Hostname: page-component-8448b6f56d-cfpbc Total loading time: 0 Render date: 2024-04-16T15:48:32.068Z Has data issue: false hasContentIssue false
  • English
  • Français

Multiple-Herbicide Resistance in a 2,4-D–Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska

Published online by Cambridge University Press:  05 September 2017

Roberto J. Crespo ,
Ana B. Wingeyer ,
Greg R. Kruger ,
Chance W. Riggins ,
Patrick J. Tranel  and
Mark L. Bernards
Roberto J. Crespo*
Affiliation:
Graduate Research Assistant and Graduate Student, Department of Agronomy and Horticulture, University of Nebraska–Lincoln, Lincoln, NE 68583-0915
Ana B. Wingeyer
Affiliation:
Researcher, Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Paraná, and CONICET, Oro Verde, Entre Ríos, E3101, Argentina
Greg R. Kruger
Affiliation:
Associate Professor, West Central Research and Extension Center, University of Nebraska–Lincoln, North Platte, NE 69101-7751
Chance W. Riggins
Affiliation:
Postdoc Research Associate and Professor, Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Patrick J. Tranel
Affiliation:
Postdoc Research Associate and Professor, Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Mark L. Bernards
Affiliation:
Assistant Professor, Department of Agronomy and Horticulture, University of Nebraska–Lincoln, Lincoln, NE 68583-0915
*
*Corresponding author’s E-mail: rojacre@yahoo.com.ar
Get access Rights & Permissions [Opens in a new window]

Abstract

A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose–response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase–inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non–target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors.

Keywords

2,4-Dametrynaminocyclopyrachloraminopyralidatrazinechlorimuron-ethylglufosinateglyphosateimazethapyrlactofenmesotrionepicloramtall waterhemp, Amaranthus tuberculatus (Moq.) Sauer. AMATU.Cross-resistancedose–responseherbicide resistanceinjury
Type
Weed Biology and Ecology
Information
Weed Science , Volume 65 , Issue 6 , November 2017 , pp. 743 - 754
Copyright
© Weed Science Society of America, 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

a

Current address of first author: Crop Consultant, Oro Verde, Entre Ríos, E3101, Argentina

b

Current address of sixth author: Associate Professor, School of Agriculture, Western Illinois University, Macomb, IL 61455.

