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The rapid development of the glucose transport system in the excysted metacestode of Hymenolepis diminuta

Published online by Cambridge University Press:  06 April 2009

R. Rosen ,
M. L. San ,
M. E. Denton ,
J. M. Wolfe  and
G. L. Uglem
R. Rosen
Affiliation:
Department of Biology, Berea College, Berea, Kentucky 40404, USA
M. L. San
Affiliation:
Department of Biology, Berea College, Berea, Kentucky 40404, USA
M. E. Denton
Affiliation:
Department of Biology, Berea College, Berea, Kentucky 40404, USA
J. M. Wolfe
Affiliation:
Department of Biology, Berea College, Berea, Kentucky 40404, USA
G. L. Uglem
Affiliation:
Department of Biology, Berea College, Berea, Kentucky 40404, USA
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Summary

Temporal changes in glucose transport capacity in excysted scoleces of Hymenolepis diminuta were examined. Assays involved incubation for 1 min in [3H]glucose after pre-incubation for 1 min to 8 h in saline. There were two abrupt increases in uptake velocity, a relatively small one between 15 and 75 min, and a large one between 5 and 6 h, during which the Vmax increased from 0·36 to 2·49 nmol/25 larvae/h. The second increase was unaffected when the pre-incubation saline contained 5 mM glucose, but it was completely blocked when the excysted larvae were pre-incubated in Ca2+-free saline. Abrupt glucose transport changes did not occur in intact cysticercoids or in scoleces when the substrate was [3H]leucine or [3H]uracil. Arrhenius plots (log V versus 1/temperature, 10–42 °C) were linear for intact cysticercoids, but were biphasic for both scoleces and adults with discontinuities at 20 ± 1 °C. Thus, ‘activation’ of the excysted scolex seemed to involve a specific, Ca2+ -dependent increase in number of glucose transporters functioning in the worm surface. The Arrhenius plots indicated that development in the final host does not involve a major change in lipid composition of the parasite's membranes.

Keywords

tapewormHymenolepis diminutaglucose transport
Type
Research Article
Information
Parasitology , Volume 108 , Issue 2 , February 1994 , pp. 217 - 222
Copyright
Copyright © Cambridge University Press 1994

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References

REFERENCES

Arme, C. (1988). Ontogenetic changes in helminth membrane function. Parasitology 96, S83–S104.CrossRefGoogle ScholarPubMed
Barrett, J. (1987). Developmental aspects of metabolism in parasites. International Journal for Parasitology 17, 105–10.CrossRefGoogle ScholarPubMed
Fairbairn, D., Wertheim, G., Harpaur, R. R. & Schiller, E. L. (1961). Biochemistry of normal and irradiated strains of Hymenolepis diminuta. Experimental Parasitology 11, 248–63.CrossRefGoogle ScholarPubMed
Harris, B. G. & Read, C. P. (1969). Factors affecting protein synthesis in Hymenolepis diminuta (Cestoda). Comparative Biochemistry and Physiology 28, 645–54.CrossRefGoogle ScholarPubMed
Jeffs, S. A. & Arme, C. (1984). Hymenolepis diminuta. protein synthesis in cysticercoids. Parasitology 88, 351–7.CrossRefGoogle Scholar
Neame, K. D. & Richards, T. G. (1972). Elementary Kinetics of Membrane Carrier Transport. New York: John Wiley.Google Scholar
Papahadjopoulos, D., Nir, S. & Düzgünes, N. (1990). Molecular mechanisms of calcium-induced membrane fusion. Journal of Bioenergetics and Biomembranes 22, 157–79.CrossRefGoogle ScholarPubMed
Pappas, P. W. & Read, C. P. (1975). Membrane transport in helminth parasites: a review. Experimental Parasitology 37, 469–530.CrossRefGoogle ScholarPubMed
Pappas, P. W., Uglem, G. L. & Read, C. P. (1973). The influx of purines and pyrimidines across the brush border of Hymenolepis diminuta. Parasitology 66, 525–38.CrossRefGoogle ScholarPubMed
Pollard, H. B., Burns, A. L. & Rojas, E. (1990). Synexin (annexin VII): A cytosolic calcium-binding protein which promotes membrane fusion and forms calcium channels in artificial bilayer and natural membranes. Journal of Membrane Biology 117, 101–12.CrossRefGoogle ScholarPubMed
Prescott, L. M. & Campbell, J. W. (1965). Phosphoenolpyruvate carboxylase activity and glycogenesis in the flatworm Hymenolepis diminuta. Comparative Biochemistry and Physiology 14, 491–511.CrossRefGoogle ScholarPubMed
Read, C. P., Rothman, A. H. & Simmons, J. E. Jr. (1963). Studies on membrane transport, with special reference to parasite–host integration. Annals of the New York Academy of Sciences 113, 154–205.CrossRefGoogle ScholarPubMed
Richards, K. S. & Arme, C. (1984). Maturation of the scolex syncytium in the metacestode of Hymenolepis diminuta, with special reference to microthrix formation. Parasitology 88, 341–9.CrossRefGoogle Scholar
Roberts, L. S. (1983). Carbohydrate metabolism. In Biology of the Eucestoda, Vol. 2 (ed. Arme, C. & Pappas, P. W. ), pp. 343–90. London: Academic Press.Google Scholar
Rosen, R. & Uglem, G. L. (1988). Localization of facilitated diffusion and active glucose transport in cysticercoids of Hymenolepis diminuta (Cestoda). International Journal for Parasitology 18, 581–4.CrossRefGoogle ScholarPubMed
Rothman, A. H. (1959). Studies on excystment of tapeworms. Experimental Parasitology 8, 336–64.CrossRefGoogle ScholarPubMed
Uglem, G. L., Pappas, P. W. & Read, C. P. (1973). Surface aminopeptidase in Moniliformis dubius and its relation to amino acid uptake. Parasitology 67, 185–95.CrossRefGoogle ScholarPubMed
Uglem, G. L., Dupre, R. K. & Harley, J. P. (1983). Allosteric control of pyrimidine transport inHymenolepis diminuta: an unusual kinetic isotope effect. Parasitology 87, 289–93.CrossRefGoogle ScholarPubMed
Walker, R. W. & Barrett, J. (1983). Mitochondrial adenosine triphosphatase activity and temperature adaptation Schistocephalus solidus (Cestoda: Pseudophyllidea). Parasitology 87, 307–26.CrossRefGoogle Scholar
Walker, R. W. & Barrett, J. (1985). Mitochondrial membrane fluorescence and temperature adaptation in Schistocephalus solidus (Cestoda: Pseudophyllidea). Parasitology 90, 131–5.CrossRefGoogle Scholar
Yinon, U. & Shulov, A. (1970). The dispersion of Trogoderma granarium in a temperature gradient and comparison with other stored product beetles. Entomologia Experimentalis et Applicata 13, 107–21.CrossRefGoogle Scholar