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Molecular and morphological variation of Paragonimus westermani in Vietnam with records of new second intermediate crab hosts and a new locality in a northern province

Published online by Cambridge University Press:  21 July 2016

N. PHAM DOANH ,
A. LUU TU ,
T. DUNG BUI ,
T. HO LOAN ,
NARIAKI NONAKA ,
YOICHIRO HORII ,
DAVID BLAIR  and
YUKIFUMI NAWA
N. PHAM DOANH*
Affiliation:
Department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam
A. LUU TU
Affiliation:
Vietnam National Lung Hospital, Hanoi, Vietnam
T. DUNG BUI
Affiliation:
Department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam
T. HO LOAN
Affiliation:
Department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam
NARIAKI NONAKA
Affiliation:
Laboratory of Veterinary Parasitic Diseases, Faculty of Agriculture, University of Miyazaki, Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan
YOICHIRO HORII
Affiliation:
Laboratory of Veterinary Parasitic Diseases, Faculty of Agriculture, University of Miyazaki, Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan
DAVID BLAIR
Affiliation:
College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, Australia
YUKIFUMI NAWA
Affiliation:
Research Affairs Office, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
*
*Corresponding author: Department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam. E-mail: pndoanh@yahoo.com
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Summary

Paragonimus westermani is one of the most medically important lung flukes and is widely distributed in Asia. It exhibits considerable variation in morphological, genetic and biological features. In central provinces of Vietnam, a high prevalence of metacercariae of this species has been reported from the crab intermediate host, Vietopotamon aluoiense. In this study, we detected P. westermani metacercariae in two additional crab hosts, Donopotamon haii in Quang Tri Province, central Vietnam and Indochinamon tannanti in Yen Bai Province in the north. The latter is a new locality for P. westermani in a northern region of Vietnam where P. heterotremus is the only species currently known to cause human paragonimiasis. Paragonimus westermani metacercariae found in Vietnam showed considerable morphological variation but slight genetic variation based on DNA sequences from the nuclear ribosomal ITS2 region and the mitochondrial 16S gene. Co-infection of the same individual crabs with P. westermani and P. heterotremus and/or some other Paragonimus species was found frequently, suggesting potential for co-infection in humans. The findings of the present study emphasize the need for highly specific molecular and immunodiagnostic methods to differentially diagnose between P. westermani and P. heterotremus infections.

Keywords

Paragonimus westermanimorphological and molecular variationnew localitynew crab intermediate hosts
Type
Research Article
Information
Parasitology , Volume 143 , Issue 12 , October 2016 , pp. 1639 - 1646
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Copyright
Copyright © Cambridge University Press 2016 

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