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Histopathologic evaluation of experimental murine neurocysticercosis after treatment with albendazole/nitazoxanide combination

Published online by Cambridge University Press:  01 April 2020

Guilherme A. Sampaio Open the ORCID record for Guilherme A. Sampaio [Opens in a new window] ,
Larissa V. Zago Open the ORCID record for Larissa V. Zago [Opens in a new window] ,
Nayana F. de Lima Open the ORCID record for Nayana F. de Lima [Opens in a new window] ,
Guaraciara de A. Picanço Open the ORCID record for Guaraciara de A. Picanço [Opens in a new window] ,
Ruy de S. Lino Júnior Open the ORCID record for Ruy de S. Lino Júnior [Opens in a new window]  and
Marina C. Vinaud Open the ORCID record for Marina C. Vinaud [Opens in a new window]
Guilherme A. Sampaio
Affiliation:
Medicine School, Federal University of Goias, Goiânia, CEP 74605-050, Goiás, Brazil
Larissa V. Zago
Affiliation:
Medicine School, Federal University of Goias, Goiânia, CEP 74605-050, Goiás, Brazil
Nayana F. de Lima
Affiliation:
Tropical Pathology and Public Health Institute, Federal University of Goias, Goiânia, CEP 74605-050, Goiás, Brazil
Guaraciara de A. Picanço
Affiliation:
Tropical Pathology and Public Health Institute, Federal University of Goias, Goiânia, CEP 74605-050, Goiás, Brazil
Ruy de S. Lino Júnior
Affiliation:
Tropical Pathology and Public Health Institute, Federal University of Goias, Goiânia, CEP 74605-050, Goiás, Brazil
Marina C. Vinaud*
Affiliation:
Tropical Pathology and Public Health Institute, Federal University of Goias, Goiânia, CEP 74605-050, Goiás, Brazil
*
Author for correspondence: Marina Clare Vinaud, E-mail: mvinaud@ufg.br
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Abstract

Neurocysticercosis (NCC) is the most common helminthic brain infection related to epilepsy. Only albendazole (ABZ) and praziquantel are used in its treatment. The development of new therapeutics has been encouraged. Taenia crassiceps cysticerci intracranial infection is the experimental model used in NCC studies. This study evaluated the histopathology of the brains of BALB/c mice experimentally infected with T. crassiceps cysticerci after the treatment with the ABZ/nitazoxanide (NTZ) combination. Thirty days after the inoculation the mice received an oral single dose of the ABZ/NTZ combination (40 mg kg−1 each). The control groups were treated with: NaCl 0.9%; ABZ or NTZ. The histopathologic evaluation of the brains was performed 24 h after treatment. The ABZ treatment induced discrete mononuclear inflammatory infiltration, meningitis, gliosis, hyperaemia and hippocampus compression; moderate ependimitis and oedema. The NTZ treatment induced accentuated inflammatory infiltration, foamy macrophages, ependimitis, choroiditis, gliosis and hyperaemia and moderate oedema. The ABZ/NTZ combination treatment induced a significant decrease in the polymorphonuclear inflammatory infiltration, ependimitis, choroiditis, gliosis, hyperaemia and ventriculomegaly in comparison with the other groups. The cysticerci showed destruction of the tegument not observed in other groups. The ABZ/NTZ combination is efficient as the parasite showed signs of destruction and lower damage to the host's tissue.

Keywords

Albendazoleanthelminthic drugsexperimental neurocysticercosisnitazoxanidepathologic evaluationTaenia crassiceps
Type
Research Article
Information
Parasitology , Volume 147 , Issue 7 , June 2020 , pp. 822 - 827
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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References

