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Migration of Fasciola hepatica newly excysted juveniles is inhibited by high-mannose and oligomannose-type N-glycan-binding lectins

Published online by Cambridge University Press:  10 July 2017

ANDRES GARCIA-CAMPOS ,
ALAN W. BAIRD  and
GRACE MULCAHY
ANDRES GARCIA-CAMPOS*
Affiliation:
School of Veterinary Medicine, University College Dublin, Veterinary Sciences Centre, Belfield, Dublin 4, Ireland Conway Institute of Biomedical and Biomolecular Research, University College Dublin, Belfield, Dublin 4, Ireland
ALAN W. BAIRD
Affiliation:
School of Veterinary Medicine, University College Dublin, Veterinary Sciences Centre, Belfield, Dublin 4, Ireland Conway Institute of Biomedical and Biomolecular Research, University College Dublin, Belfield, Dublin 4, Ireland
GRACE MULCAHY
Affiliation:
School of Veterinary Medicine, University College Dublin, Veterinary Sciences Centre, Belfield, Dublin 4, Ireland Conway Institute of Biomedical and Biomolecular Research, University College Dublin, Belfield, Dublin 4, Ireland
*
*Corresponding author: School of Veterinary Medicine, University College Dublin, Veterinary Sciences Centre, Belfield, Dublin 4, Ireland. E-mail: andres.garcia-campos@ucd.ie
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Summary

Fasciola hepatica has both zoonotic importance and high economic impact in livestock worldwide. After ingestion by the definitive host, the Newly Excysted Juveniles (NEJ) penetrate the intestine before reaching the peritoneal cavity. The role of some NEJ-derived proteins in invasion has been documented, but the role of NEJ glycans or lectin-binding receptors during initial infection in the gut is still unknown. To address these questions, the migration of NEJ through rat intestine was recorded at 30 min intervals up to 150 min by two ex vivo methods. Firstly, jejunal sheets were challenged with NEJ incubated with biotinylated lectins. Secondly, untreated NEJ were incubated with distal jejunum pre-treated with lectins. Both Concanavalin A (ConA) and Galanthus nivalis (GNL), which recognize mannose-type N-glycans, significantly inhibited NEJ migration across the jejunum. Most of the lectins bound to the tegument and oral sucker of the NEJ, but only ConA and GNL maintained this interaction over 150 min. None of the lectins examined significantly reduced NEJ migration when pre-incubated with jejunal sheets, suggesting that host glycans might not be essential for initial binding/recognition of the gut by NEJ. Agents capable of blocking mannose-type N-glycans on the NEJ tegument may have potential for disrupting infection.

Keywords

Fasciola hepaticaNEJlectinsjejunumConAGNLglycansmannose-type N-glycansmigration inhibition
Type
Research Article
Information
Parasitology , Volume 144 , Issue 13 , November 2017 , pp. 1708 - 1717
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Copyright
Copyright © Cambridge University Press 2017 

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