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Proteinases in the excretory/secretory products (ES) of adult Trichinella spiralis

Published online by Cambridge University Press:  06 April 2009

V. K. Todorova ,
D. P. Knox  and
M. W. Kennedy
V. K. Todorova
Affiliation:
Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Bearsden, Glasgow
D. P. Knox
Affiliation:
Moredun Research Institute, 408 Gilmerton Road, Edinburgh
M. W. Kennedy
Affiliation:
Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Bearsden, Glasgow
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Summary

Adult Trichinella spiralis were maintained in vitro using defined media and the material excreted/secreted (ES) during this time examined for proteolytic enzyme (proteinase) activity using an azocasein assay and gelatin-substrate gels. Several discrete proteinases in the size range 14–100 kDa were observed with optimal activity at pH 7·5. The use of a class-differentiating panel of proteinase inhibitors indicated that serine proteinases were predominant although some inhibition was evident in the presence of cysteine and metalloproteinase inhibitors. Of a panel of potential natural protein substrates tested, ES proteinases only degraded fibrinogen and plasminogen and degradation was, in part, susceptible to the action of serine, cysteine and aspartyl proteinase inhibitors. In addition, antibody harvested from immune but not normal mice inhibited ES proteinase activity, an observation of relevance to the immunobiology of Trichinosis.

Keywords

Trichinella spiralisexcretory/secretoryproteolytic enzymescharacterizationantibody inhibition
Type
Research Article
Information
Parasitology , Volume 111 , Issue 2 , August 1995 , pp. 201 - 208
Copyright
Copyright © Cambridge University Press 1995

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