Book contents
- Frontmatter
- Contents
- Contributors
- Preface
- Acknowledgements
- Abbreviations and symbols
- Part I Chlorophylls and carotenoids
- Part II Methodology guidance
- 4 New HPLC separation techniques
- 5 The importance of a quality assurance plan for method validation and minimizing uncertainties in the HPLC analysis of phytoplankton pigments
- 6 Quantitative interpretation of chemotaxonomic pigment data
- 7 Liquid chromatography-mass spectrometry for pigment analysis
- 8 Multivariate analysis of extracted pigments using spectrophotometric and spectrofluorometric methods
- Part III Water-soluble ‘pigments’
- Part IV Selected pigment applications in oceanography
- Part V Future perspectives
- Part VI Aids for practical laboratory work
- Part VII Data sheets aiding identification of phytoplankton carotenoids and chlorophylls
- Index
- Plate Section
- References
4 - New HPLC separation techniques
Published online by Cambridge University Press: 05 March 2012
Book contents
- Frontmatter
- Contents
- Contributors
- Preface
- Acknowledgements
- Abbreviations and symbols
- Part I Chlorophylls and carotenoids
- Part II Methodology guidance
- 4 New HPLC separation techniques
- 5 The importance of a quality assurance plan for method validation and minimizing uncertainties in the HPLC analysis of phytoplankton pigments
- 6 Quantitative interpretation of chemotaxonomic pigment data
- 7 Liquid chromatography-mass spectrometry for pigment analysis
- 8 Multivariate analysis of extracted pigments using spectrophotometric and spectrofluorometric methods
- Part III Water-soluble ‘pigments’
- Part IV Selected pigment applications in oceanography
- Part V Future perspectives
- Part VI Aids for practical laboratory work
- Part VII Data sheets aiding identification of phytoplankton carotenoids and chlorophylls
- Index
- Plate Section
- References
Summary
A summary is not available for this content so a preview has been provided. Please use the Get access link above for information on how to access this content.
- Type
- Chapter
- Information
- Phytoplankton PigmentsCharacterization, Chemotaxonomy and Applications in Oceanography, pp. 165 - 194Publisher: Cambridge University PressPrint publication year: 2011
References
2006 Environmental forcing of phytoplankton floral composition, biomass and primary productivity in Chesapeake Bay, USAEstuar. Coast. Shelf Sci 67 108CrossRefGoogle Scholar
1985 Characterization of silica-based reversed-phase columns with respect to retention selectivity. Solvophobic effectsJ. Chromatogr 321 1CrossRefGoogle Scholar
2006 Improved detection and characterization of fucoxanthin-type carotenoids: novel pigments in (Prymnesiophyceae)J. Phycol 42 391CrossRefGoogle Scholar
2001 Development and application of a high resolution liquid chromatography method for the analysis of complex pigment distributionsJ. Chromatogr. A 917 167CrossRefGoogle ScholarPubMed
2001 Identification of the bacteriochlorophyll homologues of strain UdG6053 grown at low light intensityPhotosynth. Res 70 221CrossRefGoogle ScholarPubMed
1997 Improved resolution of mono-and divinyl chlorophylls and and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLCMar. Ecol. Prog. Ser 161 303CrossRefGoogle Scholar
2004 Pigment adaptations in surface phytoplankton along the eastern boundary of the Atlantic OceanMar. Ecol. Prog. Ser 281 13CrossRefGoogle Scholar
2005 An obligately photosynthetic bacterial anaerobe from a deep-sea hydrothermal vent. Sci. USA 102 9306CrossRefGoogle ScholarPubMed
2007 Separation of chlorines and carotenoids in capillary electrophoresisJ. Chromatogr. Sci 45 593CrossRefGoogle Scholar
