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Identification of Hydrangeaceae accessions of wild origin from Jeju, Korea, using molecular markers

Published online by Cambridge University Press:  24 September 2010

Young Hee Joung ,
Jeung Keun Suh ,
Nam Sook Lee ,
Sang Mi Eum ,
Ik-Young Choi  and
Mark S. Roh
Young Hee Joung
Affiliation:
Chonnam National University, School of Biological Science and Technology, Gwangju, Jeonnam, Korea
Jeung Keun Suh
Affiliation:
Dankook University, School of Bio-Resources Science, Laboratory of Floriculture and Plant Physiology, Cheonan, Chungnam, Korea
Nam Sook Lee
Affiliation:
Ewha Womans University, Department of Life Science, Seoul120-750, Korea
Sang Mi Eum
Affiliation:
Ewha Womans University, Department of Life Science, Seoul120-750, Korea
Ik-Young Choi
Affiliation:
National Instrumentation Center for Environmental Management, Seoul National University, Seoul, Korea
Mark S. Roh*
Affiliation:
US Department of Agriculture, Agricultural Research Service, US National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, MD20705, USA
*
*Corresponding author. E-mail: mark.roh@ars.usda.gov
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Abstract

Hydrangea and Schizophragma are popular horticultural and ornamental plants. This study was designed to identify unknown accessions of Hydrangeaceae that have leaf morphological characters different from Hydrangeaanomala subsp. petiolaris and Schizophragma hydrangeoides in Jeju, Korea. A total of 29 accessions of Hydrangea and Schizophragma from the wild were collected and analyzed using randomly amplified polymorphic DNA (RAPD) and single nucleotide polymorphisms (SNPs) from the ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene. Morphological characteristics of leaves and shoot apices of several accessions were also examined. Based on the RAPD markers, SNPs of the (rbcL) gene and morphological characteristics of apical buds, the unknown accessions were identified as H. anomala var. petiolaris.

Keywords

germplasmHydrangeaanomala subsp. petiolarismorphologynucleotide polymorphisms (SNPs)Schizophragma hydrangeoides
Type
Research Article
Information
Plant Genetic Resources , Volume 8 , Issue 3 , December 2010 , pp. 235 - 241
Copyright
Copyright © NIAB 2010

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References

Anderson, E (1933) Bulletin of Popular Information. Vol. I Series 4, No. 10. Boston, MA: Arnold Arboretum, Harvard University, pp. 53–56.Google Scholar
Brooks, AJ (1999) The essence of SNPS. Gene 234: 177–186.CrossRefGoogle Scholar
Dirr, MA (1990) Manual of Woody Landscape Plans: Their Identification, Ornamental Characteristics, Culture, Propagation and Uses. Champaign, IL: Stipes Publ. Co.Google Scholar
Hufford, L, Moody, ML and Soltis, DE (2001) A phylogenetic analysis of Hydrangeaceae based on sequences of the plastid gene matK and their combination with rbcL and morphological data. International Journal of Plant Science 162: 835–846.CrossRefGoogle Scholar
Jones, CJ, Edwards, KJ, Castaglikone, S, Winfield, MO, Sala, F, van de Wiel, C, Bredemeijer, G, Vosman, B, Matthes, M, Daly, A, Brettschneider, R, Bettini, P, Buiatti, M, Maestri, E, Malcevschi, A, Marmiroli, N, Aert, R, Volckaert, G, Rueda, J, Linacero, R, Vazquez, A and Karp, A (1997) Reproducibility testing of RAPD, AFLP, and SSR markers in plants by a network of European laboratories. Molecular Breeding 3: 381–390.CrossRefGoogle Scholar
Joung, YH and Roh, MS (2004) Paternity determination of Ornithogalum seedlings using DNA markers. Journal of Horticultural Science and Biotechnology 79: 316–321.CrossRefGoogle Scholar
Joung, YH and Roh, MS (2006) Mapping characterization of Pinus sylvestris var. sylvestriformis based on chloroplast DNA microsatellite markers. Forest Genetics 12: 89–98.Google Scholar
Kumar, S, Tamura, K and Nei, M (2004) Mega 3: integrated software for molecular evolutionary genetics analysis and sequence alignments. Briefings in Bioinformatics 5: 150–163.CrossRefGoogle Scholar
Lee, NS, Yeau, SH, Park, JO and Roh, MS (2006) Molecular evident for hybridization of Ilex × wandoensis (Aquifoliaceae) by RAPD analysis. Journal of Plant Biology 49: 491–497.CrossRefGoogle Scholar
Marth, GT, Korf, I, Yandell, MD, Yeh, RT, Gu, Z, Zakeri, H, Stitziel, NO, Hillier, L, Kwok, PY and Gish, WR (1999) A general approach to single-nucleotide polymorphism discovery. Nature Genetics 23: 452–456.CrossRefGoogle ScholarPubMed
Mortreau, E, Bertrand, H, Lambert, G and Laemand, J (2003) Collection of Hydrangea: genetic resources characterization. Acta Horticulturae 623: 231–238.CrossRefGoogle Scholar
Penna, G, Bush, A, Wise, R, Kim, W, Domier, L, Kasha, K, Laroche, Al, Scoles, G, Molnar, SJ and Fedak, G (1993) Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories. PCR Methods and Applications 2: 341–345.Google Scholar
Reed, SM and Rinehart, TA (2007) SSR marker analysis of genetic relationships within Hydrangea macrophylla. Journal of the American Society for Horticultural Science 132: 341–351.CrossRefGoogle Scholar
Rehder, A (1940) Manual of Cultivated Trees and Shrubs. Caldwell, NJ: Blackburn Press, p. 996.Google Scholar
Rinehart, TA, Scheffler, BE and Reed, SM (2006) Genetic diversity estimates for the genus Hydrangea and development of a molecular key based on SSR. Journal of the American Society for Horticultural Science 131: 787–797.CrossRefGoogle Scholar
Roh, MS, Lee, A-K, Suh, J-K and Slovin, J (2006) Confirmation of cross-pollination of Ardisia crenata by sequence-characterized amplified region (SCAR) markers. Scientia Horticulturae 109: 361–367.CrossRefGoogle Scholar
Soltis, DE, Xiang, Q-Y and Hufford, L (1995) Relationships and evolution of Hydrangeaceae based on rbcL sequence data. Amerian Journal of Botany 82: 504–514.CrossRefGoogle Scholar
Williams, JGK, Kubelik, AR, Livak, KJ, Rafalski, JA and Tingey, SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531–6535.CrossRefGoogle ScholarPubMed
Wolfe, AD, Xiang, Q-Y and Hephart, SR (1998) Assessing hybridization in natural populations of Penstemon (Scrophulariaceae) using hypervariable intersimple sequence repeat (ISSR) bands. Molecular Ecology 7: 1107–1125.CrossRefGoogle ScholarPubMed
Xie, GW (1997) Phytogeographical affinities of forest floras between China and Japan. Journal of Forest Research 8: 87–90.Google Scholar