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Determination of Arsenical Herbicide Residues in Plant Tissues

Published online by Cambridge University Press:  12 June 2017

R. M. Sachs ,
J. L. Michael ,
F. B. Anastasia  and
W. A. Wells
F. B. Anastasia
Affiliation:
Plant Sci. Lab., Fort Detrick, Frederick, Maryland
W. A. Wells
Affiliation:
Plant Sci. Lab., Fort Detrick, Frederick, Maryland
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Abstract

Paper chromatographic separation of hydroxydimethylarsine oxide (cacodylic acid), monosodium methanearsonate (MSMA), sodium arsenate, and sodium arsenite was achieved with the aid of four solvent systems. Aqueous extracts of plant tissues removed essentially all the arsenicals applied, but methanolic fractionation was required before the extracts could be analyzed by paper chromatographic procedures. A standard nitric-sulfuric acid digestion procedure was employed for arsenic analyses, but great care was taken to avoid sulfuric-acid-induced charring by first adding relatively large amounts of nitric acid to drive off chlorides present. Depending upon the amount of chloride present, substantial losses of arsenic as arsine chlorides were observed if the samples charred. Five minutes in fuming sulfuric acid to completely break the carbon-arsenic bonds was another critical requirement for the quantitative determination of arsenic from cacodylic acid and MSMA. The silver diethyldithiocarbamate colorimetric method was useful for detecting as little as 0.6 μg or as much as 20 μg of arsenic per sample.

Type
Research Article
Information
Weed Science , Volume 19 , Issue 4 , July 1971 , pp. 412 - 416
Copyright
Copyright © Weed Science Society of America 

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References

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