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Effects of Calcium, Magnesium, and Phosphate on Glyphosate Absorption by Cultured Plant Cells

Published online by Cambridge University Press:  12 June 2017

Todd L. Mervosh  and
Nelson E. Balke
Todd L. Mervosh
Affiliation:
Dep. Agron., Univ. Wisconsin, Madison, WI 53706
Nelson E. Balke
Affiliation:
Dep. Agron., Univ. Wisconsin, Madison, WI 53706
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Abstract

Effects of several inorganic ions on short-term glyphosate absorption by cultured plant cells were determined by measuring the glyphosate content of cells exposed to inorganic salts in a 0.01-mM glyphosate solution buffered at pH 5.7. Within 10 min, glyphosate absorption by potato and velvetleaf cells was stimulated about equally by 1.5 mM CaCl2 or 1.5 mM MgCl2 but was not affected by 1.5 mM KCl. Glyphosate absorption by both species increased linearly with log10 CaCl2 concentration; for velvetleaf cells, the response was greatest for CaCl2 concentrations greater than 0.2 mM. Over time of absorption, glyphosate concentration within velvetleaf cells approached an asymptote. Addition of 0.1 mM KH2PO4 inhibited glyphosate absorption by potato cells in the presence or absence of Ca2+ and Mg2+ salts; all other K+ salts tested had no effect. Glyphosate absorption by potato, velvetleaf, soybean, corn, and rice cells was stimulated by 5.0 mM CaCl2 but was inhibited by 0.1 mM KH2PO4 in the presence of 0.05 or 5.0 mM CaCl2.

Keywords

Glyphosate, N-(phosphonomethyl)glycinepotato, Solanum tuberosum L. ‘Red Pontiac’ and ‘Superior’velvetleaf, Abutilon theophrasti Medic. ♯ ABUTHsoybean, Glycine max (L.) Merr. ‘Corsoy’corn, Zea mays L. Black Mexican Sweet'rice, Oryza sativa L. ‘Calrose 76’Herbicide absorptioninorganic ionscationsanionssuspension cellsglyphosate/cation chelation
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 39 , Issue 3 , September 1991 , pp. 347 - 353
Copyright
Copyright © 1991 by the Weed Science Society of America 

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