DAE) of potato at low level of infestation, and at ca. 3 DAE at medium level of infestation. At high level of infestation, the critical period began prior to the emergence of potato. The end of the critical period of quackgrass removal was extremely variable across quackgrass infestation level and year and ranged from 23 to 68 DAE of potato at a 5 % yield loss level. It appears that onset of interference varied less than the end of it, indicating that early quackgrass control is necessary to prevent yield loss.

The phytotoxicity of the butyl ester of fluazifop to quackgrass was enhanced by the addition of a nonionic surfactant and an oil additive either alone or in mixture to the spray solution. The enhancement caused by the surfactant was consistently greater than that caused by the oil additive. A higher level of quackgrass control was achieved at the carrier volumes of 100, 200, or 400 L/ha, than at 800 L/ha. Quackgrass growth inhibition was greater following application of small herbicide droplets which averaged 0.25 μl compared to larger herbicide droplets at each herbicide application rate. When droplet concentration was varied and different doses of the herbicides applied to plants, no significant differences were noted at the highest dose. However, at intermediate dose treatments, the least concentrated droplets (2.5 μg/μl) were most phytotoxic. Herbicide droplets placed at basal areas of leaves were more phytotoxic than when placed at lamina apices and middle areas. This effect was most pronounced on adaxial rather than abaxial surfaces. In general, application of droplets to younger leaves resulted in greater phytotoxicity than treatment of older leaves.

The herbicides 2,4-D [(2,4-dichlorophenoxy) acetic acid], dalapon (2,2-dichloropropionic acid), glyphosate [(N-phosphonomethyl)glycine], paraquat (1,1’-dimethyl-4,4’-bypiridinium ion), and pronamide [3,5-dichloro-(N-1,1-dimethyl-2-propynyl)benzamide] were applied at selected rates and dates to quackgrass [Agropyron repens (L.) Beauv. ♯ AGRRE]-infested alfalfa (Medicago sativa L.) sod in 1979 and 1980 to suppress vegetation for subsequent establishment of alfalfa without tillage. The influence of herbicides on weed control, alfalfa seedling density and visual stand, and forage yield and quality was assessed for 3 yr. During establishment, all herbicides except 2,4-D effectively controlled quackgrass. Glyphosate applied broadcast increasingly suppressed the existing sod as the rate was increased from 0.6 to 1.6 kg ai/ha. The effectiveness of herbicides as measured by total (old and new) plant density was closely related to the suppression of the sward. While the substitution of new alfalfa plants for old ones was not necessary under dense (40 plants/m2) alfalfa stands, completely controlling the vegetation with glyphosate resulted in sustained productivity throughout the experimental period. After 3 yr, alfalfa percentage and yield were highest when glyphosate was broadcast at 1.6 kg/ha. Improvement in forage quality, as estimated by the concentrations of crude protein and neutral-detergent fiber, was closely related to grass control.