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New insights on the genetic diversity of the honeybee parasite Nosema ceranae based on multilocus sequence analysis

Published online by Cambridge University Press:  23 July 2013

MATHIEU ROUDEL ,
JULIE AUFAUVRE ,
BRUNO CORBARA ,
FREDERIC DELBAC  and
NICOLAS BLOT
MATHIEU ROUDEL
Affiliation:
Clermont Université, Université Blaise Pascal, Laboratoire ‘Microorganismes : Génome et Environnement’, BP 10448, 63000 Clermont-Ferrand, France CNRS, UMR 6023, LMGE, 63171 Aubière, France
JULIE AUFAUVRE
Affiliation:
Clermont Université, Université Blaise Pascal, Laboratoire ‘Microorganismes : Génome et Environnement’, BP 10448, 63000 Clermont-Ferrand, France CNRS, UMR 6023, LMGE, 63171 Aubière, France
BRUNO CORBARA
Affiliation:
Clermont Université, Université Blaise Pascal, Laboratoire ‘Microorganismes : Génome et Environnement’, BP 10448, 63000 Clermont-Ferrand, France CNRS, UMR 6023, LMGE, 63171 Aubière, France
FREDERIC DELBAC
Affiliation:
Clermont Université, Université Blaise Pascal, Laboratoire ‘Microorganismes : Génome et Environnement’, BP 10448, 63000 Clermont-Ferrand, France CNRS, UMR 6023, LMGE, 63171 Aubière, France
NICOLAS BLOT*
Affiliation:
Clermont Université, Université Blaise Pascal, Laboratoire ‘Microorganismes : Génome et Environnement’, BP 10448, 63000 Clermont-Ferrand, France CNRS, UMR 6023, LMGE, 63171 Aubière, France
*
*Corresponding author: Université Blaise Pascal, Laboratoire ‘Microorganismes: Génome et Environnement’ CNRS UMR 6023, 63177 Aubière, France. E-mail: nicolas.blot@univ-bpclermont.fr
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Summary

The microsporidian parasite Nosema ceranae is a common pathogen of the Western honeybee (Apis mellifera) whose variable virulence could be related to its genetic polymorphism and/or its polyphenism responding to environmental cues. Since the genotyping of N. ceranae based on unique marker sequences had been unsuccessful, we tested whether a multilocus approach, assessing the diversity of ten genetic markers – encoding nine proteins and the small ribosomal RNA subunit – allowed the discrimination between N. ceranae variants isolated from single A. mellifera individuals in four distant locations. High nucleotide diversity and allele content were observed for all genes. Most importantly, the diversity was mainly present within parasite populations isolated from single honeybee individuals. In contrast the absence of isolate differentiation precluded any taxa discrimination, even through a multilocus approach, but suggested that similar populations of parasites seem to infect honeybees in distant locations. As statistical evolutionary analyses showed that the allele frequency is under selective pressure, we discuss the origin and consequences of N. ceranae heterozygosity in a single host and lack of population divergence in the context of the parasite natural and evolutionary history.

Keywords

Nosema ceranaeparasitehoneybeediversity
Type
Research Article
Information
Parasitology , Volume 140 , Issue 11 , September 2013 , pp. 1346 - 1356
Copyright
Copyright © Cambridge University Press 2013 

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