1996 Plant Pigments as Biomarkers of Organic Matter Sources in Sediments and Coastal Waters of Cyprus (eastern Mediterranean)Estuar. Coast. Shelf Sci 42 103CrossRefGoogle Scholar
2000 Breakdown of phytoplankton pigments in Baltic sediments: effects of anoxia and loss of deposit-feeding macrofaunaJ. Exp. Mar. Biol. Ecol 251 161CrossRefGoogle ScholarPubMed
2005 Analysis of algal pigments by high performance liquid chromatographyAlgal Culturing TechniquesBurlingtonElsevier Academic Press327Google Scholar
1997 Structural relationships between algal carotenoidsPhytoplankton Pigments in Oceanography: Guidelines to Modern MethodsParisUNESCO Publishing572Google Scholar
2005 Benthic and planktonic algal communities in a high artic lake: pigment structure and contrasting responses to nutrient enrichmentJ. Phycol 41 1120CrossRefGoogle Scholar
1994 Separation of bacteriochlorophyll homologues from green photosynthetic sulphur bacteria by reversed-phase HPLCPhotosynth. Res 41 157CrossRefGoogle ScholarPubMed
1995 Rearrangement of light harvesting bacteriochlorophyll homologues as a response of green sulphur bacteria to low light intensitiesPhotosynth. Res 45 21CrossRefGoogle ScholarPubMed
2007 Microphytobenthic biomass assessment by pigment analysis: comparison of spectrophotometry and high performance liquid chromatography methodsHydrobiologia 587 19CrossRefGoogle Scholar
2003 The use of HPLC pigment analysis to study microphytobenthos communitiesActa Oecol 24 S109CrossRefGoogle Scholar
2007 Factors influencing the variability of pigments in the surface sediments of mountain lakesFreshwat. Biol 52 1365CrossRefGoogle Scholar
2003 Characterization of spring phytoplankton communities in the Rio de la Plata maritime front using pigment signatures and cell microscopyMar. Biol 143 1013CrossRefGoogle Scholar
2006 Comparative study on microphytobenthic pigments of muddy and sandy intertidal sediments of the Tagus estuaryEstuar. Coast. Shelf Sci 66 225CrossRefGoogle Scholar
2001 Historical trends of hypoxia on the Louisiana shelf: application of pigments as biomarkersOrg. Geochem 32 543CrossRefGoogle Scholar
1992 nov. gen. nov. sp.: an oxyphototrophic marine prokaryote containing divinyl chlorophyll and . Microbiol 157 297Google Scholar
2004 An intercomparison of HPLC phytoplankton pigment methods using in situ samples: application to remote sensing and database activitiesMar. Chem 85 41CrossRefGoogle Scholar
2006 Aerobic anoxygenic phototrophic bacteria in the Mid-Atlantic Bight and the North Pacific GyreAppl. Environ. Microbiol 72 557CrossRefGoogle ScholarPubMed
2002 Trace metal/phytoplankton interactions in the SkagerrakJ. Marine Syst 35 39CrossRefGoogle Scholar
2006 Temporal variability in phytoplankton pigments, picoplankton and coccolithophores along a transect through the North Atlantic and tropical southwestern PacificDeep-Sea Res. I 53 689CrossRefGoogle Scholar
2003 gen. nov. (Gymnodiniales, Dinophyceae), a new genus of unarmoured dinoflagellates with sigmoid apical grooves, including the description of two new speciesJ. Phycol 39 1233CrossRefGoogle Scholar
2005 sp. nov. (Gymnodiniales, Dinophyceae), a new potentially ichthyotoxic unarmoured dinoflagellate from lagoonal habitats of south-east AustraliaPhycologia 44 640CrossRefGoogle Scholar
2003 Phytoplankton assemblage structure and primary productivity along 170°W in the South Pacific OceanMar. Ecol. Prog. Ser 255 55CrossRefGoogle Scholar
2005 Influence of iron on algal community composition and physiological status in the Peru upwelling systemLimnol. Oceanogr 50 1887CrossRefGoogle Scholar
2002 Temperature selectivity in reversed-phase high-performance liquid chromatographyJ. Chromatogr. A 965 195CrossRefGoogle ScholarPubMed
