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Mimotope-based antigens as potential vaccine candidates in experimental murine cysticercosis

Published online by Cambridge University Press:  14 July 2020

Marianna Nascimento Manhani ,
Cristiane Queixa Tilelli ,
Vanessa da Silva Ribeiro ,
Luiz Ricardo Goulart  and
Julia Maria Costa-Cruz Open the ORCID record for Julia Maria Costa-Cruz [Opens in a new window]
Marianna Nascimento Manhani
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais, 38400-902, Brazil Laboratório de Fisiologia, Universidade Federal de São João del-Rei, R. Sebastião Gonçalves Coelho 400, Divinópolis, Minas Gerais, 35501-296, Brazil
Cristiane Queixa Tilelli
Affiliation:
Laboratório de Fisiologia, Universidade Federal de São João del-Rei, R. Sebastião Gonçalves Coelho 400, Divinópolis, Minas Gerais, 35501-296, Brazil
Vanessa da Silva Ribeiro
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais, 38400-902, Brazil
Luiz Ricardo Goulart
Affiliation:
Laboratório de Nanobiotecnologia, Instituto de Biotecnologia, Universidade Federal de Uberlândia, Av. Amazonas s/n, Bl. 2E, Uberlândia, Minas Gerais, 38400-902, Brazil
Julia Maria Costa-Cruz*
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais, 38400-902, Brazil
*
Author for correspondence: Julia Maria Costa-Cruz, E-mail: costacruz@ufu.br
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Abstract

Human cysticercosis is a public health problem caused by Taenia solium metacestodes; thus, eradication of T. solium transmission by vaccination is an urgent requirement. The Cc48 mimotope from T. solium cysticerci was tested expressed in phage particles (mCc48) and chemically synthesized (sCc48) as a vaccine candidate in experimental murine cysticercosis. For this, BALB/c mice were immunized with mCc48 (G1; n = 40), sCc48 (G2; n = 40) and phosphate-buffered saline (PBS) (G3; n = 40, positive control) and challenged with Taenia crassiceps metacestodes. Another PBS group without parasite challenge was used as a negative control (G4; n = 40). Mice were sacrificed 15, 30, 45 and 60 days post-infection for cysticerci and serum collection. Immunization efficacy was determined by cysticerci counting. Serum samples were tested by ELISA to verify antibody (IgM, IgG, IgA and IgE) and cytokine (IFNγ and IL-4) levels. The sCc48 achieved the highest rates of protection and efficacy (90 and 98%, respectively). The group immunized with mCc48 presented the highest reactivity for IgM, IgG and IgE. All groups presented IL-4, but IFNγ was quite variable among groups. The protection induced by sCc48 synthetic peptide supports further studies of this mimotope as a potential vaccine candidate against cysticercosis.

Keywords

Cysticercosispeptide Cc48Taenia crassicepsvaccine
Type
Research Article
Information
Parasitology , Volume 147 , Issue 12 , October 2020 , pp. 1330 - 1337
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Copyright
Copyright © The Author(s) 2020. Published by Cambridge University Press

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