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Experimental mixed infection of Leishmania (Leishmania) amazonensis and Leishmania (L.) infantum in hamsters (Mesocricetus auratus)

Published online by Cambridge University Press:  10 May 2017

JORDANNA LUÍZA DE LIMA CELESTE ,
ANA PAULA VENUTO MOURA ,
JOÃO CARLOS FRANÇA-SILVA ,
GABRIELA MATOS DE SOUSA ,
SORAIA OLIVEIRA SILVA ,
MARIA NORMA MELO ,
WAGNER LUIZ TAFURI ,
CAROLINA CARVALHO SOUZA  and
HÉLIDA MONTEIRO DE ANDRADE
JORDANNA LUÍZA DE LIMA CELESTE
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31·270-901, Belo Horizonte, Minas Gerais, Brazil
ANA PAULA VENUTO MOURA
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31·270-901, Belo Horizonte, Minas Gerais, Brazil Departamento de Biologia Geral, Universidade Estadual de Montes Claros, CEP: 39·401-089, Montes Claros, Minas Gerais, Brazil
JOÃO CARLOS FRANÇA-SILVA
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31·270-901, Belo Horizonte, Minas Gerais, Brazil
GABRIELA MATOS DE SOUSA
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31·270-901, Belo Horizonte, Minas Gerais, Brazil
SORAIA OLIVEIRA SILVA
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31·270-901, Belo Horizonte, Minas Gerais, Brazil
MARIA NORMA MELO
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31·270-901, Belo Horizonte, Minas Gerais, Brazil
WAGNER LUIZ TAFURI
Affiliation:
Departamento de Patologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31·270-901, Belo Horizonte, Minas Gerais, Brazil
CAROLINA CARVALHO SOUZA
Affiliation:
Departamento de Patologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31·270-901, Belo Horizonte, Minas Gerais, Brazil Centro de Ciências Biológicas e da Saúde, CCBS, Universidade Federal do Oeste da Bahia, UFOB, - CEP: 47·808-020, Barreiras, Bahia, Brazil
HÉLIDA MONTEIRO DE ANDRADE*
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31·270-901, Belo Horizonte, Minas Gerais, Brazil
*
*Corresponding author: Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31·270-901, Belo Horizonte, Minas Gerais, Brazil. E-mail: helidandrade@gmail.com
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Summary

In South America, visceral leishmaniasis is frequently caused by Leishmania infantum and, at an unknown frequency, by Leishmania amazonensis. Therefore, mixed infections with these organisms are possible. Mixed infections might affect the clinical course, immune response, diagnosis, treatment and epidemiology of the disease. Here we describe the clinical course of mixed infections with L. amazonensis and L. infantum in a hamster model. We show that mixed infections are associated with more severe clinical disease than infection with L. amazonensis or L. infantum alone. In spleens with mixed infections, L. infantum outcompeted L. amazonensis in the tissue, but not in culture from tissue. We found increased levels of IgG in animals infected with L. infantum. Although more than 30 bands were revealed in a Western blot, the highest immunogenicity was observed with proteins having molecular masses of 95 and 90 kDa, whereas proteins with molecular masses of lower than 50 kDa were reactive frequently with serum from hamsters infected with L. amazonensis, and proteins with molecular masses of 80 and 70 kDa were reactive only with serum from hamsters infected with L. infantum. This finding has important implications regarding the biology of Leishmania and humoral immune responses to infections with these organisms.

Keywords

experimental infectionLeishmania infantumLeishmania amazonensismixed infectionclinical coursehumoral immune response
Type
Research Article
Information
Parasitology , Volume 144 , Issue 9 , August 2017 , pp. 1191 - 1202
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Copyright
Copyright © Cambridge University Press 2017 

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