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Targeted delivery of propionate to the colon stimulates the release of anorectic gut hormones and suppresses appetite in humans

Published online by Cambridge University Press:  20 May 2014

E. S. Chambers
Affiliation:
Nutrition and Dietetic Research Group, University of Glasgow, G75 0QF, UK
A. Viardot
Affiliation:
Nutrition and Dietetic Research Group, University of Glasgow, G75 0QF, UK
A. Psichas
Affiliation:
Nutrition and Dietetic Research Group, University of Glasgow, G75 0QF, UK
D. J. Morrison
Affiliation:
Scottish Universities Environmental Research Centre, University of Glasgow, G75 0QF, UK
K. G. Murphy
Affiliation:
Section of Investigative Medicine, Imperial College London, W12 0NN, UK
S. E. K. Zac-Varghese
Affiliation:
Section of Investigative Medicine, Imperial College London, W12 0NN, UK
K. MacDougall
Affiliation:
School of Science, University of the West of Scotland, Paisley PA1 2BE, UK
T. Preston
Affiliation:
Scottish Universities Environmental Research Centre, University of Glasgow, G75 0QF, UK
M. C. Tedford
Affiliation:
School of Science, University of the West of Scotland, Paisley PA1 2BE, UK
G. Finlayson
Affiliation:
Institute of Psychological Sciences, University of Leeds, LS2 9JT, UK
J. E. Blundell
Affiliation:
Institute of Psychological Sciences, University of Leeds, LS2 9JT, UK
W. S. Dhillo
Affiliation:
Section of Investigative Medicine, Imperial College London, W12 0NN, UK
S. R. Bloom
Affiliation:
Section of Investigative Medicine, Imperial College London, W12 0NN, UK
W. G. Morley
Affiliation:
Leatherhead Food Research, Leatherhead KT22 7RY, UK
S. Clegg
Affiliation:
Leatherhead Food Research, Leatherhead KT22 7RY, UK
G. Frost
Affiliation:
Nutrition and Dietetic Research Group, University of Glasgow, G75 0QF, UK
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Abstract

Type
Abstract
Copyright
Copyright © The Authors 2014 

Recent evidence suggests that short chain fatty acids (SCFA) trigger the release of the anorectic gut hormones peptide YY (PYY) and glucagon like peptide-1 (GLP-1) by stimulating the free fatty acid receptors (FFAR) 2 and 3 on enteroendocrine L cells( Reference Tolhurst, Heffron and Lam 1 ). Of the SCFA produced by colonic fermentation of available carbohydrates, propionate has been shown to have the highest affinity for FFAR2( Reference Brown, Goldsworthy and Barnes 2 ). We hypothesised that increasing colonic propionate would stimulate gut hormone secretion and reduce energy intake. To elevate colonic propionate levels we have developed a novel propionate ester molecule whereby propionate is chemically bound by an ester bond to inulin. The majority of the bound propionate should only be released when the inulin polymer is fermented by the colonic microbiota, thus providing targeted colonic delivery.

To assess the site and extent of propionate release, 9 volunteers consumed a standardised breakfast containing 100 mg of 13C labelled propionate ester and 10  g of unlabelled ester. Breath H2 and 13CO2 enrichment were collected over 24 h to investigate gut transit times. Breath H2 started to increase at 180 min and peaked at 240 min. More than 80% of the 13C recovered in breath appeared co-incident with and after breath H2 onset, suggesting delivery of the majority of the bound propionate to the colon.

Plasma PYY levels after ingestion of propionate ester vs. control. Dotted lines signify the time point after which >80% propionate ester enters the colon. Data are presented as means ± sem, *P < 0.05.

This propionate ester (10 g/d) and an inulin control (10 g/d) were then administered to 20 volunteers in a randomised crossover study to determine its effect on food intake and gut hormone concentrations. Ingestion of 10 g propionate ester significantly increased plasma PYY and GLP-1 (P < 0.05) and reduced ad libitum food intake (1175 ± 103 kcal control vs. 1013 ± 94 kcal propionate ester; p < 0.01).

In conclusion, these data suggest that an acute increase in colonic propionate can elevate plasma PYY and GLP-1 levels and reduce food intake in humans. Elevating propionate levels in the colon may therefore offer a potential strategy to protect against weight gain and the metabolic consequences of obesity.

This work was supported by the Biotechnology & Biological Sciences Research Council (BB/H004971/1). All studies were conducted in accordance with the Declaration of Helsinki.

References

1. Tolhurst, G, Heffron, H, Lam, YS, et al. (2011) Diabetes 61, 364371.CrossRefGoogle Scholar
2. Brown, AJ, Goldsworthy, SM, Barnes, AA, et al. (2003) J Biol Chem 278, 1131211319.CrossRefGoogle Scholar
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