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Evidence for the Interaction of an Imidazolinone Herbicide with Leucine, Valine, and Isoleucine Metabolism

Published online by Cambridge University Press:  12 June 2017

Paul C. Anderson  and
Kenneth A. Hibberd
Paul C. Anderson
Affiliation:
Molecular Genetics, Inc., 10320 Bren Rd. East, Minnetonka, MN 55343
Kenneth A. Hibberd
Affiliation:
Molecular Genetics, Inc., 10320 Bren Rd. East, Minnetonka, MN 55343
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Abstract

Effects of the imidazolinone herbicide AC243 997 [2-(5-isopropyl)-5-methyl-4-oxo-2-imidazolin-2-yl) nicotinic acid] on the growth of corn (Zea mays L. ‘Black Mexican Sweet’) cell suspension cultures were characterized. The herbicide caused half maximal growth inhibition at concentrations in the range of 10 nM to 30 nM. Significant reductions in protein synthesis were observed following treatment of cells with 10 μM herbicide. Analysis of free amino acid pool sizes indicated that levels of the biosyn-thetically related amino acids leucine and valine were substantially decreased in cells exposed to 10 μM herbicide. Supplementation of suspension culture growth media with leucine and valine plus isoleucine (1 mM each) reversed growth inhibitory effects of AC243 997 at levels ranging from 10 nM to 1 mM. The results suggest a specific interaction of this imidazolinone herbicide with leucine, valine, and isoleucine metabolism.

Keywords

Amino acidscell culturecornZea mays
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 33 , Issue 4 , July 1985 , pp. 479 - 483
Copyright
Copyright © 1985 by the Weed Science Society of America 

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