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Immunity to Schistosoma mansoni in congenitally athymic, irradiated and mast cell-depleted rats

Published online by Cambridge University Press:  06 April 2009

M. J. Ford ,
Q. D. Bickle  and
M. G. Taylor
M. J. Ford
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
Q. D. Bickle
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
M. G. Taylor
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
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Summary

Immunity to Schistosoma mansoni was investigated in congenitally athymic (Nu/Nu) rats, irradiated rats and in mast cell-depleted rats. Nu/Nu rats failed to develop significant resistance following vaccination with irradiated cercariae, although Nu/Nu recipients of serum from vaccinated Fischer rats (VRS) manifested resistance comparable to heterozygous controls, suggesting that T-cells were required in the induction of resistance but were not involved in the efferent arm of antibody-dependent elimination. Radiosensitive cells (including eosinophils, basophils, neutrophils, lymphocytes and mast cells) were apparently not essential for the antibody-dependent elimination of lung or post-lung stages since irradiated (700–750 rad.) recipients of VRS manifested comparable degrees of resistance to unirradiated controls in spite of a greater than 85% reduction in total blood leucocyte counts after irradiation. Depletion of 99 % of tissue mast cells by treatment of rats with Compound 48/80 had no significant effect on the attrition of a challenge infection in rats rendered immune by vaccination with irradiated cercariae or by transfer of VRS. However, there was a significant increase in worm recovery in unimmunized and mast cell-depleted or irradiated rats, indicating that mast cells and perhaps other radio-isotope sensitive cells may be involved in innate resistance.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 2 , April 1987 , pp. 313 - 326
Copyright
Copyright © Cambridge University Press 1987

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