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Ultrastructural immunocytochemical localization of two hydatid fluid antigens (antigen 5 and antigen B) in the brood capsules and protoscoleces of ovine and equine Echinococcus granulosus and E. multilocularis

Published online by Cambridge University Press:  06 April 2009

Caroline Davies ,
M. D. Rickard ,
D. T. Bout  and
J. P. Smyth
Caroline Davies
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London SW7
M. D. Rickard
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London SW7
D. T. Bout
Affiliation:
Services d'Immunologie et de Biologie Parasitaire de la Faculté de Médecine et de l'Institut Pasteur, Lille
J. P. Smyth
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London SW7
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Summary

The unlabelled antibody method was used in the ultrastructural localization of two hydatid fluid antigens, antigen 5 and antigen B, in brood capsules and protoscoleces of Echinococcus granulosus and E. multilocularis. Antigen 5 was found in the parenchyma cells of the protoscolex and brood capsule wall and to a lesser extent in the walls of the flame cells and collecting ducts of the excretory system and in the surrounding interstitial material. It is suggested that, while some excretion of this antigen may occur from the protoscolex, it could also be liberated into the cystic cavity by degeneration of protoscoleces and parenchymal cells of the brood capsule wall. Antigen B was found mainly in the distal cytoplasm and perinuclear cytoplasm of the tegument anterior to the suckers. It is apparently secreted to the outside and was present in the brood capsule contents; it adheres to the anterior surface and the posterior periodic acid–Schiff (PAS)-positive glycocalyx of the protoscolex and to the inner surface of the brood capsule wall. The protoscolex tegument posterior to the suckers was negative. The parenchyma cells of the protoscolex and brood capsule wall were also positive although the intensity of the reaction product was variable.

Type
Research Article
Information
Parasitology , Volume 77 , Issue 2 , October 1978 , pp. 143 - 152
Copyright
Copyright © Cambridge University Press 1978

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