1998 Simultaneous variation of temperature and gradient steepness for reversed-phase high-performance liquid chromatography method development, I. Application to 14 different samples using computer simulationJ. Chromatogr. A 803 1CrossRefGoogle ScholarPubMed
1998 Simultaneous variation of temperature and gradient steepness for reversed-phase high-performance liquid chromatography method development, II. The use of further changes in conditionsJ. Chromatogr. A 803 33CrossRefGoogle ScholarPubMed
2000 Selectivity differences for C18 and C8 reversed-phase columns as a function of temperature and gradient steepness: I. Optimizing selectivity and resolutionJ. Chromatogr. A 897 37CrossRefGoogle Scholar
2000 Benthic-pelagic coupling in the oligotrophic Cretan seaProgr. Oceanogr 46 457CrossRefGoogle Scholar
2004 gen. and sp. nov. (Dictyochophyceae, Heterokontophyta) a small flagellate isolated from the English ChannelPhycologia 43 658CrossRefGoogle Scholar
2000 Algal carotenoids. Part 64. Structure and chemistry of 4-keto-19’-hexanoyloxyfucoxanthin with a novel carotenoid end groupJ. Chem. Soc., Perkin Trans. 1 2000 1223CrossRefGoogle Scholar
2008 Separation efficiency of particle-packed HPLC microchipsAnal. Chem 80 5945CrossRefGoogle ScholarPubMed
2004 Diversity of planktonic photoautotrophic microorganisms along a salinity gradient as depicted by microscopy, flow-cytometry, pigment analysis and DNA-based methodsFEMS Microbiol. Ecol 49 281CrossRefGoogle ScholarPubMed
1989 A new form of chlorophyll involved in light-harvestingPlant Physiol 91 727CrossRefGoogle ScholarPubMed
1988 Pigment preservation and remineralization in oxic coastal marine sedimentsGeochim. Cosmochim. Acta 52 87CrossRefGoogle Scholar
2007 Environmental drivers of phytoplankton distribution and composition in Tagus Estuary, PortugalEstuar. Coast. Shelf Sci 75 21CrossRefGoogle Scholar
1993 High performance liquid chromatography of chlorophylls 3, 1, 2 and and of carotenoids of chromophyte algae on a polymeric octadecyl silica columnChromatographia 35 543CrossRefGoogle Scholar
1993 High performance liquid chromatographic separation of polar and non-polar chlorophyll pigments from algae using a wide pore polymeric octadecylsilica columnJ. High Resol. Chromatogr 16 229CrossRefGoogle Scholar
1996 Ion-pair reversed phase high-performance liquid chromatography of algal chlorophyllsJ. Chromatogr. A 738 285CrossRefGoogle Scholar
1997 Reversed phase high performance liquid chromatographic separation of mono- and divinyl chlorophyll forms using pyridine-containing mobile phases and a polymeric octadecyl silica columnChromatographia 44 43CrossRefGoogle Scholar
1998 Detection of new pigments from (Prymnesiophyceae) by high-performance liquid chromatography, liquid chromatography-mass spectrometry, visible spectroscopy and fast atom bombardment mass spectrometryJ. Phycol 34 70CrossRefGoogle Scholar
2006 Chlorophyll analysis by new HPLC methodsChlorophylls and Bacteriochlorophylls: Biochemistry, Biophysics, Functions and ApplicationsDordrechtSpringer109CrossRefGoogle Scholar
1995 Spectral characterization of new chlorophyll pigments isolated from (Prymnesiophyceae) by high-performance liquid chromatographyJ. Phycol 31 761CrossRefGoogle Scholar
2000 The main non-polar chlorophyll from (Prymnesiophyceae) is a chlorophyll 2-monogalactosyldiacylglyceride ester: a mass spectrometry studyJ. Phycol 36 497CrossRefGoogle Scholar
2003 Rapid separation of chlorophyll and and their demetallated and dephytylated derivatives using a monolithic silica C18 column and a pyridine-containing mobile phaseJ. Chromatogr. A 994 85CrossRefGoogle Scholar
2001 Phytoplankton pigment chemotaxonomy of the northeastern AtlanticDeep-Sea Res. II 48 795CrossRefGoogle Scholar
