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Phylogenetic taxonomy of Leishmania (Viannia) braziliensis based on isoenzymatic study of 137 isolates

Published online by Cambridge University Press:  01 October 1997

N. CHOUICHA
Affiliation:
Laboratoire d'Ecologie Médicale et Pathologie Parasitaire, Faculté de Médecine, 163 rue Auguste Broussonet, Montpellier 34090, France
G. LANOTTE
Affiliation:
Laboratoire d'Ecologie Médicale et Pathologie Parasitaire, Faculté de Médecine, 163 rue Auguste Broussonet, Montpellier 34090, France
F. PRATLONG
Affiliation:
Laboratoire d'Ecologie Médicale et Pathologie Parasitaire, Faculté de Médecine, 163 rue Auguste Broussonet, Montpellier 34090, France
C. A. CUBA CUBA
Affiliation:
Departamento de Patologia, Facultade de Ciencias da Saúde, Universidade de Brasilia, Brasilia DF70910, Brasil
I. D. VELEZ
Affiliation:
Laboratorio de Leishmaniose, Universidad de Antioquia, Medellin, Colombia
J. P. DEDET
Affiliation:
Laboratoire d'Ecologie Médicale et Pathologie Parasitaire, Faculté de Médecine, 163 rue Auguste Broussonet, Montpellier 34090, France

Abstract

Biochemical characterization of 137 Leishmania braziliensis isolates from South and Central America, and from selected endemic foci in Bolivia, Brazil and Colombia, performed by isoenzymatic electrophoresis using 10 enzymatic systems, showed a high enzymatic polymorphism (44 zymodemes obtained) based on the variation of a small number of enzymes. Cladistic analysis showed close links between the zymodemes within the L. braziliensis s.s. cluster. The position of 2 Colombian zymodemes obtained (MON*204 and MON*205) justify the inclusion of L. peruviana within the L. braziliensis cluster.

Type
Research Article
Copyright
1997 Cambridge University Press

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