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The effects of larval age and cold storage on the ecdysis of infective Haemonchus contortus cayugensis larvae

Published online by Cambridge University Press:  06 April 2009

J. Owen D. Slocombe  and
J. H. Whitlock
J. Owen D. Slocombe
Affiliation:
Department of Pathology, New York State Veterinary College, Cornell University, Ithaca, N.Y.
J. H. Whitlock
Affiliation:
Department of Pathology, New York State Veterinary College, Cornell University, Ithaca, N.Y.
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Extract

Infective Haemonchus contortus cayugensis larvae were harvested from faecal cultures, cleaned and stored at 4°C. By means of small sample runs, exsheathment rates were determined for batches of clean larvae prior to and during storage. The larvae were stimulated to exsheath at 40°C in 0·02 m-Na2B4O7. 10H2O into which a gas mixture of 40% CO2–60% N2 was bubbled. A 20 and 60 min exsheathing time produced some and no decrease in exsheathment rates respectively for freshly cleaned unstored larvae and larvae stored 1–12 weeks. In all instances there was a reduction in the exsheathment rate 24 h after the larvae were placed in cold storage. This was considered a result of a cold shock from which the larvae did not recover until after the fourth day.

Type
Research Article
Information
Parasitology , Volume 61 , Issue 2 , October 1970 , pp. 267 - 271
Copyright
Copyright © Cambridge University Press 1970

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References

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