2003 Characterization of the chlorosome antenna of the filamentous anoxygenic phototrophic bacterium sp. strain UdG9001Arch. Microbiol 180 417CrossRefGoogle ScholarPubMed
2002 Novel bacteriochlorophyll structures and species-specific variability of pigment composition in green sulfur bacteriaArch. Microbiol 177 475CrossRefGoogle ScholarPubMed
2002 Bacteriochlorophyll in the ocean: Is anoxygenic photosynthesis important?Limnol. Oceanogr 47 290CrossRefGoogle Scholar
1993 Chlorophylls and and divinyl chlorophylls and in the open Subtropical North Atlantic OceanMar. Ecol. Prog. Ser 101 307CrossRefGoogle Scholar
2000 A novel niche for sp. in low-light suboxic environments in the Arabian Sea and the Eastern Tropical North PacificDeep-Sea Res 47 1183CrossRefGoogle Scholar
2000 Distribution and sources of cyclic pheophorbides in the marine environmentLimnol. Oceanogr 45 200CrossRefGoogle Scholar
2007 Accurately measuring the abundance of benthic microalgae in spatially variable habitatsLimnol. Oceanogr. Methods 5 119CrossRefGoogle Scholar
2004 Routine quantification of phytoplankton groups – microscopy or pigment analyses?Mar. Ecol. Prog. Ser 273 31CrossRefGoogle Scholar
2007 Recent applications in nanoliquid chromatographyJ. Sep. Sci 30 1589CrossRefGoogle ScholarPubMed
2000 The First SeaWiFS HPLC analysis round-robin experiment (SeaHARRE-1) Vol. 14
2006 Improved high performance liquid chromatographic method for determination of carotenoids in the microalga J. Chromatogr. A 1102 193CrossRefGoogle ScholarPubMed
2006 Polarity parameters of the Symmetry C18 and Chromolith Performance RP-18 monolithic chromatographic columnsJ. Chromatogr. A 1107 96CrossRefGoogle ScholarPubMed
1997 Application of pigment methods in OceanographyPhytoplankton Pigments in Oceanography: Guidelines to Modern MethodsParisUNESCO Publishing127Google Scholar
1997 Structural relationships between algal chlorophyllsPhytoplankton Pigments in Oceanography: Guidelines to Modern MethodsParisUNESCO Publishing566Google Scholar
1997 Minimum criteria for identifying phytoplankton pigmentsPhytoplankton Pigments in Oceanography: Guidelines to Modern MethodsParisUNESCO Publishing631Google Scholar
1999 Recent advances in HPLC pigment analysis of phytoplanktonMar. Freshwat. Res 50 879CrossRefGoogle Scholar
2006 Effect of sediment type on microphytobenthos vertical distribution: Modelling the productive biomass and improving ground truth measurementsJ. Exp. Mar. Biol. Ecol 332 60CrossRefGoogle Scholar
1995 Separation of carotenoids by high-performance liquid chromatography with polymeric and monomeric octadecylsilica stationary phasesChromatographia 41 311CrossRefGoogle Scholar
1994 Protocols for the Joint Global Ocean Flux Study Core MeasurementsSCOR, Manual and Guides, UNESCO 29 91Google Scholar
2006 Geochemistry of chlorophyllsChlorophylls and Bacteriochlorophylls. Biochemistry, Biophysics, Functions and ApplicationsDordrechtSpringer535CrossRefGoogle Scholar
2007 Rapid growth rates of aerobic anoxygenic phototrophs in the oceanEnviron. Microbiol 9 2401CrossRefGoogle ScholarPubMed
1992 Separation of chlorophylls 1, 2 and 3 of marine phytoplankton by reversed-phase C18 high–performance liquid chromatographyJ. Phycol 28 708CrossRefGoogle Scholar
1998 A comparison of phytoplankton populations of the Arabian Sea during the spring intermonsoon and southwest monsoon of 1995 as described by HPLC-analyzed pigmentsDeep-Sea Res. II 45 2133CrossRefGoogle Scholar
2005 Molecular dynamics simulations of alkylsilane stationary-phase order and disorder. 1. Effects of surface coverage and bonding chemistryAnal. Chem 77 7852CrossRefGoogle ScholarPubMed
