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Purified preparations of tobacco necrosis virus (Nicotiana virus II)

Published online by Cambridge University Press:  06 April 2009

N. W. Pirie ,
K. M. Smith ,
E. T. C. Spooner  and
W. D. McClement
N. W. Pirie
Affiliation:
Biochemical Laboratory, Cambridge.
K. M. Smith
Affiliation:
Potato Virus Research Station and Molteno Institute, Cambridge.
E. T. C. Spooner
Affiliation:
Department of Pathology, Cambridge.
W. D. McClement
Affiliation:
Potato Virus Research Station and Molteno Institute, Cambridge.
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Extract

Two nucleoproteins with similar chemical composition have been isolated from the leaves of tobacco plants infected with tobacco necrosis virus. One of these is crystalline and has a sedimentation constant of 130 × 10−13; the other is amorphous and its principal component has a sedimentation constant of 58 × lO−13.

Each preparation will infect plants at a dilution of 1 in 108 and will precipitate specifically with antiserum at a dilution of 1 in 3·2 × 105.

The nature of the difference between preparations in the two states is obscure and it has not proved possible to convert the one into the other.

Type
Research Article
Information
Parasitology , Volume 30 , Issue 4 , December 1938 , pp. 543 - 551
Copyright
Copyright © Cambridge University Press 1938

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References

REFERENCES

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