Baranwal, AK, Singhi, PD, Khandelwal, N and Singhi, SC (1998) Albendazole therapy in children with focal seizures and single small enhancing computerized tomographic lesions: a randomized, placebo-controlled, double blind trial. The Pediatric Infectious Disease Journal 17, 696–700.CrossRefGoogle ScholarPubMed
Cangalaya, C, Bustos, JA, Calcina, J, Vargas-Calla, A, Suarez, D, Gonzalez, AE, Chacaltana, J, Guerra-Giraldez, C, Mahanty, S, Nash, TE and García, HH and Cysticercosis Working Group in Peru (2016) Perilesional inflammation in neurocysticercosis – relationship between contrast-enhanced magnetic resonance imaging, evans blue staining and histopathology in the pig model. PLoS Neglected Tropical Diseases 10, e0004869.CrossRefGoogle ScholarPubMed
Cangalaya, C, Bustos, JA, Calcina, J, Vargas-Calla, A, Mamani, J, Suarez, D, Arroyo, G, Gonzalez, AE, Chacaltana, J, Guerra-Giraldez, C, Mahanty, S, Nash, TE and García, HH and Cysticercosis Working Group in Peru (2017) Radiological evolution of porcine neurocysticercosis after combined antiparasitic treatment with praziquantel and albendazole. PLoS Neglected Tropical Diseases 11, e0005624.CrossRefGoogle ScholarPubMed
Cardona, AR, Restrepo, BI, Jaramillo, JM and Teale, JM (1999) Development of an animal model for neurocysticercosis: immune response in the central nervous system is characterized by a predominance of γδ T cells. The Journal of Immunology 162, 995–1002.Google Scholar
Carpio, A, Fleury, A and Hauser, WA (2013) Neurocysticercosis five new things. Neurology Clinical Practice 3, 118–125.CrossRefGoogle ScholarPubMed
Fleury, A, Cardenas, G, Adalid-Peralta, L, Fragoso, G and Sciutto, E (2016) Immunopathology in Taenia solium neurocysticercosis. Parasite Immunology 38, 147–157.CrossRefGoogle ScholarPubMed
Fogang, YF, Savadogo, AA, Camara, M, Toffa, DH, Basse, A, Sow, AD and Ndiaye, MM (2015) Managing neurocysticercosis: challenges and solutions. International Journal of General Medicine 8, 333–344.CrossRefGoogle ScholarPubMed
Fong, GC and Cheung, RT (1997) Caution with praziquantel in neurocysticercosis. Stroke 28, 1648–1649.Google ScholarPubMed
Garcia, HH and Del Brutto, OH (2017) Antiparasitic treatment of neurocysticercosis – The effect of cyst destruction in seizure evolution. Epilepsy & Behavior 76, 158–162.CrossRefGoogle ScholarPubMed
Gogia, S, Talukdar, B, Choudhury, V and Arora, BS (2003) Neurocysticercosis in children: clinical findings and response to albendazole therapy in a randomized, double-blind, placebo-controlled trial in newly diagnosed cases. Transactions of the Royal Society of Tropical Medicine and Hygiene 97, 416–421.CrossRefGoogle Scholar
Gonzales, I, Rivera, JT and Garcia, HH (2016) Pathogenesis of Taenia solium taeniasis and cysticercosis. Parasite Immunology 38, 136–146.CrossRefGoogle ScholarPubMed
Kalra, V, Dua, T and Kumar, V (2003) Efficacy of albendazole and short-course dexamethasone treatment in children with 1 or 2 ring-enhancing lesions of neurocysticercosis: a randomized controlled trial. The Journal of Pediatrics 143, 111–114.CrossRefGoogle ScholarPubMed
Lalchhandama, K (2010) In vitro effects of albendazole on Raillietina echinobothrida, the cestode of chicken, Gallus domesticus. Journal of Young Pharmacists 2, 374–378.CrossRefGoogle ScholarPubMed
Lima, NF, Picanço, GA, Alves, DSMM, Silva, LD, Isac, E, Costa, TL, Lino Junior, RS and Vinaud, MC (2019) Oral nitazoxanide treatment of experimental neurocysticercosis induces gluconeogenesis in Taenia crassiceps cysticerci. Acta Tropica 190, 361–364.CrossRefGoogle ScholarPubMed
Lino Junior, RS, Ribeiro, PM, Antonelli, EJ, Faleiros, AC, Terra, SA, dos Reis, MA and Teixeira, VP (2002) Características evolutivas do Cysticercus cellulosae no encéfalo e no coração. Revista da Sociedade Brasileira de Medicina Tropical 35, 617–622.CrossRefGoogle Scholar
