Hostname: page-component-7479d7b7d-t6hkb Total loading time: 0 Render date: 2024-07-10T19:56:53.276Z Has data issue: false hasContentIssue false

Pyrolysis gas-liquid chromatography of the pathotypes of the potato cyst nematodes Globodera rostochiensis and G. pallida

Published online by Cambridge University Press:  06 April 2009

P. C. Fox
Affiliation:
Department of pure and Applied ZoolOgy, The University of Leeds, Leeds LS2 9JT
H. J. Atkinson
Affiliation:
Department of pure and Applied ZoolOgy, The University of Leeds, Leeds LS2 9JT

Summary

Curie-point pyrolysis gas-liquid chromatography (PGLC) was used to detect gross chemical differences between the 8 European pathotypes of the potato cyst nematodes Globodera rostochiensis and G. pallida. No qualitative differences were seen with either flame ionization or flame photometric detection systems, although quantitative differences between pathotypes were detected. Cluster analysis of these differences failed to group the pathotypes into species, but separation of some economically important pathotypes was achieved. The potential of PGLC for nematode identification is discussed.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abbey, L. E., Highsmith, A. K., Moran, R. F. & Reiner, E. J. (1981). Differentiation and Characterization of klebsiella pneumoniae strains by pyrolysis-gas-liquid chromatography-mass spectrometry. Journal of Clinical Microbiology 13, 313–19.CrossRefGoogle ScholarPubMed
Ballantyne, A. J. (1980). Physiological aspects of the hatching process in the phytoparasitic nematode Globodera rostochiensis. Ph.D. thesis, University of Leeds.Google Scholar
Cone, R. D. & Lechowich, R. V. (1970) Differentiation of Clostridium botulinum types A, B and E by pyrolysis gas-liquid chromatography. Applied Microbiology 19, 138–45.CrossRefGoogle Scholar
Emswiler, B. S. & Kotula, W. A. (1978). Differentiation of Salmonella serotypes by pyrolysis gas-liquid chromatography. Applied and Environmental Microbiology 35, 97104.CrossRefGoogle ScholarPubMed
Fox, P. C. & Atkinson, H. J. (1984 a). Isoelectric focusing of general protein and specific enzymes from pathotypes of Globodera rostochiensis and G. pallida. Parasitology 88, 131–9.CrossRefGoogle Scholar
Fox, P. C. & Atkinson, H. J. (1984 b). Glucose phosphate isomerase polymorphism in field populations Of the potato cyst nematodes, Globodera rostochiensis and G. pallida. Annals of Applied Biology 104, 503–9.CrossRefGoogle Scholar
Fox, P. C. & Atkinson, H. J. (1985). Immunochemical studies on pathotypes of the potato cyst nematodes, Globodera rostochiensis and G. pallida. Parasitology 90, 471–83.CrossRefGoogle Scholar
Gutteridge, C. S. & Norris, J. R. (1979). The application of pyrolysis techniques to the identification Of micro-organisms. Journal of Applied Bacteriology 47, 543.CrossRefGoogle Scholar
Haddadin, J. M., Stirland, R. M., Preston, N. W. & Collard, P. (1973). Identification of Vibrio choleras by pyrolysis gas-liquid chromatography. Applied Microbiology 25, 40–3.CrossRefGoogle ScholarPubMed
Hall, R. C. & Bennett, G. W. (1973). Pyrolysis gas chromatography of several cockroach species. Journal of Chromatographic Science 11, 439–43.CrossRefGoogle ScholarPubMed
Hansen, e. L. & Buecher, E. J. (1970). Biochemical approach to systematic studies with axenic nematodes. Journal of Nematology 2, 16.Google ScholarPubMed
