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A new microsporidian parasite, Heterosporis saurida n. sp. (Microsporidia) infecting the lizardfish, Saurida undosquamis from the Arabian Gulf, Saudi Arabia: ultrastructure and phylogeny

Published online by Cambridge University Press:  08 February 2012

S. AL-QURAISHY ,
A. S. ABDEL-BAKI ,
H. AL-QAHTANI ,
M. DKHIL ,
G. CASAL  and
C. AZEVEDO
S. AL-QURAISHY
Affiliation:
Zoology Department, College of Science, King Saud University, P.O. Box 12455, Riyadh 11451, Saudi Arabia
A. S. ABDEL-BAKI*
Affiliation:
Zoology Department, College of Science, King Saud University, P.O. Box 12455, Riyadh 11451, Saudi Arabia Zoology Department, Faculty of Science, Beni-Suef University, Egypt
H. AL-QAHTANI
Affiliation:
Zoology Department, College of Science, King Saud University, P.O. Box 12455, Riyadh 11451, Saudi Arabia
M. DKHIL
Affiliation:
Zoology Department, College of Science, King Saud University, P.O. Box 12455, Riyadh 11451, Saudi Arabia Department of Zoology and Entomology, Faculty of Science, Helwan University, Egypt
G. CASAL
Affiliation:
Department of Cell Biology, Institute of Biomedical Sciences, University of Porto (ICBAS/UP), 4099–003 Porto, Portugal Laboratory of Pathology, Interdisciplinary Centre of Marine Environmental Research, University of Porto (CIIMAR/UP), Porto, Portugal Departamento de Ciências, Instituto Superior de Ciências da Saúde – Norte, CESPU, Gandra, Portugal
C. AZEVEDO
Affiliation:
Zoology Department, College of Science, King Saud University, P.O. Box 12455, Riyadh 11451, Saudi Arabia Department of Cell Biology, Institute of Biomedical Sciences, University of Porto (ICBAS/UP), 4099–003 Porto, Portugal Laboratory of Pathology, Interdisciplinary Centre of Marine Environmental Research, University of Porto (CIIMAR/UP), Porto, Portugal
*
*Corresponding author: Zoology Department, College of Science, King Saud University, P.O. Box 12455, Riyadh 11451, Saudi Arabi. Tel: +0096614675754. Fax: +0096614678514. E-mail: azema1@yahoo.com
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Summary

A new microsporidian that infects the lizardfish Saurida undosquamis (Richardson, 1848) that are caught in the Arabian Gulf in Saudi Arabia is described here. This parasite invades the skeletal muscle of the abdominal cavity forming white, cyst-like structures containing numerous spores. The prevalence of the infection was 32·1% (135/420). The spores were oval to pyriform in shape and measured approximately 3·3 μm×2·0 μm. The developing spores were found within parasitophorous vacuoles. In mature spores, the polar filament was arranged into 5 coils in a row. Molecular analysis of the rRNA genes, including the ITS region, and phylogenetic analyses using maximum parsimony, maximum likelihood, and Bayesian inference were performed. The ultrastructural characteristics and phylogenetic analyses support the recognition of a new species, herein named Heterosporis saurida n. sp.

Keywords

Arabian Gulf fishMicrosporidiaultrastructurephylogenyHeterosporis saurida
Type
Research Article
Information
Parasitology , Volume 139 , Issue 4 , April 2012 , pp. 454 - 462
Copyright
Copyright © Cambridge University Press 2012