Associate Editor for this paper: Franck E. Dayan, Colorado State University

References

Literature Cited

Anderson, DD, Roeth, FW, Martin, AR (1996) Occurrence and control of triazine-resistant common waterhemp (Amaranthus rudis) in field corn (Zea mays). Weed Technol 10:570–575 Google Scholar
Beckie, HJ, Heap, IM, Smeda, RJ, Hall, LM (2000) Screening for herbicide resistance in weeds. Weed Technol 14:428–445 Google Scholar
Behrens, MR, Mutlu, N, Chakraborty, S, Dumitru, R, Jiang, WZ, LaVallee, VJ, Herman, PL, Clemente, TE, Weeks, DP (2007) Dicamba resistance: enlarging and preserving biotechnology-based weed management strategies. Science 316:1185–1188 Google Scholar
Bernards, ML, Crespo, RJ, Kruger, GR, Gaussoin, RE, Tranel, PJ (2012) A waterhemp (Amaranthus tuberculatus) population resistant to 2,4-D. Weed Sci 60:379–384 Google Scholar
Bernards, ML, Gaussoin, RE, Klein, RN, Knezevic, SZ, Kruger, GR, Lyon, DJ, Reicher, ZJ, Sandell, LD, Young, SL, Wilson, RG, Shea, PJ, Ogg, CL (2011) Guide for Weed Management in Nebraska. Lincoln, NE: University of Nebraska–Lincoln Extension EC130. 258 pGoogle Scholar
Burnside, OC (1996) The history of 2,4-D and its impact on development of the discipline of weed science in the United States. Pages 5–16 in Burnside, OC, ed. Biologic and Economic Assessment of Benefits from Use of Phenoxy Herbicides in the United States. Washington, DC: U.S. Department of Agriculture NAPIAP Report 1-PA-96 Google Scholar
Costea, M, Weaver, SE, Tardif, FJ (2005) The biology of invasive alien plants in Canada. Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea & Tardif. Can J Plant Sci 85:507–522 Google Scholar
Devine, MD, Preston, C (2000) The molecular basis of herbicide resistance. Pages 72–104 in Cobb AH, Kirkwood RC, eds. Herbicides and Their Mechanisms of Action. Sheffield, UK: Academic Google Scholar
Ellis, RH, Hong, TD, Roberts, EH (1985) Handbook of seed technology for Genebanks Vol. II. Compendium of specific germination information and text recommendations. Rome: International Board for Plant Genetic Resources, FAO. http://www.bioversityinternational.org/fileadmin/user_upload/online_library/publications/pdfs/52.pdf. Accessed July 29, 2017Google Scholar
Foes, MJ, Liu, L, Vigue, G, Stoller, EW, Wax, LM, Tranel, PJ (1999) A kochia (Kochia scoparia) biotype resistant to triazine and ALS-inhibiting herbicides. Weed Sci 47:20–27 Google Scholar
Foes, MJ, Tranel, PJ, Wax, LM, Stoller, EW (1998) Biotype of common waterhemp (Amaranthus rudis) resistant to triazine and ALS herbicides. Weed Sci 46:514–520 Google Scholar
Guo, J, Riggins, CW, Hausman, NE, Hager, AG, Riechers, DE, Davis, AS, Tranel, PJ (2015) Nontarget-site resistance to ALS inhibitors in waterhemp (Amaranthus tuberculatus). Weed Sci 65:399–407 Google Scholar
Gustafson, DI (2008) Sustainable use of glyphosate in North American cropping systems. Pest Manag Sci 64:409–416 Google Scholar
Hager, A, Sprague, C (2002) Weeds on the Horizon. Champaign, IL: University of Illinois Extension Bulletin Issue No. 6Google Scholar
Heap, I (2017) The International Survey of Herbicide Resistant Weeds. www.weedscience.com. Accessed: March 20, 2017Google Scholar
Hilton, HW (1957) Herbicide tolerant strain of weeds. Honolulu, HI: Hawaiian Sugar Planters Association Annual Reports. Pp 69–72Google Scholar
Hirschberg, J, McIntosh, L (1983) Molecular basis of herbicide resistance in Amaranthus hybridus . Science 222:1346–1349 Google Scholar
Horak, MJ, Peterson, DE (1995) Biotypes of Palmer amaranth (Amaranthus palmeri) and common waterhemp (Amaranthus rudis) are resistant to imazethapyr and thifensulfuron. Weed Technol 9:192–195 Google Scholar
Knezevic, SZ, Streibig, JC, Ritz, C (2007) Utilizing R software package for dose-response studies: the concept and data analysis. Weed Technol 21:840–848 Google Scholar
