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Arterio–venous differences to study macronutrient metabolism: introduction and overview

Published online by Cambridge University Press:  12 June 2007

I. A. Macdonald
I. A. Macdonald*
Affiliation:
School of Biomedical Sciences, University of Nottingham Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK
*
Corresponding Author: Professor I. A. Macdonald, fax +44 (0)115 9709259, email Ian.Macdonald@nottingham.ac.uk
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Abstract

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The arterio-venous difference technique is now well established in the study of organ and tissue metabolism. This technique requires samples to be obtained of the arterial blood supplying and the venous drainage from a tissue, together with a measurement of the blood flow through the tissue. The technique is most appropriate when the arterial concentration and tissue metabolism of a substance are constant, and when the blood flow is stable. If these criteria are not satisfied, care is needed in the interpretation of the results obtained. It should be recognized that the arterio-venous difference technique only measures the net exchange of a substance with the tissue, and that tracers are needed if unidirectional flux needs to be estimated. The other factors which must be borne in mind when intending to use this technique are the transit times of blood and the substance of interest through a tissue, the volume of distribution of the substance in the tissue, and the possibility that the venous samples obtained are derived from a mixture of different tissues.

Keywords

Arterio-venous differenceTissue metabolismFick PrincipleArterialization
Type
Meeting Report
Information
Proceedings of the Nutrition Society , Volume 58 , Issue 4 , November 1999 , pp. 871 - 875
Copyright
The Nutrition Society

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