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Haemogregarines of freshwater turtles from Southeast Asia with a description of Haemogregarina sacaliae sp. n. and a redescription of Haemogregarina pellegrini Laveran and Pettit, 1910

Published online by Cambridge University Press:  04 March 2015

NELA DVOŘÁKOVÁ ,
JANA KVIČEROVÁ ,
MARTIN HOSTOVSKÝ  and
PAVEL ŠIROKÝ
NELA DVOŘÁKOVÁ
Affiliation:
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic CEITEC-Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic
JANA KVIČEROVÁ
Affiliation:
Biology Centre, Academy of Sciences of the Czech Republic, Institute of Parasitology, Branišovská 31, 370 05 České Budějovice, Czech Republic Department of Parasitology, Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05 České Budějovice, Czech Republic
MARTIN HOSTOVSKÝ
Affiliation:
Department of Biochemistry, Biophysics and Chemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic
PAVEL ŠIROKÝ*
Affiliation:
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic CEITEC-Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic
*
*Corresponding author: Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic. E-mail: sirokyp@vfu.cz
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Summary

The uniform morphology of the developmental stages of Haemogregarina species and the insufficient information supplied by the simplistic descriptions of previous authors complicates their differential diagnosis and proper species identification. In this study, we detected Haemogregarina spp. in 6 out of 22 (27·2%) examined turtles originating from Southeast Asia, Malayemys subtrijuga (n = 4), Sacalia quadriocellata (n = 1) and Platysternon megacephalum (n = 1), and compared them with the available literature data. Microscopic analysis of our isolates distinguished 2 morphological species, Haemogregarina pellegrini and one new species, being described in this paper as Haemogregarina sacaliae sp. n. Phylogenetic analyses based on 1210 bp long fragment of 18S rDNA sequences placed both haemogregarines firmly within the monophyletic Haemogregarina clade. Isolates of H. pellegrini from 2 distantly related turtle hosts, M. subtrijuga and P. megacephalum, were genetically identical. Despite the fact that numerous Haemogregarina species of turtles have been described, the incompleteness of the morphological data and relatively low host specificity provides the space for large synonymy within this taxon. Therefore, a complex approach combining microscopic analyses together with molecular-genetic methods should represent the basic standard for all taxonomic studies.

Keywords

HaemogregarinesHaemogregarinaSacaliaPlatysternonMalayemysphylogeny18S rDNA
Type
Research Article
Information
Parasitology , Volume 142 , Issue 6 , May 2015 , pp. 816 - 826
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Copyright
Copyright © Cambridge University Press 2015 

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