Hostname: page-component-77c89778f8-m8s7h Total loading time: 0 Render date: 2024-07-16T09:49:21.571Z Has data issue: false hasContentIssue false
  • English
  • Français

IgG response of rats and humans to the released products of schistosomula of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

Claudie Verwaerde ,
C. Auriault ,
Martine Damonneville ,
J.-M. Grzych ,
R. Pierce  and
A. Capron
Claudie Verwaerde
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille cedex, France
C. Auriault
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille cedex, France
Martine Damonneville
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille cedex, France
J.-M. Grzych
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille cedex, France
R. Pierce
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille cedex, France
A. Capron
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille cedex, France
Get access Rights & Permissions [Opens in a new window]

Extract

The participation of products released from Schistosoma mansoni schistosomula (SRP-A) in the IgG antibody response of infected Brown-Norway rats and infected humans has been studied using immunoprecipitation with various antigenic preparations and in in vitro cytotoxicity assays. A large number of SRP-A molecules with a wide range of molecular weights was recognized by infected rat and human sera. Anti-SRP-A antibodies appeared in rat sera from day 28 after infection. In infected humans, a variable pattern of SRP-A recognition was observed between individuals. IgG antibodies obtained by immunization of rats with SRP-A without addition of adjuvants reacted with 3 major schistosomula surface proteins with molecular weights of 38, 32 and 21 kDa. These latter molecules were also revealed strongly by infected rat sera. Moreover, these antibodies were able to kill schistosomula in vitro in the presence of complement or eosinophils.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 3 , June 1985 , pp. 509 - 518
Copyright
Copyright © Cambridge University Press 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Auriault, C., Damonneville, M., Verwaerde, C., Pierce, R. J., Joseph, M., Capron, M. & Capron, A. (1984). Rat lgE directed against schistosomula released products (SRP-A) is cytotoxic for Schistosoma mansoni schistosomula. European Journal of Immunology 14, 132–8.CrossRefGoogle Scholar
Bazin, H., Capron, A., Capron, M., Joseph, M., Dessaint, J. P. & Pauwels, R. (1980). Effect of neonatal injection of anti-μ antibodies on immunity to schistosomes (S. mansoni) in the rat. Journal of Immunology 124, 2373–7.CrossRefGoogle ScholarPubMed
Butterworth, A. E., Sturrock, R. F., Houba, C. & Rees, P. M. (1975). Eosinophils as mediators of antibody-dependent damage to schistosomula. Nature, London 256, 727–9.CrossRefGoogle ScholarPubMed
Capron, A., Capron, M., Dupas, H., Bout, D. & Petitprez, A. (1974). Etude in vitro des phénomènes immunologiques dans la schistosomiase humaine et expérimentale. I. Etude comparative in vitro de l'activité léthale d'immunsérums sur les formes immatures et sur les adults de Schistosoma mansoni. Parasitology 4, 613–23.Google Scholar
Capron, A., Dessaint, J. P., Capron, M. & Bazin, H. (1975). Specific IgE antibodies in immune adherence of normal macrophages to Schistosoma mansoni schistosomula. Nature, London 253, 474–5.CrossRefGoogle Scholar
Capron, A., Dessaint, J. P., Capron, M., Joseph, M. & Torpier, G. (1982). Effector mechanisms of immunity to schistosomes and their regulation. Immunological Reviews 61, 41–66.CrossRefGoogle ScholarPubMed
Capron, M., Bazin, H., Joseph, M. & Capron, A. (1981). Evidence for IgG-dependent cytotoxicity by rat eosinophils. Journal of Immunology 126, 1764–8.CrossRefGoogle ScholarPubMed
Capron, M., Capron, A., Torpier, G., Bazin, H., Bout, D. & Joseph, M. (1978). Eosinophil-dependent cytotoxicity in rat schistosomiasis. Involvement of IgG2a antibodies and role of mast cells. European Journal of Immunology 8, 127–33.CrossRefGoogle ScholarPubMed
Capron, M., Nogueira-Queiroz, J. A., Papin, J. P. & Capron, A. (1984). Interactions between eosinophils and antibodies: in vivo protective role against rat schistosomiasis. Cellular Immunology 83, 60–72.CrossRefGoogle ScholarPubMed
Clegg, J. A. & Smithers, S. R. (1972). The effects of immune Rhesus monkey serum on schistosomula of S. mansoni during cultivation in vitro. International Journal for Parasitology 2, 79–98.CrossRefGoogle Scholar
Dean, D. A. (1977). Decreased binding of cytotoxic antibody by developing schistosomula: evidence for a surface change independent of host-antigen absorption and membrane turnover. Journal of Parasitology 63, 418–26.CrossRefGoogle Scholar
Dissous, C., Dissous, C. & Capron, A. (1981). Isolation and characterization of surface antigens from Schistosoma mansoni schistosomula. Molecular and Biochemical Parasitology 3, 215–25.CrossRefGoogle ScholarPubMed
Dissous, C., Grzych, J. M. & Capron, A. (1982). Schistosoma mansoni surface antigen defined by a rat monoclonal IgG2a. Journal of Immunology 129, 2232–4.CrossRefGoogle ScholarPubMed
Greenwood, F. C., Hunter, W. & Bolver, J. S. (1963). The preparation of 1–131 labelled human growth hormone of high specific radioactivity. The Biochemical Journal 89, 114–23.CrossRefGoogle Scholar
Grzych, J. M., Capron, M., Bazin, H. & Capron, A. (1982). In vitro and in vivo effector function of rat IgG2a monoclonal anti-S. mansoni antibodies, Journal of Immunology 129, 2739–43.CrossRefGoogle ScholarPubMed
Joseph, M., Auriault, C., Capron, A., Vorng, H. & Viens, P. (1983). A new function for platelets: IgE-dependent killing of Schistosomes. Nature, London 303, 810–12.CrossRefGoogle ScholarPubMed
Knight, M., Simpson, A. J. G., Payares, G., Chaudri, M. & Smithers, S. R. (1984). Cell-free synthesis of Schistosoma mansoni surface antigens: stage specificity of their expression. The EMBO Journal 3, 213–19.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Markwell, M. A. K. & Fox, C. F. (1978). Surface specific iodination of membrane proteins of viruses and eukaryotic cells using 1, 3, 4, 6-tetrachloro-3α-6α-diphenylglycosuril. Biochemistry 17, 4807–17.CrossRefGoogle Scholar
Ramalho-Pinto, G., Gazzinelli, G., Howells, R. E., Mota-Santos, T. A., Figueiredo, E. A. & Pellegrino, J. (1974). Schistosoma mansoni: defined system for stepwise transformation of cercariae to schistosomula in vitro. Experimental Parasitology 36, 360–72.CrossRefGoogle Scholar
Samuelson, J. C. & Caulfield, J. P. (1982). Loss of Covalently labelled glycoproteins and glycolipids from the surface of newly transformed schistosomula of Schistosoma mansoni. The Journal of Cell Biology 94, 363–9.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of S. mansoni and the recovery of the adult worms. Parasitology 55, 695–700.CrossRefGoogle Scholar
Smithers, S. R., Terry, R. J. & Hockley, D. J. (1969). Host antigens in schistosomiasis. Proceedings of the Royal Society 171, 483–94.Google ScholarPubMed