Hostname: page-component-84b7d79bbc-c654p Total loading time: 0 Render date: 2024-07-25T10:34:20.786Z Has data issue: false hasContentIssue false
  • English
  • Français

Direct Liquid Scintillation Radioassay of 14C-Labeled Herbicides in Soil

Published online by Cambridge University Press:  12 June 2017

T. L. Lavy ,
C. G. Messersmith  and
H. W. Knoche
T. L. Lavy
Affiliation:
Dep. of Agron., Univ. of Nebraska, Lincoln 68503
C. G. Messersmith
Affiliation:
Dep. of Agron., North Dakota State Univ., Fargo 58103
H. W. Knoche
Affiliation:
Dep. of Biochem., Univ. of Nebraska, Lincoln 68503
Get access Rights & Permissions [Opens in a new window]

Abstract

A liquid scintillation counting solution was developed which optimized the direct assay efficiencies of 14C-labeled 2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine (atrazine), 3,6-dichloro-o-anisic acid (dicamba), and α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine (trifluralin) in soil. In this counting technique, 3-g soil samples containing 14C-labeled herbicide were placed directly into a liquid scintillation vial containing a phosphorescent counting solution. Samples were shaken and allowed to settle before assaying using a liquid scintillation spectrometer. This direct radioassay was as efficient or more efficient than double or single extraction processes. Automatic external standardization in conjunction with the liquid scintillation counter was an effective tool for estimating the 14C present in soil samples.

Type
Research Article
Information
Weed Science , Volume 20 , Issue 3 , May 1972 , pp. 215 - 219
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Deming, J. M. 1963. Determination of volatility losses of C14-CDAA from soil surfaces. Weeds 11:19–96.Google Scholar
2. Farmer, W. J. and Jensen, C. R. 1970. Diffusion and analysis of carbon-14 labeled dieldrin in soils. Soil Sci. Soc. Amer. Proc. 34:28–31.Google Scholar
3. Funt, B. L. 1956. Scintillating gels. Nucleonics 14(8):83–84.Google Scholar
4. Kearney, P. C., Sheets, T. J., and Smith, J. W. 1964. Volatility of seven s-triazines. Weeds 12:83–87.CrossRefGoogle Scholar
5. Lavy, T. L. 1968. Micromovement mechanisms of s-triazines in soil. Soil Sci. Soc. Amer. Proc. 32:377–380.Google Scholar
6. Messersmith, C. G., Burnside, O. C., and Lavy, T. L. 1971. Biological and non-biological dissipation of trifluralin from soil. Weed Sci. 19:285–290.Google Scholar
7. Page, A. L., Sims, J. R., and Bingham, F. T. 1964. The use of liquid scintillation for the assay of carbon-14 in clay and soil suspensions. Soil Sci. Soc. Amer. Proc. 28:359–363.Google Scholar
8. Weed Science Society of America. 1970. Herbicide Handbook of the Weed Science Society of America. 2nd Ed. W. F. Humphrey Press, Inc. Geneva, N. Y. 368 p.Google Scholar