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Transmission electron microscopy on a case of Cyclospora cayetanensis infection from an immune-competent case confirms and extends prior detailed descriptions of its notably small endogenous stage

Published online by Cambridge University Press:  08 June 2022

Jitender P. Dubey Open the ORCID record for Jitender P. Dubey [Opens in a new window] ,
Jon E. Charlesworth  and
Bobbi S. Pritt
Jitender P. Dubey*
Affiliation:
Animal Parasitic Disease Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Building 1001, BARC-East, Beltsville, MD 20705-2350, USA
Jon E. Charlesworth
Affiliation:
Microscopy and Cell Analysis Core, Mayo Clinic, Rochester, MN 55905, USA
Bobbi S. Pritt
Affiliation:
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
*
Author for correspondence: Jitender P. Dubey, E-mail: jitender.dubey@usda.gov
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Abstract

Although infections with Cyclospora cayetanensis are prevalent worldwide, many aspects of this parasite's life cycle remain unknown. Humans are the only known hosts, existing information on its endogenous development has been derived from histological examination of only a few biopsy specimens. In histological sections, its stages are less than 10 μm, making definitive identification difficult. Here, confirmation of cyclosporiasis in a duodenal biopsy specimen from an 80-year-old man without any recognized immunodeficiency patient is reported. Asexual forms (schizonts) and sexual forms (gamonts) were located within enterocytes, including immature and mature schizonts, an immature male gamont and a female gamont. Merozoites were small (<5 μm × 1 μm) and contained two rhoptries, subterminal nucleus and numerous micronemes and amylopectin granules. These parasite stages were like those recently reported in the gallbladder of an immunocompromised patient, suggesting that the general life-cycle stages are not altered by immunosuppression.

Keywords

Cyclospora cayetanensiselectron microscopyhumanslife cycle
Type
Research Article
Information
Parasitology , Volume 149 , Issue 11 , September 2022 , pp. 1397 - 1405
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Creative Commons
To the extent this is a work of the US Government, it is not subject to copyright protection within the United States. Published by Cambridge University Press.
Copyright
Copyright © United States Department of Agriculture and the Author(s), 2022

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