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Genetic characterization of members of the genus Contracaecum (Nematoda: Anisakidae) from fish-eating birds from west-central Florida, USA, with evidence of new species

Published online by Cambridge University Press:  13 March 2007

S. D'AMELIO ,
N. B. BARROS ,
S. INGROSSO ,
D. A. FAUQUIER ,
R. RUSSO  and
L. PAGGI
S. D'AMELIO*
Affiliation:
Department of Public Health Science, University of Rome “La Sapienza”, P. le Aldo Moro, 5, 00185 Rome, Italy
N. B. BARROS
Affiliation:
Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, FL34236, USA
S. INGROSSO
Affiliation:
Department of Public Health Science, University of Rome “La Sapienza”, P. le Aldo Moro, 5, 00185 Rome, Italy
D. A. FAUQUIER
Affiliation:
Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, FL34236, USA
R. RUSSO
Affiliation:
Department of Pathology, University of California San Diego, La Jolla, CA 92093, USA
L. PAGGI
Affiliation:
Department of Public Health Science, University of Rome “La Sapienza”, P. le Aldo Moro, 5, 00185 Rome, Italy
*
*Corresponding author: Department of Public Health Science, University of Rome “La Sapienza”, P. le Aldo Moro, 5, 00185, Rome, Italy. Tel: +39 0649914671. Fax: +39 0649914644. E-mail: stefano.damelio@uniroma1.it
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Summary

Specimens of Contracaecum spp. from Phalacrocorax auritus and Pelecanus occidentalis from Florida were characterized by sequencing of the small subunit of the mitochondrial ribosomal RNA gene (rrnS) and by PCR-based RFLP analysis of the same gene and of the internal transcribed spacers (ITS) of nuclear ribosomal DNA. Analyses of the rrnS sequence data using the MP and UPGMA approaches yielded trees with similar topologies, delineating 3 main clusters. Specimens from Ph. auritus, morphologically assigned to C. rudolphii (s.l.), were part of the cluster comprising also the other 2 species of the C. rudolphii complex (A and B), but representing a genetically distinct group, potentially corresponding to a distinct lineage within the complex, provisionally named as C. rudolphii C. The second cluster comprised 5 individuals from P. occidentalis, which formed a genetically relatively homogeneous group. The rrnS data indicate that these specimens (indicated as Contracaecum sp. 1) are clearly genetically different from the morphologically most closely related species, i.e. C. rudolphii (s.l.) and C. microcephalum, and could represent a new species. The third cluster comprised 7 specimens from P. occidentalis morphologically assigned to C. multipapillatum (s.l.). These were shown to be genetically homogeneous and related to but quite distinct from C. multipapillatum from Greece, although additional studies are needed to assess their status. PCR-RFLP based markers for the quick identification of these taxa are provided.

Keywords

ContracaecumPhalacrocorax auritusPelecanus occidentalisnuclear and mitochondrial ribosomal DNAFloridaUSA
Type
Research Article
Information
Parasitology , Volume 134 , Issue 7 , July 2007 , pp. 1041 - 1051
Copyright
Copyright © Cambridge University Press 2007

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