Hostname: page-component-77c89778f8-gvh9x Total loading time: 0 Render date: 2024-07-20T08:40:23.533Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of Auxin-Like Herbicides on Nucleohistones in Cucumber and Wheat Roots

Published online by Cambridge University Press:  12 June 2017

L. G. Chen ,
A. Ali ,
R. A. Fletcher ,
C. M. Switzer  and
G. R. Stephenson
L. G. Chen
Affiliation:
Dep. of Environ. Biol., University of Guelph, Guelph, Ontario, Canada
A. Ali
Affiliation:
Dep. of Environ. Biol., University of Guelph, Guelph, Ontario, Canada
R. A. Fletcher
Affiliation:
Dep. of Environ. Biol., University of Guelph, Guelph, Ontario, Canada
C. M. Switzer
Affiliation:
Dep. of Environ. Biol., University of Guelph, Guelph, Ontario, Canada
G. R. Stephenson
Affiliation:
Dep. of Environ. Biol., University of Guelph, Guelph, Ontario, Canada
Get access Rights & Permissions [Opens in a new window]

Abstract

Roots of susceptible cucumber (Cucumis sativus L. ‘Chicago pickling’) and tolerant wheat (Triticum aestivum L. ‘Manitou’) seedlings were treated with 3,6-dichloro-o-anisic acid (dicamba) or (2,4-dichlorophenoxy)acetic acid (2,4-D) for either 10 or 46 hr. The roots were excised and nucleohistones were isolated and fractionated by polyacrylamide gel electrophoresis. In untreated cucumber roots there were four major nucleohistone fractions. Two of these fractions decreased or were not detectable 10 or 46 hr after treatment with dicamba or 2,4-D. In wheat, the nucleohistone fractions of the treated roots were similar to those of the controls. This suggests that in cucumber more of the DNA template is available for transcription. This suggestion was supported by the fact that the incorporation of 14C-adenine into RNA in cucumber roots 10 hr after treatment with dicamba was increased by 50%, whereas in wheat there was no difference. Furthermore, the incorporation of 14C-leucine into protein in cucumber roots 10 hr after treatment with dicamba was inhibited by 70%, indicating that the increased RNA produced was incapable of translating for protein synthesis. It is proposed that selective phytotoxicity of auxin-like herbicides is based on a differential alteration of RNA species and interference with protein synthesis.

Type
Research Article
Information
Weed Science , Volume 21 , Issue 3 , May 1973 , pp. 181 - 184
Copyright
Copyright © 1973 Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Arnold, W. E. and Nalewaja, J. D. 1971. Effect of dicamba on RNA and protein. Weed Sci. 19:301–305.CrossRefGoogle Scholar
2. Bonner, J., Chalkley, G. R., Dahmus, M., Fambrough, D., Fujimura, F., Huang, R. C., Huberman, J., Jensen, R., Marushige, K., Ohlenbusch, H., Olivera, B., and Widholm, J. 1968. Isolation and characterization of chromosomal nucleoproteins. Pages 3–65 in Colowich, P. and Kaplan, N. O., eds. in chief, Grossman, L. and Moldave, K., eds. this volume. Methods in enzymology, Vol. XII. Nucleic acid part B. Academic Press, New York and London.Google Scholar
3. Chen, L. G., Switzer, C. M. and Fletcher, R. A. 1972. Nucleic acid and protein changes induced by auxin-like herbicides. Weed Sci. 20:53–55.Google Scholar
4. Fletcher, R. A. 1969. Retardation of leaf senescence by benzyladenine in intact bean plants. Planta 89:1–8.CrossRefGoogle ScholarPubMed
5. Fites, R. C., Hanson, J. B., and Slife, F. W. 1969. Alteration of messenger RNA and ribosome synthesis in soybean hypocotyl by 2,4-D. Bot. Gaz. 130:118–126.Google Scholar
6. Huang, R. and Bonner, J. 1962. Histone, a suppressor of chromosomal RNA synthesis. Proc. Nat. Acad. Sci. U.S.A. 48:1216–1222.Google Scholar
7. Key, J. L. 1963. Studies on 2,4-D induced changes in ribonucleic acid metabolism in excised corn mesocotyl tissue. Weeds 11:177–181.CrossRefGoogle Scholar
8. Key, J. L. 1969. Hormones and nucleic acid metabolism. Annu. Rev. Plant Physiol. 20:449–474.Google Scholar
9. Key, J. L. and Shannon, J. C. 1964. Enhancement by auxin of ribonucleic acid synthesis in excised soybean hypocotyl tissue. Plant Physiol. 39:360–364.Google Scholar
10. Liao, S. H. and Hamilton, R. H. 1966. Intracellular localization of growth hormones in plants. Science 151:822–824.Google Scholar
11. O'Brien, T. J., Jarvis, B. C., Cherry, J. H., and Hanson, J. B. 1968. Enhancement by 2,4-D of chromatin RNA polymerase in soybean hypocotyl tissue. Biochem. Biophys. Acta. 169:35–43.Google Scholar
12. Panyim, S. and Chalkley, R. 1969. High resolution acrylamide gel electrophoresis of histones. Arch. Biochem. Biophys. 130:337–346.Google Scholar
13. Tester, C. F. and Dure, L. S. 1967. Nucleic acid synthesis during the hormone stimulated growth of excised oat coleoptiles. Biochemistry 6:2532–2537.Google Scholar