2005 Molecular dynamics simulations of alkylsilane stationary-phase order and disorder. 2. Effects of temperature and chain lengthAnal. Chem 77 7862CrossRefGoogle ScholarPubMed
2005 Phytoplankton community assemblage in the English Channel: a comparison using chl derived from HPLC-CHEMTAX and carbon derived from microscopy cell countsJ. Plankton Res 27 103CrossRefGoogle Scholar
1998 Chlorophyll degradation during cellular senescence and deathOrg. Geochem 29 1233CrossRefGoogle Scholar
2000 Early diagenetic alteration of chlorophyll and bacteriochlorophyll in a contemporaneous marine ecosystem: Florida BayOrg. Geochem 31 1561CrossRefGoogle Scholar
2002 Senescence- and death-related alteration of chlorophylls and carotenoids in marine phytoplanktonOrg. Geochem 33 1635CrossRefGoogle Scholar
2007 Distribution, phylogeny, and growth of cold-adapted picoprasinophytes in Arctic seasJ. Phycol 43 78CrossRefGoogle Scholar
2000 Benthic-pelagic exchange of microalgae at a tidal flat. 1. Pigment analysisMar. Ecol. Prog. Ser 196 59CrossRefGoogle Scholar
1983 The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatographyAnal. Chim. Acta 151 297CrossRefGoogle Scholar
1997 Simple isocratic HPLC methods for chlorophyll and their degradation productsPhytoplankton Pigments in Oceanography: Guidelines to Modern MethodsParisUNESCO Publishing307Google Scholar
2007 Role of advances in chromatographic techniques in phytochemistryPhytochem 68 2785CrossRefGoogle ScholarPubMed
2004 Variations in the distributions of sedimentary alkyl porphyrins in the Mulhouse basin in response to changing environmental conditionsOrg. Geochem 35 1229CrossRefGoogle Scholar
1997 Pigment composition of a novel photosynthetic prokaryote containing chlorophyll as the major chlorophyllPlant Cell Physiol 38 272CrossRefGoogle Scholar
2007 Differences in photosynthetic pigment signatures between phytoneuston and phytoplankton communities in a coastal lagoon of Baja CaliforniaMar. Biol 151 1225CrossRefGoogle Scholar
2006 Spatial variation in phytoplankton dynamics in the Belgian coastal zone of the North Sea studied by microscopy, HPLC-CHEMTAX and underway fluorescence recordingsJ. Sea Res 55 253CrossRefGoogle Scholar
2006 Fast analysis in liquid chromatography using small particle size and high pressureJ. Sep. Sci 29 1836CrossRefGoogle ScholarPubMed
2004 A single species, (Prasinophyceae), dominates the eukaryotic picoplankton in the western English ChannelAppl. Environ. Microbiol 70 4064CrossRefGoogle Scholar
2005 Late summer community composition and abundance of photosynthetic picoeukaryotes in Norwegian and Barents seasLimnol. Oceanogr 50 1677CrossRefGoogle Scholar
1998 Microbiological mediation of spectral reflectance from intertidal cohesive sedimentsLimnol. Oceanogr 43 1207CrossRefGoogle Scholar
1978 General physiology and ecology of photosynthetic bacteriaThe Photosynthetic BacteriaNew YorkPlenum Press3Google Scholar
1996 Flow scintillation counting of 14C labeled microalgal photosynthetic pigmentsJ. Plankton Res 18 1867CrossRefGoogle Scholar
2003 Microalgal-meiofaunal trophic relationships in muddy intertidal sedimentsAquat. Microb. Ecol 31 99CrossRefGoogle Scholar
1999 Chromatography and electrophoresis on chips: critical elements of future integrated, microfluidic analytical systems for life scienceTrends Biotechnol 17 101CrossRefGoogle Scholar
2005 Preservation conditions and the use of sediment pigments as a tool for recent ecological reconstruction in four Northern European estuariesMar. Chem 95 283CrossRefGoogle Scholar
2001 Phytoplankton community structure derived from HPLC analysis of pigments in the Faroe-Shetland Channel during summer 1999: the distribution of taxonomic groups in relation to physical/chemical conditions in the photic zoneJ. Plankton Res 23 191CrossRefGoogle Scholar