Huis in't Veld, H. J. H., Meuzelaar, H. L. C. & Tom, A. (1973) Analysis of streptococcal cell wall fractions by Curie-point pyrolysis gas-liquid chromatography. Applied Microbiology 26, 92–7.CrossRefGoogle Scholar
Hussey, R. S. (1979). Biochemical systematics of nematodes – a review. Helminthological Abstracts, Series B, Plant Nematology 48, 141–8.Google Scholar
Jones, F. G. W. (1975). Host parasite relationships of potato cyst nematodes: a speculation arising from the gene-for-gene hypothesis. Nematologica 20, 437–43.CrossRefGoogle Scholar
Keen, N. T. (1982). Specific recognition in gene-for-gene host-parasite systems. In Advances in Plant Pathology, vol. 1, (ed. Ingram, D. S. and Williams, P. H.), pp. 3592. London: Academic Press.Google Scholar
Kort, J., Ross, H., Rumpenhorst, H. J. & Stone, A. R. (1977). An international scheme for identifying and classifying pathotypes of potato cyst-nematodes G. rostochiensis and G. pallida. Nematologica 23, 333–9.CrossRefGoogle Scholar
Krusberg, L. R., Hussey, R. S. & Fletcher, C. L. (1973). Lipid and fatty acid composition of females and eggs of Meloidogyne incognita and M. arenaria. Comparative Biochemistry and Physiology 45B, 335–41.Google Scholar
Levy, R. L., Fantner, D. L. & Wolf, C. J. (1972). Temperature rise time and true pyrolysis temperature in pulse mode pyrolysis gas chromatography. Analytical Chemistry 44, 3842.CrossRefGoogle Scholar
Meuzelaar, H. L. C. & In't Veld, R. A. (1972). A technique for Curie-point pyrolysis gas chromatography of complex biological samples. Journal of Chromatographic Science 10, 213–16.CrossRefGoogle ScholarPubMed
Myers, A. & Smith, R. N. L. (1972). Application of pyrolysis gas chromatography to biological materials. Chromatographia 5, 521–4.CrossRefGoogle Scholar
Quinn, P. A. (1974). Development of high resolution pyrolysis gas chromatography for the identification of micro-organisms. Journal of Chromatographic Science 12, 796806.CrossRefGoogle Scholar
Reiner, E. (1967). Studies on differentiation of micro-organisms by pyrolysis gas-liquid chromatography. Journal of Gas Chromatography 5, 65–7.CrossRefGoogle Scholar
Stone, A. R. & Valenzuela, A. (1979). Pathotypes of Globodera rostochiensis present in the United Kingdom. Nematologica 25, 3745.Google Scholar
Storey, R. M. J. & Atkinson, H. J. (1982). Factors influencing the depletion of neutral lipid reserves of field populations of Globodera spp. Nematologica 28, 175.Google Scholar
Turner, S. J., Stone, A. R. & Perry, J. N. (1983). Selection of potato cyst-nematodes on resistant Solanum vernei hybrids. Euphytica 32, 911–17.CrossRefGoogle Scholar
Vincent, P. G. & Kulik, M. M. (1970). Pyrolysis gas-liquid chromatography of fungi: differentiation of species and strains of several members of the Aspergillus flavus group. Applied Microbiology 20, 957–63.CrossRefGoogle ScholarPubMed
Weijman, A. C. M. (1977). The application of Curie-point pyrolysis mass spectrometry in fungal taxonomy. In Analytical Pyrolysis (ed. Jones, C. E. R. and Cramer, C. A.), pp. 225–33. Amsterdam: Elsevier.CrossRefGoogle Scholar
Weir, D. M. (1978). Handbook of Experimental Immunology. Oxford: Blackwell.Google Scholar
Wharton, R. J., Storey, R. M. J. & Fox, P. C. (1983). The potential of some immunochemical and biochemical approaches to the taxonomy of potato cyst-nematodes. In Concepts in Nematode Systematics (ed. Stone, A. R., Platt, H. M. and Khalil, L. F.), pp. 235–48. London and New York: Academic Press.Google Scholar