Hostname: page-component-8448b6f56d-t5pn6 Total loading time: 0 Render date: 2024-04-24T03:33:59.832Z Has data issue: false hasContentIssue false
  • English
  • Français

Novel Glucose Biosensor Based on the Microcantilever

Published online by Cambridge University Press:  15 February 2011

Jianhong Pei ,
Fang Tian  and
Thomas Thundat
Jianhong Pei
Affiliation:
Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6123
Fang Tian
Affiliation:
Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6123
Thomas Thundat
Affiliation:
Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6123
Get access

Abstract

We report a novel technique for micromechanical detection of biologically relevant glucose by immobilization of glucose oxidase (GOx) onto a microcantilever surface. Microfabricated cantilevers have recently attracted considerable interest in the development of a wide range of novel physical, chemical, and biological sensors. This paper describes the combination of this novel technology with enzyme specificity to construct a highly selective glucose biosensor. The enzyme-functionalized microcantilever undergoes bending due to a change in surface stress induced by the reaction between glucose and the GOx immobilized on the cantilever surface. The common interferents for glucose detection in other detection schemes have been tested and have shown no effect on the measurement of blood glucose level by this technique.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 776: Symposium Q – Unconventional Approaches to Nanostructures with Applications in Electronics, Photonics, Information Storage and Sensing , 2003 , Q11.21
Copyright
Copyright © Materials Research Society 2003

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

1

Present address: Nova Biomedical Corporation, 200 Prospect Street Waltham, MA 02454

References

1. Thundat, T., Chen, G. Y., Warmack, R. J., Allison, D. P. and Wachter, E. A., Anal. Chem. 67, 519, (1996)Google Scholar
2. Chen, G. Y., Thundat, T., Wachter, E. A. and Warmack, R. J., J. Appl. Phys. 77, 3618, (1995)Google Scholar
3. Xu, X., Thundat, T., Brown, G. and Ji, H., Anal. Chem. 74, 3611, (2002)Google Scholar
4. Preissing, F. J., J. Appl. Phys, 66, 4262, 1989.Google Scholar
5. Hanson, K. M., Ji, H., Wu, G., Datar, R., Cote, R., Majumdar, A. and Thundat, T., Anal. Chem. 73, 1567, (2001)Google Scholar
6. Fritz, J., Baller, M. K., Lang, H. P., Rothuizen, H., Vettiger, P., Meyer, E., Güntherodt, H. J., Gerber, Ch. and Gimzewski, J. K., Science, 288, 316, (2000)Google Scholar
7. Wu, G., Datar, R., Hanson, K., Thundat, T., Cote, R. and Majumdar, A., Nature Biotechnology, 19, 856, (2001)Google Scholar
8. Stevenson, K., Mehta, A., Sachenko, P., Hanson, K. and Thundat, T., Langmuir, 18, 8732, (2002)Google Scholar
9. Wilson, G. S. and Hu, Y., Chem. Rev, 100, 2693, (2000)Google Scholar
10. Wang, J., Electroanal. 13, 983, (2001)Google Scholar