Hostname: page-component-77c89778f8-5wvtr Total loading time: 0 Render date: 2024-07-18T16:55:40.188Z Has data issue: false hasContentIssue false

A Microradioautographic Study of 14C-Diuron Absorption by Cotton

Published online by Cambridge University Press:  12 June 2017

R. H. Strang
Affiliation:
Dep. of Plant Path., Louisiana State Univ., Baton Rouge, Louisiana 70803
R. L. Rogers
Affiliation:
Dep. of Plant Path., Louisiana State Univ., Baton Rouge, Louisiana 70803

Abstract

The techniques of cryostat sectioning, freeze-drying, and microradioautography were adapted to study the absorption and translocation of radio-labeled 3-(3,4-dichlorophenyl)-1,1-dimethylurea (14C-diuron) in root-treated cotton (Gossypium hirsutum L., var. Coker 201) plants. Diuron appeared to move primarily in the apoplast and to be transported acropetally and laterally in the transpiration stream. Radioactivity was observed to accumulate in striking concentrations in the lysigenous, or pigment, glands and the trichomes of the cotton plant. This accumulation is postulated to be a major factor in lowering the effective concentration of the herbicide in the leaves of cotton as compared to leaves of more susceptible plants, and thus may be a significant factor in the tolerance of cotton to diuron.