Mahanty, S, Madrid, EM and Nash, TE (2013) Quantitative screening for anticestode drugs based on changes in baseline enzyme secretion by Taenia crassiceps. Antimicrobial Agents and Chemotherapy 57, 990–995.CrossRefGoogle ScholarPubMed
Markoski, MM, Trindade, ES, Cabrera, G, Laschuk, A, Galanti, N, Zaha, A, Nader, HB and Ferreira, HB (2006) Praziquantel and albendazole damaging action on in vitro developing Mesocestoides corti (Platyhelminthes: Cestoda). Parasitology International 55, 51–61.CrossRefGoogle Scholar
Matos-Silva, H, Reciputti, BP, de Paula, EC, Oliveira, AL, Moura, VBL, Vinaud, MC, Oliveira, MAP and Lino-Junior, RS (2012) Experimental encephalitis caused by Taenia crassiceps cysticerci in mice. Arquivos de Neuro-Psiquiatria 70, 287–292.CrossRefGoogle ScholarPubMed
Moura, VBL, Lima, SB, Matos-Silva, H, Vinaud, MC, Loyola, PRAN and Lino, RS (2016) Cellular immune response in intraventricular experimental neurocysticercosis. Parasitology 143, 334–342.CrossRefGoogle ScholarPubMed
Palomares-Alonso, F, Piliado, JC, Palencia, G, Ortiz-Plata, A and Jung-Cook, H (2007) Efficacy of nitazoxanide, tizoxanide and tizoxanide/albendazole sulphoxide combination against Taenia crassiceps cysts. Journal of Antimicrobial Chemotherapy 59, 212–218.CrossRefGoogle ScholarPubMed
Palomares, F, Palencia, G, Ambrosio, JR, Ortiz, A and Jung-Cook, H (2006) Evaluation of the efficacy of albendazole sulphoxide and praziquantel in combination on Taenia crassiceps cysts: in vitro studies. Journal of Antimicrobial Chemotherapy 57, 482–488.CrossRefGoogle ScholarPubMed
Reddy, DS and Volkmer, R II (2017) Neurocysticercosis as an infectious acquired epilepsy worldwide. Seizure 52, 176–181.CrossRefGoogle ScholarPubMed
Shakya, A, Bhat, HR and Ghosh, SK (2018) Update on nitazoxanide: a multifunctional chemotherapeutic agent. Current Drug Discovery Technologies 15, 201–213.CrossRefGoogle ScholarPubMed
Shou, J, Kong, X, Wang, X, Tang, Y, Wang, C, Wang, M, Zhang, L, Liu, Y, Fei, C, Xue, F, Li, J and Zhang, K (2019) Tizoxanide inhibits inflammation in LPS-activated RAW264.7 macrophages via the suppression of NF-κB and MAPK activation. Inflammation 42, 1336–1349.CrossRefGoogle ScholarPubMed
Singh, SK, Prasad, KN, Singh, AK, Gupta, KK, Singh, A, Tripathi, M and Gupta, RK (2017) Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis. Experimental Parasitology 182, 1–8.CrossRefGoogle ScholarPubMed
Singhi, P, Jain, V and Khandelwal, N (2004) Corticosteroids versus albendazole for treatment of single small enhancing computed tomographic lesions in children with neurocysticercosis. Journal of Child Neurology 19, 323–327.CrossRefGoogle ScholarPubMed
Vaz, AJ, Nunes, CM, Piazza, RM, Livramento, JA, Da Silva, MV and Nakamura, PM (1997) Immunoblot with cerebrospinal fluid from patients with neurocysticercosis using antigen from cysticerci of Taenia solium and Taenia crassiceps. The American Journal of Tropical Medicine and Hygiene 57, 354–357.CrossRefGoogle ScholarPubMed
Vinaud, MC, de Lino, RS Jr and Bezerra, JC (2007) Taenia crassiceps organic acids detected in cysticerci. Experimental Parasitology 116, 335–339.CrossRefGoogle ScholarPubMed
White, AC Jr, Coyle, CM, Rajshekhar, V, Singh, G, Hauser, WA, Mohanty, A, Garcia, HH and Nash, TE (2018) Diagnosis and treatment of neurocysticercosis: 2017 clinical practice guidelines by the Infectious Diseases Society of America (IDSA) and the American Society of Tropical Medicine and Hygiene (ASTMH). The American Journal of Tropical Medicine and Hygiene 98, 945–966CrossRefGoogle Scholar
WORLD HEALTH ORGANIZATION (2014) Assembling a framework for intensified control of taeniasis and neurocysticercosis caused by Taenia solium: Report of an informal consultation. Genebra: WHO Press, 32p.Google Scholar