Hostname: page-component-7479d7b7d-767nl Total loading time: 0 Render date: 2024-07-08T18:26:18.988Z Has data issue: false hasContentIssue false
  • English
  • Français

Membrane transport of aromatic amino acids by Hymenolepis diminuta (Cestoda)

Published online by Cambridge University Press:  06 April 2009

P. W. Pappas  and
H. R. Gamble
P. W. Pappas
Affiliation:
College of Biological Sciences, Department of Zoology, The Ohio State University, Columbus, Ohio 43210
H. R. Gamble
Affiliation:
College of Biological Sciences, Department of Zoology, The Ohio State University, Columbus, Ohio 43210
Get access Rights & Permissions [Opens in a new window]

Summary

Aromatic amino acids (phenylalanine, tryptophan and tyrosine) are absorbed by Hymenolepis diminuta through a combination of mediated (non-Na+-sensitive) transport and diffusion. All 3 amino acids are accumulated against an apparent concentration difference during a 30-min incubation of tapeworms in 0·1 mM 3H-labelled amino acid. Inhibitor studies demonstrate that phenylalanine, tryptophan and tyrosine are mutually competitive inhibitors of the uptake of each other, and the uptake of these amino acids is inhibited by aliphatic amino acids but not by basic or dicarboxylic amino acids. The D- and L-isomers of aromatic amino acids are equally effective in inhibiting aromatic amino acid uptake. The data indicate that at least 3 amino acid transport loci are involved in aromatic amino acid transport by H. diminuta.

Type
Research Article
Information
Parasitology , Volume 81 , Issue 2 , October 1980 , pp. 395 - 403
Copyright
Copyright © Cambridge University Press 1980

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Arme, C. & Coates, A. (1973). Hymenolepis diminuta: active transport of α-aminoisobutyric acid by cisticercoid larvae. International Journal for Parasitology 3, 553–60.Google Scholar
Crane, R. K. (1977). The gradient hypothesis and other models of carrier-mediated active transport. Reviews of Physiology, Biochemistry and Pharmacology 78, 99–159.Google Scholar
Harris, B. G. & Read, C. P. (1968). Studies on membrane transport. III. Further characterization of amino acid systems in Hymenolepis diminuta (Cestoda). Comparative Biochemistry and Physiology 26, 545–52.Google Scholar
Hofstee, B. H. J. (1956). Graphic analysis of single enzyme systems. Enzymologia 17, 273–8.Google Scholar
Kilejian, A. (1966). Permeation of L-proline in the cestode, Hymenolepis diminuta. Journal of Parasitology 52, 1108–15.CrossRefGoogle ScholarPubMed
Laws, G. F. & Read, C. P. (1969). Effect of the amino carboxy group on amino acid transport in Hymenolepis diminuta (Cestoda). Comparative Biochemistry and Physiology 30, 129–32.CrossRefGoogle Scholar
Mettrick, D. F. & Podesta, R. B. (1974). Ecological and physiological aspects of helrninthhost interactions in the mammalian gastrointestinal canal. Advances in Parasitology 12, 183–278.CrossRefGoogle ScholarPubMed
Pappas, P. W. & Freeman, B. A. (1975). Sodium dependent glucose transport in the mouse bile duct tapeworm, Hymenolepis microstoma. Journal of Parasitology 61, 434–9.Google Scholar
Pappas, P. W. & Read, C. P. (1972). Sodium and glucose fluxes across the brush border of a flatworm (Calliobothrium verticillatum, Cestoda). Journal of Comparative Physiology 81, 215–28.CrossRefGoogle Scholar
Pappas, P. W. & Read, C. P. (1973). Permeability and membrane transport in the larva of Taenia crassiceps. Parasitology 66, 33–42.CrossRefGoogle ScholarPubMed
Pappas, P. W. & Read, C. P. (1975). Membrane transport in helminth parasites: a review. Experimental Parasitology 37, 469–530.Google Scholar
Pappas, P. W., Uglem, G. L. & Read, C. P. (1973 a). Taenia crassiceps: absorption of hexoses and partial characterization of Na+-dependent glucose absorption by larvae. Experimental Parasitology 33, 127–37.Google Scholar
Pappas, P. W., Uglem, G. L. & Read, C. P. (1973 b). Mechanisms and specificity of amino acid transport in Taenia crassiceps larvae (Cestoda). International Journal for Parasitology 3, 641–51.Google Scholar
Pappas, P. W., Uglem, G. L. & Read, C. P. (1974). Anion and cation requirements for glucose and methionine accumulation in Hymenolepis diminuta (Cestoda). Biological Bulletin 146, 56–66.Google Scholar
Read, C. P., Rothman, A. H. & Simmons, J. E. Jr (1963). Studies on membrane transport, with special reference to parasite–host integration. Annals of the New York Academy of sciences 113, 154–205.Google Scholar
Read, C. P., Stewart, G. L. & Pappas, P. W. (1974). Glucose and sodium fluxes across the brush border of Hymenolepis diminuta (Cestoda). Biological Bulletin 147, 146–62.Google Scholar
Schultz, S. G. & Curran, P. F. (1970). Coupled transport of sodium and organic solutes. Physiological Reviews 50, 637–718.Google Scholar
Senturia, J. B. (1964). Studies on the absorption of methionine by the cestode, Hymenolepis citelli. Comparative Biochemistry and Physiology 12, 259–72.Google Scholar
Uglem, G. L. (1976). Evidence for a sodium ion exchange carrier linked with glucose transport across the brush border of a flatworm (Hymenolepis diminuta, Cestoda). Biochimica et biophysica acta 443, 126–36.Google Scholar