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Resistance patterns and molecular basis to ACCase-inhibiting herbicides

Published online by Cambridge University Press:  08 May 2024

Qian Yang Open the ORCID record for Qian Yang [Opens in a new window] ,
Wei Deng Open the ORCID record for Wei Deng [Opens in a new window] ,
Longwei Liu ,
Tian Wei ,
Xia Yang ,
Jinlei Zhu ,
Min Lv  and
Yongfeng Li Open the ORCID record for Yongfeng Li [Opens in a new window]
Qian Yang
Affiliation:
Associate Research Fellow, Jiangsu Lixiahe District Institute of Agricultural Sciences, Yangzhou, PR China
Wei Deng
Affiliation:
Associate Professor, College of Plant Protection, Yangzhou University, Yangzhou, PR China
Longwei Liu
Affiliation:
Undergraduate, College of Plant Protection, Yangzhou University, Yangzhou, PR China
Tian Wei
Affiliation:
Research Associate, Jiangsu Lixiahe District Institute of Agricultural Sciences, Yangzhou, PR China
Xia Yang
Affiliation:
Research Fellow, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, PR China
Jinlei Zhu
Affiliation:
Research Associate, Jiangsu Lixiahe District Institute of Agricultural Sciences, Yangzhou, PR China
Min Lv*
Affiliation:
Associate Research Fellow, Jiangsu Lixiahe District Institute of Agricultural Sciences, Yangzhou, PR China
Yongfeng Li*
Affiliation:
Research Fellow, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, PR China
*
Corresponding authors: Min Lv; Email: lvmin8889@126.com and Yongfeng Li; Email: liyongfeng_2020@aliyun.com
Corresponding authors: Min Lv; Email: lvmin8889@126.com and Yongfeng Li; Email: liyongfeng_2020@aliyun.com
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Abstract

Digitaria ciliaris var. chrysoblephara (Fig. & De Not.) R.R. Stewart is an annual xeromorphic weed that severely infests direct-seeded rice fields in China. Herbicide resistance is emerging in D. ciliaris var. chrysoblephara owing to extensive and recurrent use of the acetyl-CoA carboxylase (ACCase)-inhibiting herbicide metamifop. In this study, a total of 53 D. ciliaris var. chrysoblephara populations randomly sampled from direct-seeded rice fields across Jiangsu Province were investigated for metamifop resistance and potential resistance-endowing mutations. Single-dose assays revealed that 17 (32.1%) populations evolved resistance to metamifop and 5 (9.4%) populations were in the process of developing resistance. The resistance index (RI) of metamifop-resistant populations ranged from 2.7 to 32.1. Amino acid substitutions (Ile-1781-Leu, Trp-2027-Cys/Ser, and Ile-2041-Asn) in ACCase genes were detected in resistant D. ciliaris var. chrysoblephara plants and caused various cross-resistance patterns to ACCase-inhibiting herbicides. All of four resistant populations (YC07, YZ09, SQ03, and HA06), with different ACCase mutations, exhibited cross-resistance to the aryloxyphenoxypropionate (APP) herbicides cyhalofop-butyl (RI values: 10.0 to 19.9), fenoxaprop-P-ethyl (RI values: 53.7 to 132.8), and haloxyfop-P-methyl (RI values: 6.2 to 62.6), and the phenylpyrazoline (DEN) pinoxaden (RI values: 2.3 to 5.4), but responded differently to the cyclohexanedione (CHD) herbicides clethodim and sethoxydim. It is noteworthy that four postemergence herbicides used for rice cropping, including bispyribac-sodium, pyraclonil, quinclorac, and anilofos, showed poor control effect against D. ciliaris var. chrysoblephara, suggesting few alternations for managing this weed in rice fields except ACCase inhibitors. In conclusion, this work demonstrated that the D. ciliaris var. chrysoblephara had developed resistance to ACCase-inhibiting herbicides in rice cultivation of China, and target-site amino acid substitutions in ACCase were primarily responsible for metamifop resistance.

Keywords

ACCase gene mutationcross-resistancemetamifoptarget site–based resistance
Type
Research Article
Information
Weed Science , First View , pp. 1 - 8
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Copyright
© The Author(s), 2024. Published by Cambridge University Press on behalf of Weed Science Society of America

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Footnotes

Associate Editor: Gulshan Mahajan, Punjab Agricultural University

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