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2 - The Application of Isothermal Titration Calorimetry for Investigating Proton and Metal Interactions on Microbial Surfaces

from Part II - Advanced Analytical Instrumentation

Published online by Cambridge University Press:  06 July 2019

Janice P. L. Kenney
Affiliation:
MacEwan University, Edmonton
Harish Veeramani
Affiliation:
Carleton University, Ottawa
Daniel S. Alessi
Affiliation:
University of Alberta
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Summary

Isothermal titration calorimetry combined with surface complexation modeling is an ideal technique to provide further characterization of microbial surface reactivity towards protons and metal ions. This technique can produce enthalpies of protonation and metal ion coordination of acidic functional groups on microbial surfaces. This information is critical for understanding the thermodynamic driving force of surface complexation and provides key information for the indirect identification of surface ligands. Topics covered in this chapter include how this technique complements traditional methods of microbial surface reactivity, necessary system characterization prior to performing calorimetric experiments, how to prepare biomass and solutions for calorimetric titrations, difficult aspects of this technique, and data analysis and interpretation.

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Chapter
Information
Analytical Geomicrobiology
A Handbook of Instrumental Techniques
, pp. 63 - 78
Publisher: Cambridge University Press
Print publication year: 2019

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References

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