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Enzyme variation in pathotypes of the potato cyst nematodes Globodera rostochiensis and G. pallida

Published online by Cambridge University Press:  06 April 2009

P. C. Fox  and
H. J. Atkinson
P. C. Fox
Affiliation:
Department of Pure and Applied Zoology, The University of Leeds, Leeds LS2 9JT
H. J. Atkinson
Affiliation:
Department of Pure and Applied Zoology, The University of Leeds, Leeds LS2 9JT
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Summary

A total of 65 enzymes in the 8 European pathotypes of the potato cyst nematodes Globodera pallida and G. rostochiensis were examined by isoelectric focusing (IEF) for possible polymorphic variants which may aid rapid pathotype recognition. Quantitative variation was seen with glucose oxidase (EC 1.1.3.4), with high levels of this enzyme present in G. rostochiensis Ro3. Mannose phosphate isomerase (EC 5.3.1.8) also varied between the pathotypes with G. rostochiensis Ro4 and Ro5 differing from the other pathotypes in the relative absorbance of some bands. Phosphoglucomutase was examined on a narrow-range pH gel and additional differences to those described previously were detected.

Type
Research Article
Information
Parasitology , Volume 91 , Issue 3 , December 1985 , pp. 499 - 506
Copyright
Copyright © Cambridge University Press 1985

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