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Studies on the amphid specific glycoprotein gp32 in different life-cycle stages of Meloidogyne species

Published online by Cambridge University Press:  06 April 2009

G. R. Stewart ,
R. N. Perry  and
D. J. Wright
G. R. Stewart
Affiliation:
1Department of Biology, Imperial College, Silwood Park, Ascot, Berks SL5 7PY 2Entomology and Nematology Department, AFRC IACR, Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ
R. N. Perry
Affiliation:
2Entomology and Nematology Department, AFRC IACR, Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ
D. J. Wright
Affiliation:
1Department of Biology, Imperial College, Silwood Park, Ascot, Berks SL5 7PY
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Summary

Indirect immunofluorescent studies have been used to localize a glycoprotein (gp32) in the amphids of 2nd-stage juveniles (J2) of Meloidogyne incognita parasitic in the roots of tomato plants, male Meloidogyne javanica and some young adult female M. incognita. Immuno-electron microscopic studies showed an apparent absence of gp32 in the amphids of mature adult female M. incognita. Chemoattraction experiments demonstrated that the binding of gp32 antibodies to the amphidial secretions of infective J2 M. incognita partly impaired the ability of the nematodes to locate tomato roots.

Keywords

plant-parasitic nematodesMeloidogyne incognitaamphidssense organsimmunocytochemistrychemoattraction
Type
Research Article
Information
Parasitology , Volume 107 , Issue 5 , December 1993 , pp. 573 - 578
Copyright
Copyright © Cambridge University Press 1993

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References

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