Lee, RM, Hager, AG, Tranel, PJ (2008) Prevalence of a novel resistance mechanism to PPO-inhibiting herbicides in waterhemp (Amaranthus tuberculatus). Weed Sci 56:371–375 Google Scholar
Lovell, ST, Wax, LM, Horak, MJ, Peterson, DE (1996) Imidazolinone and sulfonylurea resistance in a biotype of common waterhemp (Amaranthus rudis). Weed Sci 44:789–794 Google Scholar
McMullan, PM, Green, JM (2011) Identification of a tall waterhemp (Amaranthus tuberculatus) biotype resistant to HPPD-inhibiting herbicides, atrazine, and thifensulfuron in Iowa. Weed Technol 25:514–518 Google Scholar
Patzoldt, WL, Dixon, BS, Tranel, PJ (2003) Triazine resistance in Amaranthus tuberculatus (Moq.) Sauer that is not site-of-action mediated. Pest Manag Sci 59:1134–1142 Google Scholar
Patzoldt, WL, Hager, AG, McCormick, JS, Tranel, PJ (2006) A codon deletion confers resistance to herbicides inhibiting protoporphyrinogen oxidase. Proc Natl Acad Sci USA 103:12329–12334 Google Scholar
Patzoldt, WL, Tranel, PJ (2007) Multiple ALS mutations confer herbicide resistance in waterhemp (Amaranthus tuberculatus). Weed Sci 55:421–428 Google Scholar
Patzoldt, WL, Tranel, PJ, Hager, AG (2005) A waterhemp (Amaranthus tuberculatus) biotype with multiple resistance across three herbicide sites of action. Weed Sci 53:30–36 CrossRefGoogle Scholar
R Core Team (2014) R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing. http://www.R-project.org.Google Scholar
Schultz, JL, Chatham, LA, Riggins, CW, Tranel, PJ (2015) Distribution of herbicide resistances and molecular mechanisms conferring resistance in Missouri waterhemp (Amaranthus rudis Sauer) populations. Weed Sci 63:336–345 Google Scholar
Seefeldt, SS, Jensen, JE, Fuerst, EP (1995) Log-logistic analysis of herbicide dose–response relationships. Weed Technol 9:218–227 Google Scholar
Shoup, DE, Al-Khatib, K (2005) Fate of acifluorfen and lactofen in common waterhemp (Amaranthus rudis) resistant to protoporphyrinogen oxidase-inhibiting herbicides. Weed Sci 53:284–289 Google Scholar
Shoup, DE, Al-Khatib, K, Peterson, DE (2003) Common waterhemp (Amaranthus rudis) resistance to protoporphyrinogen oxidase inhibiting herbicides. Weed Sci 51:145–150 Google Scholar
Steckel, LE, Sprague, CL, Stoller, EW, Wax, LM, Simmons, FW (2007) Tillage, cropping system, and soil depth effects on common waterhemp (Amaranthus rudis) seed-bank persistence. Weed Sci 55:235–239 Google Scholar
Sterling, TM, Hall, JC (1997) Mechanism of action of natural auxins and the auxinic herbicides. Pages 111–141 in Roe RM, Burton JD, Kuhr RJ, eds. Herbicide Activity: Toxicology, Biochemistry and Molecular Biology. Amsterdam: IOS Press Google Scholar
Streibig, JC, Rudemo, M, Jensen, JE (1993) Dose-response curves and statistical models. Pages 29–55 in Herbicide Bioassays. Streibig JC, Kudsk P, eds. Boca Raton, FL: CRC Google Scholar
Switzer, CM (1957) The existence of 2,4-D resistant wild carrot. Pages 315–318 in Proceeding of the 11th Northeast Weed Control Conference. Riverhead, NY: Northeastern Weed Science SocietyGoogle Scholar
Tranel, PJ, Riggins, CW, Bell, MS, Hager, AG (2011) Herbicide resistances in Amaranthus tuberculatus: a call for new options. J Agric Food Chem 59:5808–5812 Google Scholar
Trucco, F, Tatum, T, Robertson, KR, Rayburn, AL, Tranel, PJ (2006) Characterization of waterhemp (Amaranthus tuberculatus) x smooth pigweed (A. hybridus) F1 hybrids. Weed Technol 20:14–22 Google Scholar
Webster, TM (2005) Weed survey—Southern states: broadleaf crops subsection. Pages 291–294 in Proceedings of the 58th Southern Weed Science Society Meeting. Honolulu, HI: Southern Weed Science SocietyGoogle Scholar
Wright, TR, Shan, G, Walsh, TA, Lira, JM, Cui, C, Song, P, Zhuang, M, Arnold, NL, Lin, G, Yau, K, Russell, SM, Cicchillo, RM, Peterson, MA, Simpson, DM, Zhou, N, Ponsamuel, J, Zhang, Z (2010) Robust crop resistance to broadleaf and grass herbicides provided by aryloxyalkanoate dioxygenase transgenes. Proc Natl Acad Sci USA 107:20240–20245 Google Scholar