Hostname: page-component-76fb5796d-5g6vh Total loading time: 0 Render date: 2024-04-28T09:09:33.899Z Has data issue: false hasContentIssue false
  • English
  • Français

Studies of the amino acids of Rhodnius prolixus. I. Analysis of the haemolymph

Published online by Cambridge University Press:  06 April 2009

J. S. Harington
J. S. Harington
Affiliation:
Department of Entomology, London School of Hygiene and Tropical Medicine*
Get access Rights & Permissions [Opens in a new window]

Extract

1. A qualitative and semi-quantitative analysis of the free amino acids in the haemolymph of Rhodnius prolixus has been made by paper chromatography.

2. Twenty-one amino acids or related compounds were detected of which histidine appears at the highest concentration.

3. The α-amino-N concentration of haemolymph is among the lowest values recorded for insect haemolymphs.

4. A substance corresponding in position to thyroxine has been detected in haemolymph of a few immature insects, and is formed if iodate is added to freshly withdrawn haemolymph.

I wish to record my great appreciation to the late Professor P. A. Buxton, C.M.G., F.R.S., for his valuable assistance and criticism during this study and to members of the staff for helpful discussions. The study forms part of a Thesis for the degree of Ph.D. approved by the University of London, and was financed by the South African Council for Scientific and Industrial Research whose generous support I acknowledge with pleasure.

Type
Research Article
Information
Parasitology , Volume 51 , Issue 3-4 , November 1961 , pp. 309 - 318
Copyright
Copyright © Cambridge University Press 1961