2006 Size fractionated phytoplankton pigment groups in the NW Iberian upwelling system: impact of the Iberian poleward currentMar. Ecol. Prog. Ser 323 59CrossRefGoogle Scholar
2003 Temporal variation in phytoplankton assemblages and pigment composition at a fixed station of the Ría of Pontevedra (NW Spain)Estuar. Coast. Shelf Sci 58 499CrossRefGoogle Scholar
2002 Phytoplankton assemblages in Gerlache and Bransfield Straits (Antarctic Peninsula) determined by light microscopy and CHEMTAX analysis of HPLC pigment dataDeep-Sea Research II 49 723CrossRefGoogle Scholar
1998 High performance liquid chromatographic separation of chlorophyll forms from marine phytoplankton on octylsilica bonded phasesChromatographia 48 677CrossRefGoogle Scholar
2006 Symposium-in-Print: UV effects on aquatic and coastal ecosystems. Effects of enhanced UV-B on pigment-based phytoplankton biomass and composition of mesocosm-enclosed natural marine communities from three latitudesPhotochem. Photobiol 82 909CrossRefGoogle Scholar
2001 The influence of column temperature on selectivity in reversed-phase liquid chromatography for shape constrained solutesJ. Sep. Sci 24 9103.0.CO;2-A>CrossRefGoogle Scholar
1994 Development of engineered stationary phases for the separation of carotenoid isomersAnal. Chem 66 1667CrossRefGoogle ScholarPubMed
1999 Shape selectivity for constrained solutes in reversed-phase liquid chromatographyAnal. Chem 71 4821CrossRefGoogle ScholarPubMed
2000 C30 Stationary phases for the analysis of food by liquid chromatographyJ. Chromatogr. A 880 189CrossRefGoogle ScholarPubMed
1993 Separation of chlorophyll 1 and 2 by reversed-phase high-performance liquid chromatographyJ. Chromatogr. A 653 247CrossRefGoogle Scholar
1995 Determination of chlorophyll by reversed-phase high-performance liquid chromatography with isocratic elution and the column switching techniqueJ. Chromatogr. A 693 176CrossRefGoogle Scholar
2003 Detecting presence of phytoplankton groups with non-specific pigment signaturesJ. Appl. Phycol 15 465CrossRefGoogle Scholar
1995 HPLC-analysis of algal pigments: comparison of columns, column properties and eluentsJ. Appl. Phycol 7 487CrossRefGoogle Scholar
2005 Phytoplankton assemblages and their dominant pigments in the Nervion River estuaryHydrobiologia 549 1CrossRefGoogle Scholar
2005 Non-photochemical quenching of chlorophyll fluorescence and operation of the xanthophyll cycle in estuarine microphytobenthosJ. Exp. Mar. Biol. Ecol 326 157CrossRefGoogle Scholar
2005 Evidence of late quaternary environmental change in a continental east Antarctic lake from lacustrine sedimentary pigment distributionsAntarct. Sci 17 361CrossRefGoogle Scholar
2002 A study of the critical criteria for analyte stability in high-temperature liquid chromatographyAnal. Chem 74 1017CrossRefGoogle ScholarPubMed
2004 HPLC pigment records provide evidence of past blooms of in the coastal bays of Maryland and Virginia, USAHarmful Algae 3 295CrossRefGoogle Scholar
2001 Computer-assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigmentsJ. Chromatogr. A 910 31CrossRefGoogle ScholarPubMed
1992 High-performance liquid chromatography of phytoplankton pigments using a polymeric reversed phase C18 columnJ. Phycol 29 867CrossRefGoogle Scholar
1994 Improved separations of phytoplankton pigments using temperature-controlled high-performance liquid chromatographyMar. Ecol. Prog. Ser 114 303CrossRefGoogle Scholar
1998 Pigment distribution in the Pacific region of the Southern Ocean (Autumn 1995)Polar Biol 19 348CrossRefGoogle Scholar
