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Occurrence, Concentration, and Toxicity of Pyrrolizidine Alkaloids in Crotalaria Seeds

Published online by Cambridge University Press:  12 June 2017

M. Coburn Williams  and
Russell J. Molyneux
M. Coburn Williams
Affiliation:
U.S. Dep. Agric., Agric. Res. Serv., Logan, UT 84322
Russell J. Molyneux
Affiliation:
U.S. Dep. Agric., Agric. Res. Serv., Berkeley, CA 94710
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Abstract

Seed of 41 accessions of Crotalaria representing 35 species were analyzed for the concentration of total pyrrolizidine alkaloids (PAs) and for oral toxicity to 1-week-old chicks. All accessions were assayed for the presence of monocrotaline and spectabiline by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). High concentrations of PA were found in the seed of Crotalaria spectabilis Roth # CVTSP (3.85%), C. retusa L. (2.69%), C. alata Leveille (1.60%), C. quinquefolia L. (1.19%), and C. argyrolobioides Bak. (1.01%). The seed of Crotalaria argyrolobioides produced toxic signs but no deaths when fed at 10 mg/g of body weight, whereas the seed of the other four species were 100% lethal when fed to chicks in one dose at 2 to 6 mg/g of body weight. Seed of other species contained less than 0.6% PA and were nontoxic to chicks fed one dose at 10 mg/g of body weight. Monocrotaline was identified in 17 accessions and spectabiline in 7. No species contained both monocrotaline and spectabiline.

Keywords

Fabaceaehepatotoxinstoxic seed
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 35 , Issue 4 , July 1987 , pp. 476 - 481
Copyright
Copyright © 1987 by the Weed Science Society of America 

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References

Literature Cited

1. Bierer, B. W., Vickers, C. L., Rhodes, W. H., and Thomas, J. B. 1960. Comparison of the toxic effects of Crotalaria spectabilis and Crotalaria giant striata as complete feed contaminants. J. Am. Vet. Med. Assoc. 136:318–322.Google ScholarPubMed
2. Bull, L. B., Culvenor, C.C.J., and Dick, A. T. 1968. The pyrrolizidine alkaloids. North-Holland Publishing Co., Amsterdam. 293 pp.Google Scholar
3. Duke, J. A. 1981. Pages 61–70 in Handbook of Legumes of World Economic Importance. Plenum Press, New York.Google Scholar
4. Emmel, M. W. 1937. The toxicity of Crotalaria retusa L. seeds for the domestic fowl. J. Am. Vet. Med. Assoc. 91:205–206.Google Scholar
5. Emmel, M. W. 1948. Crotalaria poisoning in cattle. J. Am. Vet. Med. Assoc. 113:164.Google Scholar
6. Everist, S. L. 1981. Pages 412–415 in Poisonous Plants of Australia. 2nd ed. Angus & Robertson Pub., Sydney, Australia.Google Scholar
7. Johnson, A. E., Molyneux, R. J., and Merrill, G. B. 1985. Chemistry of toxic range plants. Variation in pyrrolizidine alkaloid content of Senecio, Amsinckia, and Crotalaria species. J. Agric. Food Chem. 33:50–55.Google Scholar
8. Kelly, J. W., Barber, C. W., Pate, D. D., and Hill, C. H. 1961. Effect of feeding Crotalaria seed to young chickens. J. Am. Vet. Med. Assoc. 139:1215–1217.Google Scholar
9. Kingsbury, J. M. 1964. Pages 314–320 in Poisonous Plants of the United States and Canada. Prentice-Hall, Inc., Englewood Cliffs, NJ.Google Scholar
10. Molyneux, R. J., Johnson, A. E., Roitman, J. N., and Benson, M. E. 1979. Chemistry of toxic range plants. Determination of pyrrolizidine alkaloid content and composition in Senecio species by nuclear magnetic reasonance spectroscopy. J. Agric. Food Chem. 27:494–499.Google Scholar
11. Molyneux, R. J. and Roitman, J. N. 1980. Specific detection of pyrrolizidine alkaloids on thin-layer chromatograms. J. Chromatogr. 195:412–415.Google Scholar
12. Peterson, J. E. and Culvenor, C.C.J. 1983. Heptotoxic pyrrolizidine alkaloids. Page 643 in Keeler, R. F. and Tu, A. T., eds. Handbook of Natural Toxins. Vol. 1. Marcel-Dekker, Inc., New York.Google Scholar
13. Pilbeam, D. J., Lyon-Joyce, A. J., and Bell, E. A. 1983. Occurrence of the pyrrolizidine alkaloid monocrotaline in Crotalaria seeds. J. Nat. Prod. 46:601–605.CrossRefGoogle Scholar
14. Ritchey, G. E., McKee, R., Becker, R. B., Neal, W. M., and Arnold, P.D.D. 1941. Crotalaria for forage. Fla. Agric. Exp. Stn. Bull. 361. 72 pp.Google Scholar
15. Rotar, P. P. and Joy, R. J. 1983. ‘Tropic Sun’ sunn hemp Crotalaria juncea L. Univ. of Hawaii Res. Ext. Ser. 036. 7 pp.Google Scholar
16. Smith, F. H. and Osborne, J. C. 1962. Toxic effects of Crotalaria seed. Vet. Med. 57:234–237.Google Scholar
17. Smith, L. W. and Culvenor, C.C.J. 1981. Plant sources of hepatotoxic pyrrolizidine alkaloids. J. Nat. Prod. 44:129–152.Google Scholar
18. Thomas, E. F. 1934. The toxicity of certain species of Crotalaria seed for the chicken, quail, turkey and dove. J. Am. Vet. Med. Assoc. 85:617–622.Google Scholar
19. Willette, R. E. and Cammarato, L. V. 1972. Phytochemical Survey of Connecticut I: Isolation of monocrotaline from Crotalaria sagittalis L. fruit. J. Pharm. Sci. 61:122.Google Scholar
20. Williams, M. C. 1982. 3-Nitropropionic acid and 3-nitro-1-propanol in species of Astragalus . Can. J. Bot. 60:1956–1963.Google Scholar