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Effects of Ammonium Thiocyanate on Carbohydrate Metabolism in the Cotton Plant

Published online by Cambridge University Press:  12 June 2017

Yih-Fen Wu  and
Eddie Basler
Yih-Fen Wu
Affiliation:
Oklahoma Agricultural Experiment Station, Stillwater, Oklahoma
Eddie Basler
Affiliation:
Oklahoma Agricultural Experiment Station, Stillwater, Oklahoma
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Abstract

Some effects of ammonium thiocyanate (hereinafter referred to as NH4SCN) on the starch and reducing sugar content, amylase activity, photosynthetic O2 release, Hill reaction, and glycine-14C incorporation into amino acids, organic acids, and sugar fractions in excised leaf and cotyledonary tissue of cotton (Gossypium hirsutum L., var. Acala 44) were determined. The starch content of leaf tissue floated on Hoaglands nutrient solution containing 2000 ppm NH4SCN decreased at 1 and 2 days treatment. The amylase activity of excised cotyledons was increased by 2000 and 5000 ppm NH4SCN and the reducing sugar of the same tissue was decreased. Photosynthesis of small leaf disks, as measured by O2 exchange, was decreased and respiration was increased by 2000 ppm NH4SCN. Photosynthesis in isolated chloroplasts, as measured by the reduction of 2,6-dichlorophenolin dophenol in the Hill reaction, also was inhibited by NH4SCN at concentrations above 2000 ppm. Ammonium thiocyanate at 2000 ppm inhibited the conversion of glycine to sugars but had no effect on conversion of glycine to organic acids in leaf tissue.

Type
Research Article
Information
Weed Science , Volume 17 , Issue 3 , July 1969 , pp. 362 - 365
Copyright
Copyright © 1969 Weed Science Society of America 

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