1995 Temperature-programmed high-performance liquid chromatography separation of mono- and divinyl chlorophyll forms from marine phytoplanktonChromatographia 41 539CrossRefGoogle Scholar
2003 Pigment signatures and phylogenetic relationships of the Pavlovophyceae (Haptophyta)J. Phycol 39 379CrossRefGoogle Scholar
2004 Phytoplankton in the subtropical Atlantic Ocean: towards a better assessment of biomass and compositionDeep-Sea Res. I 51 507CrossRefGoogle Scholar
1996 Determination of chlorophylls and carotenoids of marine phytoplankton: separation of chlorophyll from divinyl chlorophyll and zeaxanthin from luteinJ. Plankton Res 18 2377CrossRefGoogle Scholar
2004 Structural variations in derivatives of the bacteriochlorophylls of Chlorobiaceae: impact of stratigraphic resolution on depth profiles as revealed by methanolysisOrg. Geochem 35 1299CrossRefGoogle Scholar
1994 Pigment patterns in suspended matter from the Elbe estuary, Northern GermanyNeth. J. Aquat. Ecol 28 255CrossRefGoogle Scholar
1996 Pigments in suspended matter from the Elbe estuary and the German Bight. Their use as marker compounds for the characterisation of suspended matter and in the interpretation of heavy metal loadingsArch. Hydrobiol. Spec. Issues Adv. Limnol 47 53Google Scholar
2000 Control of column temperature in reversed-phase liquid chromatographyJ. Chromatogr. A 869 211CrossRefGoogle ScholarPubMed
1997 High-resolution HPLC system for chlorophylls and carotenoids of marine phytoplanktonPhytoplankton Pigments in Oceanography: Guidelines to Modern MethodsParisUNESCO Publishing327Google Scholar
2006 Pigment markers for phytoplankton productionMarine Organic Matter: Biomarkers, Isotopes and DNABerlinSpringer71CrossRefGoogle Scholar
1997 Guidelines for selecting and setting up an HPLC system and laboratoryPhytoplankton Pigments in Oceanography: Guidelines to Modern MethodsParisUNESCO Publishing383Google Scholar
2000 Phytoplankton community structure and stocks in the East Antarctic marginal ice zone (BROKE survey, January–March 1996) pigment signaturesDeep-Sea Res. II 47 2363CrossRefGoogle Scholar
1991 Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplanktonMar. Ecol. Prog. Ser 77 183CrossRefGoogle Scholar
2006 Spatial variability in the coupling of organic carbon, nutrients, and phytoplankton pigments in surface waters and sediments of the Mississippi River plumeEstuar. Coast. Shelf Sci 69 47CrossRefGoogle Scholar
2007 Prasinoxanthin-containing Prasinophyceae discovered in Jiaozhou Bay, ChinaJ. Integr. Plant Biol 49 497CrossRefGoogle Scholar
2005 Recent advances in pigment analysis as applied to picophytoplanktonVie et Milieu 55 233Google Scholar
2000 Separation of chlorophylls and carotenoids from marine phytoplankton: A new HPLC method using a reversed-phase C8 column and pyridine-containing mobile phasesMar. Ecol. Prog. Ser 195 29CrossRefGoogle Scholar
2001 Chlorophyll 2 monogalactosyldiacylglyceride ester (chl 2-MGDG). A novel marker pigment for C species (Haptophyta)Mar. Ecol. Prog. Ser 219 85CrossRefGoogle Scholar
2004 Photosynthetic pigments in 37 species (65 strains) of Haptophyta: implications for oceanography and chemotaxonomyMar. Ecol. Prog. Ser 270 83CrossRefGoogle Scholar
1996 Combined use of temperature and solvent strength in reversed phase gradient elution, II. Comparing selectivity for different samples and systemsJ. Chromatogr. A 756 41CrossRefGoogle Scholar
2005 Elevated temperature HPLC: principles and applications to small molecules and biomoleculesLCGC North America 23 54Google Scholar
1996 Combined use of temperature and solvent strength in reversed-phase gradient elution, I. Predicting separation as a function of temperature and gradient conditionsJ. Chromatogr. A 756 21CrossRefGoogle ScholarPubMed
- 9
- Cited by