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Scanning electron microscopy of Quilonia renniei from Asian elephants revealing variation in coronal leaflet number

Published online by Cambridge University Press:  13 December 2021

Hla Myet Chel ,
Saw Bawm Open the ORCID record for Saw Bawm [Opens in a new window] ,
Lat Lat Htun ,
Md. Abdul Masum ,
Osamu Ichii ,
Nariaki Nonaka ,
Ryo Nakao  and
Ken Katakura Open the ORCID record for Ken Katakura [Opens in a new window]
Hla Myet Chel
Affiliation:
Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw 15013, Myanmar
Saw Bawm
Affiliation:
Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw 15013, Myanmar Department of International Relations and Information Technology, University of Veterinary Science, Yezin, Nay Pyi Taw 15013, Myanmar
Lat Lat Htun
Affiliation:
Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw 15013, Myanmar
Md. Abdul Masum
Affiliation:
Laboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
Osamu Ichii
Affiliation:
Laboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan Laboratory of Agrobiomedical Science, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
Nariaki Nonaka
Affiliation:
Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
Ryo Nakao
Affiliation:
Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
Ken Katakura*
Affiliation:
Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
*
Author for correspondence: Ken Katakura, E-mail: kenkata@vetmed.hokudai.ac.jp
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Abstract

Although parasitic nematodes in the genera Murshidia and Quilonia (family Strongylidae) are recognized as major gastrointestinal parasites in Asian elephants, they have been poorly studied. Recently, light micrographs of these parasites in Myanmar have been presented, almost 100 years after the original drawings. However, the number of coronal leaflets, a key taxonomic feature of Quilonia species, has not been precisely determined based on light microscopy. The current study aimed to determine the exact number of coronal leaflets in Quilonia renniei specimens from Asian elephants in Myanmar. On the basis of scanning electron micrographs, leaflet number in females (19–20, average 19.7, n = 9) was significantly higher (P < 0.005) than that in males (16–19, average 18.1, n = 8). This compares with 18 coronal leaflets indicated in the original species description. Specimens bearing 19 coronal leaflets were most numerous, followed by those with 20 leaflets. Median-joining network analysis of mitochondrial cytochrome c oxidase subunit I gene sequences with 16 haplotypes from 19 individuals revealed no clear association between parasite populations and the number of coronal leaflets. These results highlight the importance of determining the number of coronal leaflets in the taxonomy of Q. renniei and other related Quilonia species infecting Asian elephants.

Keywords

Asian elephantcoronal leafletmedian-joining networkMyanmarQuilonia rennieiscanning electron microscopytaxonomy
Type
Research Article
Information
Parasitology , Volume 149 , Issue 4 , April 2022 , pp. 529 - 533
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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