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Trypanosoma congolense: interactions between trypanosomes expressing different metacyclic variable antigen types in vitro and in vivo

Published online by Cambridge University Press:  06 April 2009

C. J. Prain  and
C. A. Ross
C. J. Prain
Affiliation:
Centre for Tropical Veterinary Medicine, University of Edinburgh, Easter Bush, Roslin, Midlothian, Scotland
C. A. Ross*
Affiliation:
Centre for Tropical Veterinary Medicine, University of Edinburgh, Easter Bush, Roslin, Midlothian, Scotland
*
†To whom reprint requests should be sent.
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Summary

In Trypanosome congolense, the surface antigens expressed by cultured metacyclic forms are a limited and serodeme-specific subset of variable antigen types (VATs). Experiments were carried out in mammalian-form (MF) cultures, comprising dividing trypanosomes which express metacyclic VATs (M-VATs) maintained in vitro with a mammalian cell feeder layer, or in mice following infection with cultured metacyclic populations. Selective neutralization experiments were performed by incubating populations of trypanosomes with one or a combination of McAbs to remove their respective M-VATs. Deletion of one M-VAT from the population had no effect on expression and growth of other unrelated M-VATs. Deleted M-VATs were detected in very small proportions of the total population 2–7 days following neutralization. Subsequent incubation of neutralized cultures resulted in growth of deleted M-VATs which, in most cases, reached pre-neutralization levels. It appears, therefore, that in conditions where expression of M-VATs on dividing trypanosomes occurs, removal of individual M-VATs results in their re-expression within the population.

Type
Research Article
Information
Parasitology , Volume 97 , Issue 2 , October 1988 , pp. 277 - 286
Copyright
Copyright © Cambridge University Press 1988

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Footnotes

*

New England Biolabs Inc., 32 Tozer Road, Beverly, Ma, USA 01915–9990.

References

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