We report a novel technique for micromechanical detection of biologically relevant glucose by immobilization of glucose oxidase (GOx) onto a microcantilever surface. Microfabricated cantilevers have recently attracted considerable interest in the development of a wide range of novel physical, chemical, and biological sensors. This paper describes the combination of this novel technology with enzyme specificity to construct a highly selective glucose biosensor. The enzyme-functionalized microcantilever undergoes bending due to a change in surface stress induced by the reaction between glucose and the GOx immobilized on the cantilever surface. The common interferents for glucose detection in other detection schemes have been tested and have shown no effect on the measurement of blood glucose level by this technique.