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Expression of circumsporozoite proteins revealed in situ in the mosquito stages of Plasmodium berghei by the Lowicryl-immunogold technique

Published online by Cambridge University Press:  06 April 2009

A. J. Hamilton ,
C. S. Davies  and
R. E. Sinden
A. J. Hamilton
Affiliation:
Molecular and Cellular Parasitology Research Group, Department of Pure and Applied Biology, Imperial College, London SW7 2BB
C. S. Davies
Affiliation:
Molecular and Cellular Parasitology Research Group, Department of Pure and Applied Biology, Imperial College, London SW7 2BB
R. E. Sinden
Affiliation:
Molecular and Cellular Parasitology Research Group, Department of Pure and Applied Biology, Imperial College, London SW7 2BB
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Summary

A monoclonal antibody against the circumsporozoite proteins of the Plasmodium berghei sporozoite was used to trace the synthesis and expression of these proteins, via the Lowicryl immunogold technique, within the developing oocyst. The proteins were detected on the endoplasmic reticulum of the oocyst and were present in the sporozoite membranes at the point of their formation.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 2 , April 1988 , pp. 273 - 280
Copyright
Copyright © Cambridge University Press 1988

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