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Inventory and analysis of ATP-binding cassette (ABC) systems in Brugia malayi

Published online by Cambridge University Press:  17 March 2010

B. F. ARDELLI ,
L. E. STITT  and
J. B. TOMPKINS
B. F. ARDELLI*
Affiliation:
Department of Biology, Brodie Science Building, Brandon University, 270-18th Street, Brandon, Manitoba, CanadaR7A 6A9
L. E. STITT
Affiliation:
Department of Biology, Brodie Science Building, Brandon University, 270-18th Street, Brandon, Manitoba, CanadaR7A 6A9
J. B. TOMPKINS
Affiliation:
Department of Biology, Brodie Science Building, Brandon University, 270-18th Street, Brandon, Manitoba, CanadaR7A 6A9
*
*Corresponding author: Tel: +1 204 571 8563. Fax: +1 204 728 7346. E-mail: ardellib@brandonu.ca
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Summary

ABC systems are one of the largest described protein superfamilies. These systems have a domain organization that may contain 1 or more transmembrane domains (ABC_TM1F) and 1 or 2 ATP-binding domains (ABC_2). The functions (e.g., import, export and DNA repair) of these proteins distinguish the 3 classes of ABC systems. Mining and PCR-based cloning were used to identify 33 putative ABC systems from the Brugia malayi genome. There were 31 class 2 genes, commonly called ABC transporters, and 2 class 3 genes. The ABC transporters were divided into subfamilies. Three belonged to subfamily A, 16 to subfamily B, 5 to subfamily C, 1 to subfamily E and 3 to subfamilies F and G, respectively. None were placed in subfamilies D and H. Similar to other ABC systems, the ABC_2 domain of B. malayi genes was conserved and contained the Walker A and B motifs, the signature sequence/linker region and the switch region with the conserved histidine. The ABC_TM1F domain was less conserved. The relative abundance of ABC systems was quantified using real-time reverse transcription PCR and was significantly higher in female adults of B. malayi than in males and microfilaria, particularly those in subfamilies B and C, which are associated with drug resistance.

Keywords

Brugia malayiABC systemdomainphylogeneticconstitutiveexpressionnematode
Type
Research Article
Information
Parasitology , Volume 137 , Issue 8 , July 2010 , pp. 1195 - 1212
Copyright
Copyright © Cambridge University Press 2010

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