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References

REFERENCES

Coste, F. and Bouix, G. (1998). Heterosporis cichlidarum n. sp. (Microspora), a parasite of the ornamental cichlid fish Hemichromis bimaculatus Gill, 1862. Bulletin of the European Association of Fish Pathologists 18, 157–161.Google Scholar
Diamant, A., Goren, M., Yokes, M. B., Galil, B. S., Klopman, Y., Huchon, D., Szitenberg, A. and Karhan, S. Ü. (2010). Dasyatispora levantinae gen. et sp. nov., a new microsporidian parasite from the common stingray Dasyatis pastinaca in the eastern Mediterranean. Diseases of Aquatic Organisms 91, 137–150.CrossRefGoogle Scholar
Dyková, I. (1995). Phylum Microspora. In Fish Diseases and Disorders, Vol. 1. Protozoan and Metazoan Infections (ed. Woo, P. T. K.), pp. 149–179. CAB International, Cambridge, UK.Google Scholar
Gatehouse, H. S. and Malone, L. A. (1998). The ribosomal RNA gene region of Nosema apis (Microspora): DNA sequence for small and large subunit rRNA genes and evidence of a large tandem repeat unit size. Journal of Invertebrate Pathology 71, 97–105.Google Scholar
Hoshina, T. (1951). On a new microsporidian, Pleistophora anguillarum n. sp. from the muscle of the eel Anguilla japonica. Journal of the Tokyo University of Fisheries 38, 35–46.Google Scholar
Joh, S. J., Kwon, Y. K., Kim, M. C., Kim, M. J., Kwon, H. M., Park, J. W., Kwon, J. H. and Kim, J. H. (2007). Heterosporis anguillarum infections in farm cultured eels (Anguilla japonica) in Korea. Journal of Veterinary Sciences 8, 147–149.Google Scholar
Larsson, J. I. R. (1999). Identification of Microsporidia. Acta Protozoologica 38, 161–197.Google Scholar
Lom, J., Dyková, I., Körting, W. and Klinger, H. (1989). Heterosporis schuberti n. sp., a new microsporidian parasite of aquarium fish. European Journal of Protistology 25, 129–135.CrossRefGoogle Scholar
Lom, J., Dyková, I., Wang, C. H., Lo, C. F. and Kou, G. H. (2000). Ultrastructural justification for the transfer of Pleistophora anguillarum Hoshina, 1959 to the genus Heterosporis Schubert, 1969. Diseases of Aquatic Organisms 43, 225–231.CrossRefGoogle Scholar
Lom, J. and Nilsen, F. (2003). Fish microsporidia: fine structural diversity and phylogeny. Intentional Journal of Parasitology 33, 107–127.Google Scholar
Michel, C., Maurand, J., Loubes, C., Chilmonczyk, S. and de Kinkelin, P. (1989). Heterosporis finki, a microsporidian parasite of the angel fish Pterophyllum scalare: pathology and ultrastructure. Diseases of Aquatic Organisms 7, 103–109.Google Scholar
Negm-Eldin, M. M. (1992). On two new species of Pleistophora (Microsporidea) from the Egyptian fishes. Egyptian Journal of Applied Sciences 7, 469–478.Google Scholar
Pekkarinen, M., Lom, J. and Nilsen, F. (2002). Ovipleistophora gen. n., a new genus for Pleistophora mirandellae-like microsporidia. Diseases of Aquatic Organisms 48, 133–142.Google Scholar
Peyghan, R., Nabavi, L., Jamshidi, K. and Akbari, S. (2009). Microsporidian infection in lizardfish, Saurida undosquamis of Persian Gulf. Iranian Journal of Veterinary Research 10, 180–185.Google Scholar
Ronquist, F. and Huelsenbeck, J. P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.Google Scholar
Sanders, J. L., Lawrence, C., Nichols, D. K., Brubaker, J. F., Peterson, T. S., Murray, K. N. and Kent, M. L. (2010). Pleistophora hyphessobryconis (Microsporidia) infecting zebrafish Danio rerio in research facilities. Diseases of Aquatic Organisms 91, 47–56.Google Scholar
Schubert, G. (1969). Ultracytologische Untersuchungen an der spore des Mikrosporidienart, Heterosporis finki gen. n., sp. n. Zeitschrift für Parasitenkunde 32, 59–79.Google Scholar
Sutherland, D., Marcquenski, S., Marcino, J., Lom, J., Dyková, I., Hsu, H. M., Jahns, W. and Nilsen, F. (2000). Heterosporis sp. (Microspora: Glugeidae): a new parasite from Perca flavescens in Wisconsin and Minnesota. Proceedings of the 62nd Midwest Fish & Wildlife Conference, Minneapolis, MN, December 3–6.Google Scholar
Tamura, K., Dudley, J., Nei, M. and Kumar, S. (2007). MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology Evolution 24, 1596–1599.Google Scholar
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution (in the Press) doi: 10.1093/molbev/msr121.CrossRefGoogle ScholarPubMed
Thompson, J. D., Higgins, D. G. and Gilson, T. J. (1994). Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673–4680.Google Scholar