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Features of amino acid metabolism in Moniliformis moniliformis (Acanthocephala) in vitro

Published online by Cambridge University Press:  06 April 2009

P. F. V. Ward  and
D. W. T. Crompton
P. F. V. Ward
Affiliation:
Department of Parasitology, The Molteno Institute, University of Cambridge, Downing Street, Cambridge CB2 3EE
D. W. T. Crompton*
Affiliation:
Department of Zoology, University of Glasgow, Glasgow G12 8QQ
*
*Reprint requests: Professor D. W. T. Crompton, Department of Zoology, University of Glasgow, Glasgow G12 8QQ.
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Extract

Experiments to investigate the metabolism of glycine, L-glutamic acid and L-aspartic acid by Moniliformis moniliformis were carried out by incubating adult worms aerobically for 3 h at 37°C in Tyrode's solution containing either [U-14C]glycine, L-[U-14C]glutamic acid, L-[U-14C]aspartic acid or L-[4-14C]aspartic acid. Much of the glycine and glutamic acid was absorbed by the worms, but little of either was metabolized. Aspartic acid was readily taken up and metabolized. After incubating with L[U-14C]aspartic acid, most radioactivity was found in ethanol and a volatile compound, presumed to be carbon dioxide, with smaller amounts in lactate, alanine, acetate, malate, glucose and succinate. After incubating with L-[4-14C]aspartic acid, most radioactivity was found in lactate and the presumed CO2 with small amounts in alanine, malate and succinate. No radioactivity was found associated with ethanol or acetate. Possible metabolic pathways and suggestions for a relationship between the metabolism of aspartate with that of alanine and serine in this parasite are discussed.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 3 , June 1987 , pp. 533 - 541
Copyright
Copyright © Cambridge University Press 1987

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References

Bergmeyer, H. U. & Bernt, E. (1974). Methods of Enzymatic Analysis (ed. Bergmeyer, H. U.), pp. 727 and 752. Weinheim: Verlag Chemie; New York and London: Academic Press.Google Scholar
Crompton, D. W. T. & Walters, D. E. (1972). An analysis of the course of infection of Moniliformis dubius in rats. Parasitology 64, 517–23.Google Scholar
Crompton, D. W. T. & Ward, P. F. V. (1984). Selective metabolism of L-serine by Moniliformis (Acanthocephala) in vitro. Parasitology 89, 133–44.CrossRefGoogle ScholarPubMed
Hamilton, P. B. (1963). Ion exchange chromatography of amino acids. Analytical Chemistry 35, 2055–64.CrossRefGoogle Scholar
Harlow, G. A. & Morman, D. H. (1964). Automatic ion exclusion-partition chromatography of acids. Analytical Chemistry 36, 2438–42.CrossRefGoogle Scholar
Huskisson, N. S. & Ward, P. F. V. (1980). Solving the problems of counting 14C02 produced in biological experiments. In Liquid Scintillation Counting: Recent Applications and Development (ed. Peng, C. T., Horrocks, D. L. and Alpen, E. L.), pp. 111–32. New York and London: Academic Press.Google Scholar
Lackie, J. M. (1972). The course of infection and growth of Moniliformis dubius (Acanthocephala) in the intermediate host Periplaneta americana. Parasitology 64, 95–106.CrossRefGoogle ScholarPubMed
Lanoue, K., Nicklas, W. J. & Williamson, J. R. (1970). Control of citric acid cycle activity in rat heart mitochondria. Journal of Biological Chemistry 245, 102–11.CrossRefGoogle ScholarPubMed
Nations, C., Hicks, T. C. & Ubelaker, J. C. (1973). CO2 production by extracts of Hymenolepis diminuta (Cestoda: Hymenolepididae) with aspartate and α-ketoglutarate as substrates. Journal of Parasitology 59, 112–16.Google Scholar
Nesheim, M. C., Crompton, D. W. T., Arnold, S. & Barnard, D. (1977). Dietary relations between Moniliformis (Acanthocephala) and laboratory rats. Proceedings of the Royal Society of London, B 197, 363–83.Google Scholar
Tanaka, R. D. & Macinnis, A. J. (1980). Analyses of the pseudocoelomic fluid from Moniliformis dubius. Journal of Parasitology 66, 354–5.Google Scholar
Ward, P. F. V. (1982). Aspects of helminth metabolism. Parasitology 84, 177–94.CrossRefGoogle ScholarPubMed
Ward, P. F. V. & Crompton, D. W. T. (1986). Linked metabolism of L-serine and L-alanine by Moniliformis moniliformis (Acanthocephala) in vitro. Parasitology 93, 333–40.CrossRefGoogle ScholarPubMed
Ward, P. F. V. & Huskisson, N. S. (1978). The energy metabolism of adultHaemonchus contortus in vitro. Parasitology 77, 255–71.Google Scholar
Wheaton, R. M. & Bauman, W. C. (1953). Non-ionic separations with ion exchange resins. Annals of the New York Academy of Science 57, 159–76.CrossRefGoogle Scholar