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Expressed sequence tag analysis of Sarcoptes scabiei

Published online by Cambridge University Press:  09 October 2003

E. L. LJUNGGREN ,
D. NILSSON  and
J. G. MATTSSON
E. L. LJUNGGREN
Affiliation:
Department of Parasitology (SWEPAR), National Veterinary Institute and Swedish University of Agricultural Sciences, SE-751 89 Uppsala, Sweden
D. NILSSON
Affiliation:
Center for Genomics and Bioinformatics, Karolinska Institute, Berzeliusv 35, SE-17177 Stockholm, Sweden
J. G. MATTSSON
Affiliation:
Department of Parasitology (SWEPAR), National Veterinary Institute and Swedish University of Agricultural Sciences, SE-751 89 Uppsala, Sweden
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Abstract

Sarcoptes scabiei is an important parasitic mite in both man and animals. Little is known about the molecular interactions between this pathogen and its host. This is in part explained by the paucity of mite-derived material, including antigens. To extend the knowledge of the molecular repertoire in S. scabiei, we have performed a gene survey by an expressed sequence tag (EST) analysis. A total of 1020 ESTs were generated from an S. scabiei cDNA library. The average sequence read was 510 bp after editing and the overall sequencing success was 89%. Clustering of the sequences resulted in 76 clusters, comprising 36% of the ESTs. Sequence similarity searches showed that almost half of the S. scabiei ESTs could be assigned a putative identity. Many of these transcripts shared similarity with genes involved in basic metabolism and cellular organization. In the data set we also identified several proteases and other types of potential allergens implicated in various disease mechanisms. A relatively large fraction of the ESTs coded for different proteins carrying protease inhibitor-like domains. The clones with no similarity to previously identified genes constituted 11% of our transcripts. The EST data generated in this study will be a valuable resource in further studies of the biology of S. scabiei and in the identification of genes that can serve as potential targets in the control of the parasite.

Keywords

Sarcoptes scabieiscabiesmangeESTgene expressionproteases
Type
Research Article
Information
Parasitology , Volume 127 , Issue 2 , August 2003 , pp. 139 - 145
Copyright
2003 Cambridge University Press

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