Type
Research Article
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Anonymous. 1964. Nuclear physics and autoradiography. Kodak Pamphlet No. P-64.Google Scholar
2. Bayer, D. E. and Yamaguchi, S. 1965. Absorption and distribution of diuron-C14 . Weeds 13:232235.CrossRefGoogle Scholar
3. Beradri, L. C. and Frampton, V. L. 1957. Note on gossypol and its relation to color fixation in cotton seed oil. J. Amer. Oil Chem. Soc. 34:399401.Google Scholar
4. Biddulph, S., Biddulph, O., and Cory, R. 1958. Visual indications of upward movement of foliar applied P32 and C14 in the phloem of the bean stem. Amer. J. Bot. 45:648.CrossRefGoogle Scholar
5. Bishop, N. I. 1958. The influence of the herbicide DCMU on the oxygen evolving system of photosynthesis. Biochem. Biophys. Acta. 27:205206.CrossRefGoogle ScholarPubMed
6. Boatner, C. J., Hall, C. M., Rollins, M. L., and Castillon, L. E. 1947. Pigment glands of cotton seed. II. Nature and properties of gland walls. Bot. Gaz. 108:484491.CrossRefGoogle Scholar
7. Boatner, C. J., Hall, C. M., O'Connor, R. T., and Castillon, L. E. 1947. Pigment glands of cottonseed. III. Distribution and some properties of cottonseed pigments. Bot. Gaz. 109:108120.CrossRefGoogle Scholar
8. Branton, D. and Jacobson, L. 1961. Freeze-drying of plant material. Exp. Cell Res. 22:559568.CrossRefGoogle Scholar
9. Bucha, H. C. and Todd, C. W. 1951. 3-(p-chlorophenyl)-1,1-dimethylurea–A new herbicide. Science 114:493494.CrossRefGoogle ScholarPubMed
10. Callaghan, J. J. and Grun, P. 1961. Incorporation of C14-labeled maleic hydrazide into the root-tip cells of Allium cernum, Vicia faba and Tradescantia paludosa . J. Biophys. Biochem. Cytol. 10:567575.CrossRefGoogle Scholar
11. Christopher, R. J. and Fisk, E. L. 1954. Responses of plants to the herbicide 3-(p-chlorophenyl)-1,1-dimethylurea (CMU). Bot. Gaz. 116:114.CrossRefGoogle Scholar
12. Cooke, A. R. 1956. A possible mechanism of action of the urea type herbicides. Weeds 4:397398.CrossRefGoogle Scholar
13. Cotner, R. C., Hamilton, R. J., Mumma, R. O., and Frear, D. E. H. 1968. Localization of dieldrin in wheat tissue. J. Agr. Food Chem. 16:608609.CrossRefGoogle Scholar
14. Crafts, A. S. 1959. Further studies on comparative mobility of labeled herbicides. Plant Physiol. 34:613620.CrossRefGoogle ScholarPubMed
15. Crafts, A. S. and Yamaguchi, S. 1958. Comparative tests on the uptake by Zebrina pendula and Tradescantia flaminensis . Hilgardia 27:421454.CrossRefGoogle Scholar
16. Crafts, A. S. and Yamaguchi, S. 1960. Absoption of herbicides by roots. Amer. J. Bot. 47:248255.CrossRefGoogle Scholar
17. Dechary, J. M. 1957. The pigments of crude cottonseed oils. J. Amer. Oil. Chem. Soc. 34:597600.CrossRefGoogle Scholar
18. Dechary, J. M. and Brown, L. E. 1956. Substituted arylimino derivatives of gossypol. J. Amer. Oil. Chem. Soc. 33:7678.CrossRefGoogle Scholar
19. Duffer, W. M. Jr. 1957. Autoradiography with plant tissue. Bot. Rev. 23:351387.Google Scholar
20. Earle, T. T. 1951. Tracer studies with alligator weed using 2,4-D-C14 . Science 114:695696.CrossRefGoogle ScholarPubMed
21. Fried, M. and Franklin, A. H. 1961. Rapid preparation of fresh frozen sections of barley roots. Nature 189:414415.CrossRefGoogle Scholar
22. Gage, R. S. and Arnoff, S. 1960. Radioautography of tritiated photosynthate arising from HTO. Plant Physiol. 35:65.CrossRefGoogle ScholarPubMed
23. Geissbühler, H. C., Hasselback, C., Aebi, H., and Ebner, L. 1963. The fate of N′-(4-chlorophenoxy)-phenyl-N,N-dimethylurea in soils and plants. II. Uptake and distribution within plants. Weed Res. 3:181194.CrossRefGoogle Scholar
24. Good, N. E. 1961. Inhibitors of the Hill reaction. Plant Physiol. 36:788803.CrossRefGoogle ScholarPubMed
25. Haun, J. R. and Peterson, J. H. 1954. Translocation of 3-(p-chlorophenyl)-1,1-dimethylurea in plants. Weeds 3:177187.CrossRefGoogle Scholar
26. Jagendorf, A. T. 1958. The relationship between electron transport and phosphorylation in spinach chloroplasts. Brookhaven Symposia in Biol. 11:236257.Google Scholar
27. Jensen, W. A. 1962. Botanical Histochemistry. W. H. Freeman and Co., San Francisco. 408 p.Google Scholar
28. Jensen, W. A. and Kavaljian, L. G. 1956. The cytochemical localization of ascorbic acid in root tip cells. J. Biophys. and Biochem. Cytol. 2:8792.CrossRefGoogle ScholarPubMed
29. McIlrath, W. J. 1950. The adaptability of several histological techniques to the preparation of P-32 radioautographs from plant tissues. Proc. Iowa Acad. Sci. 57:135140.Google Scholar
30. McMichael, S. C. 1959. Hopi cotton, a source of cottonseed free of gossypol pigments. Agron. J. 51:630.CrossRefGoogle Scholar
31. Metcalfe, C. R. and Chalk, L. 1950. Anatomy of the dicotyledons. Clarendon Press, Oxford. 1500 p.Google Scholar
32. Minshall, W. H. 1954. Translocation path and place of action of 3-(4-chlorophenyl)-1,1-dimethylurea in bean and tomato. Can. J. Bot. 32:795798.CrossRefGoogle Scholar
33. Moreland, D. E. and Hill, K. L. 1962. Interference of herbicides with the Hill reaction of isolated chloroplasts. Weeds 10:229236.CrossRefGoogle Scholar
34. Muzik, T. J., Cruzado, H. J., and Loustalot, A. J. 1954. Studies on the absorption, translocation, and action of CMU. Bot. Gaz. 116:6573.CrossRefGoogle Scholar
35. Perkins, J. H., Nelson, C. D., and Gorham, P. R. 1959. A tissue-autoradiographic study of the translocation of C14-labeled sugars in the stems of young soybean plants. Can. J. Bot. 37:871877.CrossRefGoogle Scholar
36. Radwan, M. A., Stocking, C. R., and Currier, H. B. 1960. Histoautoradiographic studies of herbicidal translocation. Weeds 8:657665.CrossRefGoogle Scholar
37. Rogers, R. L. and Funderburk, H. H. Jr. 1968. Physiological aspects of fluometuron. J. Agr. Food Chem. 16:434440.CrossRefGoogle Scholar
38. Smith, J. W. and Sheets, T. J. 1966. Uptake, distribution and metabolism of monuron and diuron by several plants. J. Agr. Food Chem. 15:577581.CrossRefGoogle Scholar
39. Stocking, C. R. 1956. Excretion by glandular organs. In W. Ruhland ed. Handbuch der Pflanzenphysiologie 3:503510. Springer-Verlag, Berlin, 1073 p.Google Scholar
40. Wessels, J. S. C. and van derVeen, R. 1956. The action of some derivatives of phenylurethan and of 3-(p-chlorophenyl)-1,1-dimethylurea on the Hill reaction. Biochem. Biophys. Acta. 19:548549.CrossRefGoogle Scholar