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Albritton, E. C. (1954) (editor). Standard Values in Blood. London: W. B. Saunders Co.Google Scholar
Auclair, J. L. & Dubreuil, R. (1952). A simple ultra-micromethod for the quantitative estimation of amino acids by paper partition chromatography. Canad. J. Zool. 30, 109.CrossRefGoogle Scholar
Auclair, J. L. & Dubreuil, R. (1953). Étude sur les acides aminés libres d'hémolymphe des Insectes par le méthode de chromatographie sur papier filtre. Canad. J. Zool. 31, 30.CrossRefGoogle Scholar
Barbour, H. G. & Hamilton, W. F. (1926). The falling drop method for determining specific gravity. J. Biol. Chem. 69, 625.CrossRefGoogle Scholar
Beadle, L. C. & Shaw, J. (1950). The retention of salt and the regulation of the non-protein nitrogen fraction in the blood of the aquatic larva, Sialis lutaria. J. Exp. Biol. 27, 96.CrossRefGoogle Scholar
Bishop, G. H., Briggs, A. P. & Ronzoni, E. (1925). Body fluids of the honey bee larva. J. Biol. Chem. 66, 77.CrossRefGoogle Scholar
Buxton, P. A. (1930). The biology of the blood-sucking bug, Rhodnius prolixus. Trans. Ent. Soc. Lond. 78, 227.CrossRefGoogle Scholar
Chen, P. S. & Hadorn, E. (1954). Vergleichende Untersuchungen uber die freien Amino sauren in der larvalen Haemolymphe von Drosophila, Ephestia und Corethra. Rev. suisse Zool. 61, 437.CrossRefGoogle Scholar
Consden, R., Gordon, A. H. & Martin, A. J. P. (1944). Qualitative analysis of proteins: a partition chromatographic method using paper. Biochem. J. 38, 224.CrossRefGoogle ScholarPubMed
Datta, S. P., Dent, C. E. & Harris, H. (1950). An apparatus for the simultaneous production of many two-dimensional paper chromatograms. Science, 112, 621.CrossRefGoogle ScholarPubMed
Dent, C. E. (1948). A study of the behaviour of some sixty amino acids and other ninhydrinreacting substances on phenol- ‘collidine' filter-paper chromatograms, with notes as to the occurrence of some of them in biological fluids. Biochem. J. 43, 169.CrossRefGoogle Scholar
Dubreuil, R. M., Auclair, J. L. & Timiras, P. S. (1953). Comparative study of the free amino acids in the serum of four mammals by paper partition chromatography. Rev. canad. Biol. 11, 401.Google Scholar
Duchateau, G. & Florkin, M. (1953). Teneur en acides aminés non protéinique du plasma de l'hémolymphe des Chenilles de Cossus cossus et de deux Saturnides africains Imbrasia macrothyris et Pseudobunaea seydeli. Arch. int. Physiol. 61, 232.Google Scholar
Duchateau, G., Florkin, M. & Sarlet, H. (1952). Sur les acides aminés, libres ou combinés sous forme non protéinique, du plasma sanguin de divers insectes. Arch. int. Physiol. 60, 539.Google Scholar
Duchateau, G., Sarlet, H. & Florkin, M. (1952). Sur les acides aminés, libres ou combines sous forme non protéinique du plasma sanguin de differents insectes (Phasme, larve d'Abeille, Lépidoptères). Arch. int. Physiol. 60, 103.Google Scholar
Florkin, M. (1937). Contributions à l'étude du plasma sanguin des insectes. Mém. Acad. R. Belg. (Cl. Sci.), 16, 1.Google Scholar
Florkin, M. (1949). Biochemical Evolution. New York: Academic Press.Google Scholar
Fukuda, T. & Sakano, S. (1950). Discrimination by paper chromatography of the free amino acids in the blood of silkworm pupa. J. seric. Sci., Tokyo, 19, 490.Google Scholar
Hamilton, P. B. & van Slyke, D. D. (1943). The gasometric determination of free amino acids in blood filtrates by the ninhydrin-carbon dioxide method. J. Biol. Chem. 150, 231.CrossRefGoogle Scholar
Harington, C. R. (1944). Thyroxine: its biosynthesis and its immunochemistry. Proc. Roy. Soc. B, 132, 223.Google Scholar
Harington, J. S. (1956). Histamine and histidine in excreta of the blood-sucking bug, Rhodnius prolixus. Nature, Lond., 178, 268.CrossRefGoogle ScholarPubMed
Harrow, B. (1946). Textbook of Biochemistry, 4th ed. London: W. B. Saunders Co.Google Scholar
Hinton, H. E. (1954). Insect blood. Sci. Progr., 42, 684.Google Scholar
Levenbook, L. (1950). The composition of horse bot fly (Gastrophilus intestinalis) larval blood. Biochem. J. 47, 336.CrossRefGoogle Scholar
Richardson, C. H., Burdette, R. C. & Eagleson, C. W. (1931). The determination of the blood volume of insect larvae. Ann. Ent. Soc. Amer. 24, 503.CrossRefGoogle Scholar
Roche, J. & Michel, R. (1951). Natural and artificial iodoprotein. Advanc. Protein Chem. 6, 253.CrossRefGoogle Scholar
Roeder, K. (1953) (editor). Insect Physiology. London: Chapman and Hall.Google Scholar
Saifer, A. & Oreskes, I. (1953). Circular paper chromatography. I. Studies of physical factors that may influence RF values. Analyt. Chem. 25, 1539.CrossRefGoogle Scholar
Stein, W. H. & Moore, S. (1954). The free amino acids of human plasma. J. Biol. Chem. 24, 915.CrossRefGoogle Scholar
Talbot, N. B., Butler, A. M., Saltzman, A. H. & Rodriguez, P. M. (1944). The colorimetric estimation of protein-bound serum iodine. J. Biol. Chem. 153, 479.CrossRefGoogle Scholar
Taurog, A. & Chaikoff, I. L. (1946). On the determination of plasma iodine. J. Biol. Chem. 163, 313.CrossRefGoogle ScholarPubMed
Taurog, A. & Chaikoff, I. L. (1948). The nature of the circulating thyroid hormone. J. Biol. Chem. 176, 639.CrossRefGoogle ScholarPubMed
Ussing, H. H. (1946). Amino acids and related compounds in the haemolymph of Oryctes nasicornis and Melolontha vulgaris. Acta physiol. scand. 11, 61.CrossRefGoogle Scholar
van Slyke, D. D., MacFadyen, D. A. & Hamilton, P. (1941). Determination of free amino acids by titration of the carbon dioxide formed in the reaction with ninhydrin. J. Biol. Chem. 141, 671.CrossRefGoogle Scholar
Westall, R. G. (1948). Note on the behaviour of inorganic salts on the filter-paper partition chromatogram. Biochem. J. 42, 249.Google ScholarPubMed
Winegard, H. M., Toennies, G. & Block, R. J. (1948). Detection of sulphur-containing amino acids on paper chromatograms. Science, 108, 506.CrossRefGoogle Scholar
Work, E. (1949). Chromatographic investigations of amino acids from micro-organisms. Biochem. Biophys. Acta, 3, 400.CrossRefGoogle Scholar